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学科主题基础医学
Gene promoter methylation patterns throughout the process of cervical carcinogenesis
Yang, Nan1,2; Nijhuis, Esther R.1; Volders, Haukeline H.1; Eijsink, Jasper J. H.1; Lendvai, Agnes1; Zhang, Bo2; Hollema, Harry3; Schuuring, Ed3; Wisman, G. Bea A.1; van der Zee, Ate G. J.1
关键词Methylation cervical (intraepithelial) neoplasia DAPK CCNA1 CADM1
刊名CELLULAR ONCOLOGY
2010
DOI10.3233/CLO-2009-0510
32期:1-2页:131-143
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology ; Pathology
研究领域[WOS]Oncology ; Cell Biology ; Pathology
关键词[WOS]ABERRANT DNA METHYLATION ; HUMAN-PAPILLOMAVIRUS DNA ; SQUAMOUS-CELL CARCINOMA ; UTERINE CERVIX ; CANCER ; HYPERMETHYLATION ; NEOPLASIA ; PCR ; PREVALENCE ; EXPRESSION
英文摘要

Objectives: To determine methylation status of nine genes, previously described to be frequently methylated in cervical cancer, in squamous intraepithelial lesions (SIL).

Methods: QMSP was performed in normal cervix, low-grade ( L) SIL, high-grade (H) SIL, adenocarcinomas and squamous cell cervical cancers, and in corresponding cervical scrapings.

Results: Only CCNA1 was never methylated in normal cervices and rarely in LSILs. All other genes showed methylation in normal cervices, with CALCA, SPARC and RAR-beta(2) at high levels. Methylation frequency of 6 genes (DAPK, APC, TFPI2, SPARC, CCNA1 and CADM1) increased with severity of the underlying cervical lesion. DAPK showed the highest increase in methylation frequency between LSIL and HSIL (10% vs. 40%, p < 0.05), while CCNA1 and TFPI2 were most prominently methylated in cervical cancers compared to HSILs (25% vs. 52%, p < 0.05, 30% vs. 58%, p < 0.05). CADM1 methylation in cervical cancers was related to depth of invasion ( p < 0.05) and lymph vascular space involvement ( p < 0.01), suggesting a role in invasive potential of cervical cancers. Methylation ratios in scrapings reflected methylation status of the underlying lesions ( p < 0.05).

Conclusion: Methylation of previously reported cervical cancer specific genes frequently occurs in normal epithelium. However, frequency of methylation increases during cervical carcinogenesis, with CCNA1 and DAPK as the best markers to distinguish normal/LSIL from HSIL/cancer lesions.

语种英语
WOS记录号WOS:000276461800012
项目编号RUG 2004-3161
资助机构Dutch Cancer Society (NKB)
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50288
专题北京大学基础医学院_病理学系
北京大学第三临床医学院_总务处
作者单位1.Univ Groningen, Dept Gynecol Oncol, Univ Med Ctr Groningen, NL-9700 RB Groningen, Netherlands
2.Peking Univ, Dept Pathol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Univ Groningen, Dept Pathol, Univ Med Ctr Groningen, NL-9700 RB Groningen, Netherlands
推荐引用方式
GB/T 7714
Yang, Nan,Nijhuis, Esther R.,Volders, Haukeline H.,et al. Gene promoter methylation patterns throughout the process of cervical carcinogenesis[J]. CELLULAR ONCOLOGY,2010,32(1-2):131-143.
APA Yang, Nan.,Nijhuis, Esther R..,Volders, Haukeline H..,Eijsink, Jasper J. H..,Lendvai, Agnes.,...&van der Zee, Ate G. J..(2010).Gene promoter methylation patterns throughout the process of cervical carcinogenesis.CELLULAR ONCOLOGY,32(1-2),131-143.
MLA Yang, Nan,et al."Gene promoter methylation patterns throughout the process of cervical carcinogenesis".CELLULAR ONCOLOGY 32.1-2(2010):131-143.
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