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学科主题: 口腔医学
题名:
Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice
作者: Su, Y-C1; Xiang, R-L1; Zhang, Y.1; Ding, C.2; Cong, X.1; Guo, X-H1; Yang, N-Y3; Hua, H.4; Wu, L-L1; Yu, G-Y3
关键词: adiponectin ; autoimmune sialoadenitis ; non-obese diabetic mice ; Sjogren&prime ; s syndrome
刊名: ORAL DISEASES
发表日期: 2014-11-01
DOI: 10.1111/odi.12197
卷: 20, 期:8, 页:744-755
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: ACTIVATED PROTEIN-KINASE ; NF-KAPPA-B ; SJOGRENS-SYNDROME ; PLASMA ADIPONECTIN ; SALIVARY-GLAND ; INSULIN-RESISTANCE ; RHEUMATOID-ARTHRITIS ; EPITHELIAL-CELLS ; ADULT-RAT ; TNF-ALPHA
英文摘要:

ObjectiveTo investigate a possible role of adiponectin in the pathogenesis of autoimmune sialoadenitis in non-obese diabetic (NOD) mouse model of Sjogren′s syndrome.

Materials and MethodsExpression of adiponectin and its receptors (AdipoR1/2) was detected by PCR, immunoblotting, or immunofluorescence. The level of adiponectin was quantified by ELISA. Adiponectin-related signaling molecules and pro-inflammatory cytokines were examined by PCR or immunoblotting. Apoptosis was evaluated by TUNEL staining, flow cytometry, and caspase 3 activation.

ResultsAdiponectin and AdipoR1/2 mRNA and protein were expressed in submandibular glands. Adiponectin immunostaining was widely diffused in the cytoplasm of acinar and ductal cells. AdipoR1 was mainly distributed in acinar cytoplasm, while AdipoR2 was predominantly located at acinar cell membrane. Submandibular adiponectin levels were reduced during the progression of autoimmune sialoadenitis in 7-, 14-, and 21-week-old NOD mice, while AdipoR1/2 levels were unchanged. The levels of phosphorylated adenosine monophosphate-activated protein kinase, extracellular signal-regulated kinase 1/2, and p38 mitogen-activated protein kinase were decreased, while interferon (IFN)- and glandular apoptosis were temporally increased at all time points. Moreover, exogenous adiponectin supplement inhibited, whereas neutralizing endogenous adiponectin by its antibody promoted IFN--induced apoptosis and caspase 3 activation in cultured submandibular acinar cells.

ConclusionsAdiponectin plays a protective role on submandibular cells. Decreased adiponectin might promote glandular destruction in autoimmune sialoadenitis.

语种: 英语
所属项目编号: 30973316 ; 81070847
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000344395800004
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50339
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Hlth Sci Ctr, Ctr Salivary Gland Dis,Sch & Hosp Stomatol,Minist, Beijing 100191, Peoples R China
2.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100191, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100191, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral Med & Tradit Chinese Med, Beijing 100191, Peoples R China

Recommended Citation:
Su, Y-C,Xiang, R-L,Zhang, Y.,et al. Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice[J]. ORAL DISEASES,2014,20(8):744-755.
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