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学科主题: 口腔医学
题名:
Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats
作者: Kou, Xiao-Xing1,2,3; Li, Chen-Shuang1; He, Dan-Qing1; Wang, Xue-Dong1; Hao, Ting2,3; Meng, Zhen2,3; Zhou, Yan-Heng1; Gan, Ye-Hua2,3
刊名: JOURNAL OF IMMUNOLOGY
发表日期: 2015-03-15
DOI: 10.4049/jimmunol.1303188
卷: 194, 期:6, 页:2810-2818
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: ENDOMETRIAL STROMAL CELLS ; RHEUMATOID-ARTHRITIS ; ALTERNATIVE ACTIVATION ; OVARIECTOMIZED RATS ; DISEASE-ACTIVITY ; KAPPA-B ; EXPRESSION ; PAIN ; TISSUE ; PATHOGENESIS
英文摘要:

Macrophages play a major role in joint inflammation. Estrogen is involved in rheumatoid arthritis and temporomandibular disorders. However, the underlying mechanism is still unclear. This study was done to verify and test how estrogen affects M1/M2-like macrophage polarization and then contributes to joint inflammation. Female rats were ovariectomized and treated with increasing doses of 17 beta-estradiol for 10 d and then intra-articularly injected with CFA to induce temporomandibular joint (TMJ) inflammation. The polarization of macrophages and expression of cadherin-11 was evaluated at 24 h after the induction of TMJ inflammation and after blocking cadherin-11 or estrogen receptors. NR8383 macrophages were treated with estradiol and TNF-alpha, with or without blocking cadherin-11 or estrogen receptors, to evaluate the expression of the M1/M2-like macrophage-associated genes. We found that estradiol increased the infiltration of macrophages with a proinflammatory M1-like predominant profile in the synovium of inflamed TMJ. In addition, estradiol dose-dependently upregulated the expressions of the M1-associated proinflammatory factor inducible NO synthase (iNOS) but repressed the expressions of the M2-associated genes IL-10 and arginase in NR8383 macrophages. Furthermore, estradiol mainly promoted cadherin-11 expression in M1-like macrophages of inflamed TMJ. By contrast, blockage of cadherin-11 concurrently reversed estradiol-potentiated M1-like macrophage activation and TMJ inflammation, as well as reversed TNF-alpha-induced induction of inducible NO synthase and NO in NR8383 macrophages. The blocking of estrogen receptors reversed estradiol-potentiated M1-like macrophage activation and cadherin-11 expression. These results suggested that estradiol could promote M1-like macrophage activation through cadherin-11 to aggravate the acute inflammation of TMJs.

语种: 英语
所属项目编号: 81271173 ; 81300897
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000350755200040
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50309
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China

Recommended Citation:
Kou, Xiao-Xing,Li, Chen-Shuang,He, Dan-Qing,et al. Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats[J]. JOURNAL OF IMMUNOLOGY,2015,194(6):2810-2818.
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