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Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres
Feng, L1; Qi, XR1; Zhou, XJ1; Maitani, Y1; Wang, SC1; Jiang, Y1; Nagai, T1
关键词Hepatitis b Surface Antigen (Hbsag) Single-dose Vaccine Plga Microspheres Controlled Release Immunogenicity
刊名JOURNAL OF CONTROLLED RELEASE
2006-05-01
DOI10.1016/j.jconrel.2006.01.012
112期:1页:35-42
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]BIODEGRADABLE POLYMER MICROSPHERES ; IN-VITRO RELEASE ; ACID) MICROSPHERES ; ADJUVANT ; PROTEIN ; POLY(LACTIDE-CO-GLYCOLIDE) ; IMMUNOGENICITY ; MICROPARTICLES ; STABILIZATION ; DEGRADATION
英文摘要

The objective of the study was to investigate the feasibility of a single-dose hepatitis B vaccine based on three kinds of poly (D, L)-lactide-coglicolide acid (PLGA) microspheres. PLGA microspheres loaded with recombinant hepatitis B surface antigen (HBsAg) were formulated using a double emulsion microencapsulation technique. The pharmaceutical characteristics of size, surface morphology, protein loading efficiency, antigen integrity, release of HBsAg-loaded PLGA microspheres and degradation of the polymer in vitro were evaluated. The degradation of the polymer corresponded with the composition of the polymer (lactide /glycolide ratio), molecular weight of the polymer (viscosity) and morphology of the microspheres. These PLGA microspheres were able to continuously release antigen under conditions that mimic the environment in vivo. The single subcutaneous injection of RBsAg-loaded PLGA50/50 microspheres, PLGA75/25 microspheres and a mixture of PLGA50/50, PLGA75/ 25, and PLGA50/50-COOH microspheres in mice resulted in comparable serum antibody titers to those of three injections of the conventional aluminum adjuvant formulated HBsAg vaccine. Based on these findings in vitro and in vivo, it was concluded that HBsAg was successfully loaded into the PLGA microspheres, which can auto-boost an immune response, and the HBsAg-loaded PLGA microsphere is a promising candidate for the controlled delivery of a vaccine. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000237263100004
Citation statistics
Cited Times:83[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50308
Collection北京大学药学院_药剂学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.NCPC New Drug R&D Co Ltd, Shijiazhuang 050015, Hebei, Peoples R China
3.Hoshi Univ, Inst Med Chem, Tokyo 1428501, Japan
4.NCPC, Gene Tech Biotechnol Dev Co Ltd, Shijianhuang 050035, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Feng, L,Qi, XR,Zhou, XJ,et al. Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres[J]. JOURNAL OF CONTROLLED RELEASE,2006,112(1):35-42.
APA Feng, L.,Qi, XR.,Zhou, XJ.,Maitani, Y.,Wang, SC.,...&Nagai, T.(2006).Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres.JOURNAL OF CONTROLLED RELEASE,112(1),35-42.
MLA Feng, L,et al."Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres".JOURNAL OF CONTROLLED RELEASE 112.1(2006):35-42.
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