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学科主题口腔医学
Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats
Kou, Xiao-Xing1,2,3; Li, Chen-Shuang1; He, Dan-Qing1; Wang, Xue-Dong1; Hao, Ting2,3; Meng, Zhen2,3; Zhou, Yan-Heng1; Gan, Ye-Hua2,3
刊名JOURNAL OF IMMUNOLOGY
2015-03-15
DOI10.4049/jimmunol.1303188
194期:6页:2810-2818
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Immunology
关键词[WOS]ENDOMETRIAL STROMAL CELLS ; RHEUMATOID-ARTHRITIS ; ALTERNATIVE ACTIVATION ; OVARIECTOMIZED RATS ; DISEASE-ACTIVITY ; KAPPA-B ; EXPRESSION ; PAIN ; TISSUE ; PATHOGENESIS
英文摘要

Macrophages play a major role in joint inflammation. Estrogen is involved in rheumatoid arthritis and temporomandibular disorders. However, the underlying mechanism is still unclear. This study was done to verify and test how estrogen affects M1/M2-like macrophage polarization and then contributes to joint inflammation. Female rats were ovariectomized and treated with increasing doses of 17 beta-estradiol for 10 d and then intra-articularly injected with CFA to induce temporomandibular joint (TMJ) inflammation. The polarization of macrophages and expression of cadherin-11 was evaluated at 24 h after the induction of TMJ inflammation and after blocking cadherin-11 or estrogen receptors. NR8383 macrophages were treated with estradiol and TNF-alpha, with or without blocking cadherin-11 or estrogen receptors, to evaluate the expression of the M1/M2-like macrophage-associated genes. We found that estradiol increased the infiltration of macrophages with a proinflammatory M1-like predominant profile in the synovium of inflamed TMJ. In addition, estradiol dose-dependently upregulated the expressions of the M1-associated proinflammatory factor inducible NO synthase (iNOS) but repressed the expressions of the M2-associated genes IL-10 and arginase in NR8383 macrophages. Furthermore, estradiol mainly promoted cadherin-11 expression in M1-like macrophages of inflamed TMJ. By contrast, blockage of cadherin-11 concurrently reversed estradiol-potentiated M1-like macrophage activation and TMJ inflammation, as well as reversed TNF-alpha-induced induction of inducible NO synthase and NO in NR8383 macrophages. The blocking of estrogen receptors reversed estradiol-potentiated M1-like macrophage activation and cadherin-11 expression. These results suggested that estradiol could promote M1-like macrophage activation through cadherin-11 to aggravate the acute inflammation of TMJs.

语种英语
所属项目编号81271173 ; 81300897
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000350755200040
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50309
Collection北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Kou, Xiao-Xing,Li, Chen-Shuang,He, Dan-Qing,et al. Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats[J]. JOURNAL OF IMMUNOLOGY,2015,194(6):2810-2818.
APA Kou, Xiao-Xing.,Li, Chen-Shuang.,He, Dan-Qing.,Wang, Xue-Dong.,Hao, Ting.,...&Gan, Ye-Hua.(2015).Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats.JOURNAL OF IMMUNOLOGY,194(6),2810-2818.
MLA Kou, Xiao-Xing,et al."Estradiol Promotes M1-like Macrophage Activation through Cadherin-11 To Aggravate Temporomandibular Joint Inflammation in Rats".JOURNAL OF IMMUNOLOGY 194.6(2015):2810-2818.
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