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ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha
Meng, QH1; Zhao, ZG1; Yan, M1; Zhou, LX1; Li, JY1; Kitt, C1; Bin, G1; Fan, SJ1
关键词Err-10 Estrogen Receptor Transcription Lxxll Co-repressor Breast Cancer
刊名FEBS LETTERS
2004-10-08
DOI10.1016/j.febslet.2004.07.094
576期:1-2页:190-200
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]STEROID-HORMONE RECEPTORS ; BRCA1 INHIBITION ; MOLECULAR-CLONING ; BINDING PROTEIN ; COACTIVATOR ; MOTIF ; BETA ; SUPERFAMILY ; MECHANISMS ; DISTINCT
英文摘要

Estrogen receptor-alpha (ER-alpha) is a nuclear transcriptional factor that is part of the nuclear receptor superfamily. In this study, we isolated and identified a new LXXLL-containing protein that interacts with the ER-alpha via a yeast two-hybrid assay. We have termed this protein estrogen receptor repressor-10 (ERR-10). The ERR-10 cDNA is predicted to encode a polypeptide of 94 amino acids, with a molecular mass of about 10 kDa. Although the ERR-10 mRNA transcript is expressed in a wide range of normal human tissues, higher expression levels are found in endocrinal tissues relative to other tissues. We have demonstrated, through immunoprecipitation, Western blot and GST pull-down assays, that ERR-10 associates with ER-alpha. Moreover, ERR-10 decreased 17beta-estrodial-induced activation of ER-alpha transcriptional activity in transient transfection assays of mammalian cells. The ERR-10 N-terminus, which resembles two LXXLL motifs, is essential for ER-alpha binding and repression activity. Estrogen modulation of estrogen-responsive gene expression was markedly blocked by ERR-10. These results suggest that ERR-10 is a novel mediator in ER transcriptional activation. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

语种英语
WOS记录号WOS:000224607800038
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50318
专题北京大学临床肿瘤学院_病理科
作者单位1.NIAAA, Div Liver Biol, NIH, Rockville, MD 20852 USA
2.Georgetown Univ, Med Ctr, Lombardi Comprehens Canc Ctr, Dept Oncol, Washington, DC 20057 USA
3.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Pathol, Beijing 100036, Peoples R China
4.Soochow Univ, Sch Radiol & Publ Hlth, Suzhou 215007, Peoples R China
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GB/T 7714
Meng, QH,Zhao, ZG,Yan, M,et al. ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha[J]. FEBS LETTERS,2004,576(1-2):190-200.
APA Meng, QH.,Zhao, ZG.,Yan, M.,Zhou, LX.,Li, JY.,...&Fan, SJ.(2004).ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha.FEBS LETTERS,576(1-2),190-200.
MLA Meng, QH,et al."ERR-10: a new repressor in transcriptional signaling activation of estrogen receptor-alpha".FEBS LETTERS 576.1-2(2004):190-200.
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