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Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice
Su, Y-C1; Xiang, R-L1; Zhang, Y.1; Ding, C.2; Cong, X.1; Guo, X-H1; Yang, N-Y3; Hua, H.4; Wu, L-L1; Yu, G-Y3
关键词Adiponectin Autoimmune Sialoadenitis Non-obese Diabetic Mice Sjogren&Prime s Syndrome
刊名ORAL DISEASES
2014-11-01
DOI10.1111/odi.12197
20期:8页:744-755
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]ACTIVATED PROTEIN-KINASE ; NF-KAPPA-B ; SJOGRENS-SYNDROME ; PLASMA ADIPONECTIN ; SALIVARY-GLAND ; INSULIN-RESISTANCE ; RHEUMATOID-ARTHRITIS ; EPITHELIAL-CELLS ; ADULT-RAT ; TNF-ALPHA
英文摘要

ObjectiveTo investigate a possible role of adiponectin in the pathogenesis of autoimmune sialoadenitis in non-obese diabetic (NOD) mouse model of Sjogren′s syndrome.

Materials and MethodsExpression of adiponectin and its receptors (AdipoR1/2) was detected by PCR, immunoblotting, or immunofluorescence. The level of adiponectin was quantified by ELISA. Adiponectin-related signaling molecules and pro-inflammatory cytokines were examined by PCR or immunoblotting. Apoptosis was evaluated by TUNEL staining, flow cytometry, and caspase 3 activation.

ResultsAdiponectin and AdipoR1/2 mRNA and protein were expressed in submandibular glands. Adiponectin immunostaining was widely diffused in the cytoplasm of acinar and ductal cells. AdipoR1 was mainly distributed in acinar cytoplasm, while AdipoR2 was predominantly located at acinar cell membrane. Submandibular adiponectin levels were reduced during the progression of autoimmune sialoadenitis in 7-, 14-, and 21-week-old NOD mice, while AdipoR1/2 levels were unchanged. The levels of phosphorylated adenosine monophosphate-activated protein kinase, extracellular signal-regulated kinase 1/2, and p38 mitogen-activated protein kinase were decreased, while interferon (IFN)- and glandular apoptosis were temporally increased at all time points. Moreover, exogenous adiponectin supplement inhibited, whereas neutralizing endogenous adiponectin by its antibody promoted IFN--induced apoptosis and caspase 3 activation in cultured submandibular acinar cells.

ConclusionsAdiponectin plays a protective role on submandibular cells. Decreased adiponectin might promote glandular destruction in autoimmune sialoadenitis.

语种英语
WOS记录号WOS:000344395800004
项目编号30973316 ; 81070847
资助机构National Natural Science Foundation of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50339
专题北京大学口腔医学院
北京大学基础医学院
北京大学口腔医学院_口腔黏膜科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Hlth Sci Ctr, Ctr Salivary Gland Dis,Sch & Hosp Stomatol,Minist, Beijing 100191, Peoples R China
2.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100191, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100191, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral Med & Tradit Chinese Med, Beijing 100191, Peoples R China
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Su, Y-C,Xiang, R-L,Zhang, Y.,et al. Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice[J]. ORAL DISEASES,2014,20(8):744-755.
APA Su, Y-C.,Xiang, R-L.,Zhang, Y..,Ding, C..,Cong, X..,...&Yu, G-Y.(2014).Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice.ORAL DISEASES,20(8),744-755.
MLA Su, Y-C,et al."Decreased submandibular adiponectin is involved in the progression of autoimmune sialoadenitis in non-obese diabetic mice".ORAL DISEASES 20.8(2014):744-755.
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