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Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells
Ma, Dong-Xu; Shi, Nian-Qiu; Qi, Xian-Rong
关键词Cell-penetrating Peptides Endocytosis Heparan Sulfate Proteoglycans Macropinocytosis Nona-arginine
刊名INTERNATIONAL JOURNAL OF PHARMACEUTICS
2011-10-31
DOI10.1016/j.ijpharm.2011.08.001
419期:1-2页:200-208
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Foundation of the Ministry of Education of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Foundation of the Ministry of Education of China
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]TAT-FUSION PROTEINS ; HUMAN IMMUNODEFICIENCY VIRUS ; INTRACELLULAR DELIVERY ; PLASMA-MEMBRANE ; CYTOSOLIC DELIVERY ; LOW-TEMPERATURE ; DRUG-DELIVERY ; MACROPINOCYTOSIS ; INTERNALIZATION ; ENDOCYTOSIS
英文摘要

The application of cell-penetrating peptides (CPPs) for delivering various cargo molecules with biological functions into cells has gained much attention in recent years. However, the internalization mechanisms and delivery properties of CPP-cargo remains controversial. In this study, low- and high-molecular-weight cargoes attached to arginine-rich CPPs were employed: the former was the fluorescein isothiocyanate-labeled nona-arginine (CPP-FITC), and the latter was the fluorescently labeled nona-arginine-avidin complex (CPP-avidin). We measured the intracellular trafficking of CPP-FITC and CPP-avidin in four cancer cell lines in a series of microenvironments altered by the presence or absence of serum, different temperatures and different incubation times. The results revealed that CPP-cargo delivery exhibited no specificity toward any cell line, but the levels were found to be related to cell type and cargo. Furthermore, their endocytic mechanisms were investigated via incubation with related endocytic inhibitors. Two different types of CPP-cargo were required to cross the plasma membrane to bind to cell surface-associated heparan sulfate proteoglycans in a time-dependent manner. CPPs and small cargoes attached to CPP may enter cells rapidly via direct translocation in addition to the endocytic route. Translocation of large components linked to CPP tended to be mediated by macropinocytosis in an energy-dependent manner with slower rates for larger compounds. In contrast, the clathrin-dependent pathway is not essential to the translocation of either type of CPP-cargo. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号30772665 ; 30970785 ; 2007CB935801 ; 20100001110056
资助者National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Foundation of the Ministry of Education of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Foundation of the Ministry of Education of China
WOS记录号WOS:000296307000025
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50294
专题北京大学药学院_药剂学系
作者单位Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100191, Peoples R China
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GB/T 7714
Ma, Dong-Xu,Shi, Nian-Qiu,Qi, Xian-Rong. Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2011,419(1-2):200-208.
APA Ma, Dong-Xu,Shi, Nian-Qiu,&Qi, Xian-Rong.(2011).Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells.INTERNATIONAL JOURNAL OF PHARMACEUTICS,419(1-2),200-208.
MLA Ma, Dong-Xu,et al."Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells".INTERNATIONAL JOURNAL OF PHARMACEUTICS 419.1-2(2011):200-208.
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