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学科主题: 药学
题名:
Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells
作者: Ma, Dong-Xu; Shi, Nian-Qiu; Qi, Xian-Rong
关键词: Cell-penetrating peptides ; Endocytosis ; Heparan sulfate proteoglycans ; Macropinocytosis ; Nona-arginine
刊名: INTERNATIONAL JOURNAL OF PHARMACEUTICS
发表日期: 2011-10-31
DOI: 10.1016/j.ijpharm.2011.08.001
卷: 419, 期:1-2, 页:200-208
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: TAT-FUSION PROTEINS ; HUMAN IMMUNODEFICIENCY VIRUS ; INTRACELLULAR DELIVERY ; PLASMA-MEMBRANE ; CYTOSOLIC DELIVERY ; LOW-TEMPERATURE ; DRUG-DELIVERY ; MACROPINOCYTOSIS ; INTERNALIZATION ; ENDOCYTOSIS
英文摘要:

The application of cell-penetrating peptides (CPPs) for delivering various cargo molecules with biological functions into cells has gained much attention in recent years. However, the internalization mechanisms and delivery properties of CPP-cargo remains controversial. In this study, low- and high-molecular-weight cargoes attached to arginine-rich CPPs were employed: the former was the fluorescein isothiocyanate-labeled nona-arginine (CPP-FITC), and the latter was the fluorescently labeled nona-arginine-avidin complex (CPP-avidin). We measured the intracellular trafficking of CPP-FITC and CPP-avidin in four cancer cell lines in a series of microenvironments altered by the presence or absence of serum, different temperatures and different incubation times. The results revealed that CPP-cargo delivery exhibited no specificity toward any cell line, but the levels were found to be related to cell type and cargo. Furthermore, their endocytic mechanisms were investigated via incubation with related endocytic inhibitors. Two different types of CPP-cargo were required to cross the plasma membrane to bind to cell surface-associated heparan sulfate proteoglycans in a time-dependent manner. CPPs and small cargoes attached to CPP may enter cells rapidly via direct translocation in addition to the endocytic route. Translocation of large components linked to CPP tended to be mediated by macropinocytosis in an energy-dependent manner with slower rates for larger compounds. In contrast, the clathrin-dependent pathway is not essential to the translocation of either type of CPP-cargo. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30772665 ; 30970785 ; 2007CB935801 ; 20100001110056
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Foundation of the Ministry of Education of China
WOS记录号: WOS:000296307000025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50294
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100191, Peoples R China

Recommended Citation:
Ma, Dong-Xu,Shi, Nian-Qiu,Qi, Xian-Rong. Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2011,419(1-2):200-208.
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