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学科主题基础医学
Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium
Zhou, Hong1,2; Lu, Ning1,2; Chen, Zhi-Qiang3; Song, Qian-Liu1,2; Yu, He-Ming4; Li, Xue-Jun1,2
刊名BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
2009-02-01
DOI10.1111/j.1742-7843.2008.00348.x
104期:2页:164-170
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; CANCER CELLS ; POPULATION-DENSITY ; CARCINOMA-CELLS ; APOPTOSIS ; EXPRESSION ; GROWTH ; ALPHA ; STIMULATION ; REQUIREMENT
英文摘要

Cells growing in high density were observed to undergo a variety of responses due to cell-cell contact, pericellular hypoxia, etc. In order to investigate the influence of cell density on cell proliferation and adhesion and to elucidate possible mechanisms, we tested the growth ability of human prostate tumour (PC-3M) cells in dense culture and the influences of density on cell adhesion. Our results demonstrate that increasing cell density exerted stress on PC-3M cells, which decreased cell proliferation in dense cultures, but tended to facilitate tumour metastasis since cell adhesion ability was elevated and the cells showed an increased growth rate after being moved to a favourable growth environment. We conclude that higher cell density-mediated pericellular hypoxia was an important factor inducing expression of the intrinsic hypoxia marker osteopontin, another mechanism contributing to cell adhesion enhancement in PC-3M cells. In addition, cell density enhanced adhesion ability due to the activation of p38 mitogen-activated protein kinase (p38 MAPK) and protein kinase C. Intracellular calcium also played positive roles at least partially through activating p38 MAPK.

语种英语
WOS记录号WOS:000262487400012
项目编号30171090 ; 39770286 ; 2001AA235061 ; 2002AA2Z343C ; 20020001082
资助机构National Natural Science Foundation of China ; 863 Program of Ministry of Science and Technology of China ; Research Fund from Ministry of Education of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50303
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing, Peoples R China
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GB/T 7714
Zhou, Hong,Lu, Ning,Chen, Zhi-Qiang,et al. Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium[J]. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY,2009,104(2):164-170.
APA Zhou, Hong,Lu, Ning,Chen, Zhi-Qiang,Song, Qian-Liu,Yu, He-Ming,&Li, Xue-Jun.(2009).Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium.BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY,104(2),164-170.
MLA Zhou, Hong,et al."Osteopontin Mediates Dense Culture-Induced Proliferation and Adhesion of Prostate Tumour Cells: Role of Protein Kinase C, p38 Mitogen-Activated Protein Kinase and Calcium".BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY 104.2(2009):164-170.
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