|Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice|
|Su, Y-C1; Xiang, R-L1; Zhang, Y.1; Ding, C.2; Cong, X.1; Guo, X-H1; Yang, N-Y3; Hua, H.4; Wu, L-L1; Yu, G-Y3|
|关键词||Adiponectin Autoimmune Sialoadenitis Non-obese Diabetic Mice Sjogren&Prime s Syndrome|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||ACTIVATED PROTEIN-KINASE ; NF-KAPPA-B ; SJOGRENS-SYNDROME ; PLASMA ADIPONECTIN ; SALIVARY-GLAND ; INSULIN-RESISTANCE ; RHEUMATOID-ARTHRITIS ; EPITHELIAL-CELLS ; ADULT-RAT ; TNF-ALPHA|
ObjectiveTo investigate a possible role of adiponectin in the pathogenesis of autoimmune sialoadenitis in non-obese diabetic (NOD) mouse model of Sjogren′s syndrome.
Materials and MethodsExpression of adiponectin and its receptors (AdipoR1/2) was detected by PCR, immunoblotting, or immunofluorescence. The level of adiponectin was quantified by ELISA. Adiponectin-related signaling molecules and pro-inflammatory cytokines were examined by PCR or immunoblotting. Apoptosis was evaluated by TUNEL staining, flow cytometry, and caspase 3 activation.
ResultsAdiponectin and AdipoR1/2 mRNA and protein were expressed in submandibular glands. Adiponectin immunostaining was widely diffused in the cytoplasm of acinar and ductal cells. AdipoR1 was mainly distributed in acinar cytoplasm, while AdipoR2 was predominantly located at acinar cell membrane. Submandibular adiponectin levels were reduced during the progression of autoimmune sialoadenitis in 7-, 14-, and 21-week-old NOD mice, while AdipoR1/2 levels were unchanged. The levels of phosphorylated adenosine monophosphate-activated protein kinase, extracellular signal-regulated kinase 1/2, and p38 mitogen-activated protein kinase were decreased, while interferon (IFN)- and glandular apoptosis were temporally increased at all time points. Moreover, exogenous adiponectin supplement inhibited, whereas neutralizing endogenous adiponectin by its antibody promoted IFN--induced apoptosis and caspase 3 activation in cultured submandibular acinar cells.
ConclusionsAdiponectin plays a protective role on submandibular cells. Decreased adiponectin might promote glandular destruction in autoimmune sialoadenitis.
|项目编号||30973316 ; 81070847|
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Hlth Sci Ctr, Ctr Salivary Gland Dis,Sch & Hosp Stomatol,Minist, Beijing 100191, Peoples R China|
2.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100191, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100191, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral Med & Tradit Chinese Med, Beijing 100191, Peoples R China
|Su, Y-C,Xiang, R-L,Zhang, Y.,et al. Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice[J]. ORAL DISEASES,2014,20(8):744-755.|
|APA||Su, Y-C.,Xiang, R-L.,Zhang, Y..,Ding, C..,Cong, X..,...&Yu, G-Y.(2014).Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice.ORAL DISEASES,20(8),744-755.|
|MLA||Su, Y-C,et al."Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice".ORAL DISEASES 20.8(2014):744-755.|
|Decreased submandibu（2683KB）||期刊论文||出版稿||开放获取||CC BY-NC-SA||浏览 请求全文|