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学科主题: 药学
题名:
Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres
作者: Feng, L1; Qi, XR1; Zhou, XJ1; Maitani, Y1; Wang, SC1; Jiang, Y1; Nagai, T1
关键词: hepatitis B surface antigen (HBsAg) ; single-dose vaccine ; PLGA microspheres ; controlled release ; immunogenicity
刊名: JOURNAL OF CONTROLLED RELEASE
发表日期: 2006-05-01
DOI: 10.1016/j.jconrel.2006.01.012
卷: 112, 期:1, 页:35-42
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: BIODEGRADABLE POLYMER MICROSPHERES ; IN-VITRO RELEASE ; ACID) MICROSPHERES ; ADJUVANT ; PROTEIN ; POLY(LACTIDE-CO-GLYCOLIDE) ; IMMUNOGENICITY ; MICROPARTICLES ; STABILIZATION ; DEGRADATION
英文摘要:

The objective of the study was to investigate the feasibility of a single-dose hepatitis B vaccine based on three kinds of poly (D, L)-lactide-coglicolide acid (PLGA) microspheres. PLGA microspheres loaded with recombinant hepatitis B surface antigen (HBsAg) were formulated using a double emulsion microencapsulation technique. The pharmaceutical characteristics of size, surface morphology, protein loading efficiency, antigen integrity, release of HBsAg-loaded PLGA microspheres and degradation of the polymer in vitro were evaluated. The degradation of the polymer corresponded with the composition of the polymer (lactide /glycolide ratio), molecular weight of the polymer (viscosity) and morphology of the microspheres. These PLGA microspheres were able to continuously release antigen under conditions that mimic the environment in vivo. The single subcutaneous injection of RBsAg-loaded PLGA50/50 microspheres, PLGA75/25 microspheres and a mixture of PLGA50/50, PLGA75/ 25, and PLGA50/50-COOH microspheres in mice resulted in comparable serum antibody titers to those of three injections of the conventional aluminum adjuvant formulated HBsAg vaccine. Based on these findings in vitro and in vivo, it was concluded that HBsAg was successfully loaded into the PLGA microspheres, which can auto-boost an immune response, and the HBsAg-loaded PLGA microsphere is a promising candidate for the controlled delivery of a vaccine. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000237263100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50308
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.NCPC New Drug R&D Co Ltd, Shijiazhuang 050015, Hebei, Peoples R China
3.Hoshi Univ, Inst Med Chem, Tokyo 1428501, Japan
4.NCPC, Gene Tech Biotechnol Dev Co Ltd, Shijianhuang 050035, Hebei, Peoples R China

Recommended Citation:
Feng, L,Qi, XR,Zhou, XJ,et al. Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres[J]. JOURNAL OF CONTROLLED RELEASE,2006,112(1):35-42.
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