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学科主题: 临床医学
题名:
ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha
作者: Meng, QH1; Zhao, ZG1; Yan, M1; Zhou, LX1; Li, JY1; Kitt, C1; Bin, G1; Fan, SJ1
关键词: ERR-10 ; estrogen receptor ; transcription ; LXXLL ; co-repressor ; breast cancer
刊名: FEBS LETTERS
发表日期: 2004-10-08
DOI: 10.1016/j.febslet.2004.07.094
卷: 576, 期:1-2, 页:190-200
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: STEROID-HORMONE RECEPTORS ; BRCA1 INHIBITION ; MOLECULAR-CLONING ; BINDING PROTEIN ; COACTIVATOR ; MOTIF ; BETA ; SUPERFAMILY ; MECHANISMS ; DISTINCT
英文摘要:

Estrogen receptor-alpha (ER-alpha) is a nuclear transcriptional factor that is part of the nuclear receptor superfamily. In this study, we isolated and identified a new LXXLL-containing protein that interacts with the ER-alpha via a yeast two-hybrid assay. We have termed this protein estrogen receptor repressor-10 (ERR-10). The ERR-10 cDNA is predicted to encode a polypeptide of 94 amino acids, with a molecular mass of about 10 kDa. Although the ERR-10 mRNA transcript is expressed in a wide range of normal human tissues, higher expression levels are found in endocrinal tissues relative to other tissues. We have demonstrated, through immunoprecipitation, Western blot and GST pull-down assays, that ERR-10 associates with ER-alpha. Moreover, ERR-10 decreased 17beta-estrodial-induced activation of ER-alpha transcriptional activity in transient transfection assays of mammalian cells. The ERR-10 N-terminus, which resembles two LXXLL motifs, is essential for ER-alpha binding and repression activity. Estrogen modulation of estrogen-responsive gene expression was markedly blocked by ERR-10. These results suggest that ERR-10 is a novel mediator in ER transcriptional activation. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

语种: 英语
WOS记录号: WOS:000224607800038
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50318
Appears in Collections:北京大学临床肿瘤学院_病理科_期刊论文

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作者单位: 1.NIAAA, Div Liver Biol, NIH, Rockville, MD 20852 USA
2.Georgetown Univ, Med Ctr, Lombardi Comprehens Canc Ctr, Dept Oncol, Washington, DC 20057 USA
3.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Pathol, Beijing 100036, Peoples R China
4.Soochow Univ, Sch Radiol & Publ Hlth, Suzhou 215007, Peoples R China

Recommended Citation:
Meng, QH,Zhao, ZG,Yan, M,et al. ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha[J]. FEBS LETTERS,2004,576(1-2):190-200.
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