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学科主题: 基础医学
题名:
Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium
作者: Zhou, Hong1,2; Lu, Ning1,2; Chen, Zhi-Qiang3; Song, Qian-Liu1,2; Yu, He-Ming4; Li, Xue-Jun1,2
刊名: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
发表日期: 2009-02-01
DOI: 10.1111/j.1742-7843.2008.00348.x
卷: 104, 期:2, 页:164-170
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; CANCER CELLS ; POPULATION-DENSITY ; CARCINOMA-CELLS ; APOPTOSIS ; EXPRESSION ; GROWTH ; ALPHA ; STIMULATION ; REQUIREMENT
英文摘要:

Cells growing in high density were observed to undergo a variety of responses due to cell-cell contact, pericellular hypoxia, etc. In order to investigate the influence of cell density on cell proliferation and adhesion and to elucidate possible mechanisms, we tested the growth ability of human prostate tumour (PC-3M) cells in dense culture and the influences of density on cell adhesion. Our results demonstrate that increasing cell density exerted stress on PC-3M cells, which decreased cell proliferation in dense cultures, but tended to facilitate tumour metastasis since cell adhesion ability was elevated and the cells showed an increased growth rate after being moved to a favourable growth environment. We conclude that higher cell density-mediated pericellular hypoxia was an important factor inducing expression of the intrinsic hypoxia marker osteopontin, another mechanism contributing to cell adhesion enhancement in PC-3M cells. In addition, cell density enhanced adhesion ability due to the activation of p38 mitogen-activated protein kinase (p38 MAPK) and protein kinase C. Intracellular calcium also played positive roles at least partially through activating p38 MAPK.

语种: 英语
所属项目编号: 30171090 ; 39770286 ; 2001AA235061 ; 2002AA2Z343C ; 20020001082
项目资助者: National Natural Science Foundation of China ; 863 Program of Ministry of Science and Technology of China ; Research Fund from Ministry of Education of China
WOS记录号: WOS:000262487400012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50303
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing, Peoples R China

Recommended Citation:
Zhou, Hong,Lu, Ning,Chen, Zhi-Qiang,et al. Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium[J]. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY,2009,104(2):164-170.
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