IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Hypochlorite-induced oxidative stress elevates the capability of HDL in promoting breast cancer metastasis
Niu, Chenguang1,2,3,4; Pan, Bing1,2,3,4; Ren, Hui1,2,3,4; Lv, Xiaofeng5,6; Zhao, Yangyu7,8; Yu, Baoqi1,2,3,4; He, Yubin5,6; Ma, Yijing1,2,3,4; Kong, Jinge1,2,3,4; Yu, Fangzhu1,2,3,4; Sun, Wen-bing9; Zhang, Youyi1,2,3,4; Willard, Belinda10; Zheng, Lemin1,2,3,4
关键词Breast Cancer Oxidative Stress Metastasis High-density Lipoprotein
刊名JOURNAL OF TRANSLATIONAL MEDICINE
2012-03-30
DOI10.1186/1479-5876-10-65
10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]HIGH-DENSITY-LIPOPROTEIN ; PROTEIN-KINASE-C ; ENDOTHELIAL-CELLS ; ADHESION MOLECULES ; SERUM-LIPIDS ; PKC-ALPHA ; CHOLESTEROL ; TARGET ; RISK ; ASSOCIATION
英文摘要

Background: Previous studies suggest that oxidative stress plays an important role in the development of breast cancer. There is a significant inverse relationship between HDL and the risk and mortality of breast cancer. However, it is well known that under conditions of oxidative stress, such as breast cancer, HDL can be oxidatively modifiedand these modifications may have an effect on the functions of HDL. The purpose of this study is to determine the different effects of normal and oxidized (caused by hypochlorite-induced oxidative stress) HDL on breast cancer cell metastasis.

Methods: Human breast cancer cell lines were treated with normal and hypochlorite-oxidized HDL, and then cell metastasis potency in vivo and the abilities of migration, invasion, adhesion to HUVEC and ECM in vitro were examined. Integrin expression and PKC activity were evaluated, and PKC inhibitor and PKC siRNA was applied.

Results: We found hypochlorite-oxidized HDL dramatically promotes breast cancer cell pulmonary metastasis (133.4% increase at P < 0.0 I for MDA-MB-231 by mammary fat pad injection; 164.3% increase at P < 0.01 for MCF7 by tail vein injection) and hepatic metastasis (420% increase at P < 0.0 l for MDA-MB-231 by mammary fat padinjection; 1840% fold increase at P < 0.001 for MCF7 by tail vein injection) in nude mice, and stimulates higher cell invasion (85.1% increase at P < 0.00 I for MDA-MB-231; 88.8% increase at P < 0.00 I for MCF7;), TC-HUVEC adhesion (43.4% increase at P < 0.00 I for MDA-MB-231; 35.2% increase at P < 0.00 l for MCF7), and TC-ECM attachment (41.0% increase at P < 0.00 I for MDA-MB-231; 26.7% increase at P < 0.05 for MCF7) in vitro compared with normal HDL. The data also shows that the PKC pathway is involved in the abnormal actions of hypochlorite-oxidized HDL.

Conclusions: Our study demonstrated that HDL under hypochlorite- induced oxidative stress stimulates breast cancer cell migration, invasion, adhesion to HUVEC and ECM, thereby promoting metastasis of breast cancer. These results suggest that HDL-based treatments should be considered for treatment of breast cancer patients.

语种英语
WOS记录号WOS:000303871900001
项目编号2010CB912504 ; 2011CB503900 ; 30900595 ; 30821001 ; 81172500 ; 81170101 ; 7102104 ; 7122106 ; 2009J01112 ; 20090001120039 ; 20100001120034
资助机构"973" National ST Major Project ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Natural Science Foundation of Fujian ; State Education Ministry of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50321
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Syst Biomed, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
5.Mil Gen Hosp Beijing, Dept Cardiovasc Med, Beijing 100700, Peoples R China
6.Mil Gen Hosp Beijing, Dept Endocrinol, Beijing 100700, Peoples R China
7.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
8.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100191, Peoples R China
9.Capital Med Univ, Beijing Chao Yang Hosp, Dept Hepatobiliary Surg, Beijing 100043, Peoples R China
10.Cleveland Clin, Prote Lab, Cleveland, OH 44195 USA
推荐引用方式
GB/T 7714
Niu, Chenguang,Pan, Bing,Ren, Hui,et al. Hypochlorite-induced oxidative stress elevates the capability of HDL in promoting breast cancer metastasis[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2012,10.
APA Niu, Chenguang.,Pan, Bing.,Ren, Hui.,Lv, Xiaofeng.,Zhao, Yangyu.,...&Zheng, Lemin.(2012).Hypochlorite-induced oxidative stress elevates the capability of HDL in promoting breast cancer metastasis.JOURNAL OF TRANSLATIONAL MEDICINE,10.
MLA Niu, Chenguang,et al."Hypochlorite-induced oxidative stress elevates the capability of HDL in promoting breast cancer metastasis".JOURNAL OF TRANSLATIONAL MEDICINE 10(2012).
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