IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Inhibition of central Na+/H+ exchanger type 3 can alleviate sleep apnea in Sprague-Dawley rats
Wang Qimin; Zhou Rong; Zhang Cheng; Dong Hui; Ma Jing; Wang Guangfa
关键词na+/h+ Exchanger 3 Rat Sleep Apnea Sleep Monitoring
刊名CHINESE MEDICAL JOURNAL
2014-01-05
DOI10.3760/cma.j.issn.0366-6999.20131693
127期:1页:48-53
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]BRAIN-STEM ; CENTRAL CHEMOSENSITIVITY ; MEDULLARY NEURONS ; REM-SLEEP ; NHE3 ; CO2 ; MECHANISMS ; REGIONS
英文摘要

Background Recent studies showed the central Na+/H+ exchanger type 3 (NHE3) has a close relationship with ventilation control. The objective of the study is to investigate the role of NHE3 in sleep apnea in Sprague-Dawley (SD) rats.

Methods A sleep study was performed on 20 male SD rats to analyze the correlation between the sleep apneic events and total NHE3 protein content and inactive NHE3(pS552) in the brainstem measured by Western blotting. Another 20 adult male SD rats received 3 days of sleep and respiration monitoring for 6 hours a day, with adaption on the first day, 0.5% DMSO microinjection into the fourth ventricle on the second day, and AVE0657 (specific inhibitor of NHE3) microinjection on the third day. Rats were divided into two groups with injection of 5 mu mol/L or 8 mu mol/L AVE0657 before the sleep study. The effects of AVE0657 on sleep apnea and sleep structure of rats were analyzed through self-control.

Results The total post-sigh apnea index (TPSAI) and post-sigh apnea index in non-rapid eye movement (NREM) sleep (NPSAI) and total apnea index (AI) in NREM sleep (NAI) were negatively correlated with NHE3(pS552) protein contents in the brainstem (r=-0.534, -0.547 and -0.505, respectively, P<0.05). The spontaneous apnea index in REM sleep (RSPAI) was positively correlated with the level of NHE3(pS552) protein expression in the brainstem (r=0.556, P<0.05). However, the sleep AI had no relationship with total NHE3 protein. Compared with the blank control and microinjection of 0.5% DMSO, 5 mu mol/L AVE0657 significantly reduced the total AI and NPSAI (both P<0.05) without a significant effect on sleep architecture. In contrast to blank control and microinjection of 0.5% DMSO, injection of 8 mu mol/L AVE0657 significantly reduced the AI and PSAI in NREM and REM sleep (all P<0.05).

Conclusions The severity of sleep apnea was negatively correlated with central inactive NHE3. A specific inhibitor of NHE3 decreased the sleep AI. Thus, our results indicate that central NHE3 might be a molecular target for sleep apnea treatment, whose inhibitors may be potential therapeutic drugs for sleep apnea.

语种英语
WOS记录号WOS:000330606800009
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50322
专题北京大学第一临床医学院
北京大学第三临床医学院_胸外科
北京大学第一临床医学院_呼吸科
作者单位Peking Univ, Dept Resp & Crit Care Med, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Wang Qimin,Zhou Rong,Zhang Cheng,et al. Inhibition of central Na+/H+ exchanger type 3 can alleviate sleep apnea in Sprague-Dawley rats[J]. CHINESE MEDICAL JOURNAL,2014,127(1):48-53.
APA Wang Qimin,Zhou Rong,Zhang Cheng,Dong Hui,Ma Jing,&Wang Guangfa.(2014).Inhibition of central Na+/H+ exchanger type 3 can alleviate sleep apnea in Sprague-Dawley rats.CHINESE MEDICAL JOURNAL,127(1),48-53.
MLA Wang Qimin,et al."Inhibition of central Na+/H+ exchanger type 3 can alleviate sleep apnea in Sprague-Dawley rats".CHINESE MEDICAL JOURNAL 127.1(2014):48-53.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang Qimin]的文章
[Zhou Rong]的文章
[Zhang Cheng]的文章
百度学术
百度学术中相似的文章
[Wang Qimin]的文章
[Zhou Rong]的文章
[Zhang Cheng]的文章
必应学术
必应学术中相似的文章
[Wang Qimin]的文章
[Zhou Rong]的文章
[Zhang Cheng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。