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学科主题临床医学
Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy
Meng, L. Q.1; Tang, J. W.1; Wang, Y.1; Zhao, J. R.1; Shang, M. Y.4; Zhang, M.1; Liu, S. Y.1; Qu, L.1; Cai, S. Q.4; Li, X. M.1
关键词Astragaloside Iv Ferulic Acid Renal Tubulointerstitial Fibrosis Mitogen-activated Protein Kinase Unilateral Ureteral Obstruction Rat Astragali Radix Angelicae Sinensis Radix
刊名BRITISH JOURNAL OF PHARMACOLOGY
2011-04-01
DOI10.1111/j.1476-5381.2011.01206.x
162期:8页:1805-1818
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]CHRONIC KIDNEY-DISEASE ; ACTIVATED PROTEIN-KINASE ; EPITHELIAL-MYOFIBROBLAST TRANSDIFFERENTIATION ; MEMBRANACEUS VAR. MONGHOLICUS ; TO-MESENCHYMAL TRANSITION ; BETA SIGNAL-TRANSDUCTION ; CHINESE HERBS ; ANGELICA-SINENSIS ; ANGIOTENSIN-II ; NITRIC-OXIDE
英文摘要

BACKGROUND AND PURPOSE

The combination of Chinese herbs, Astragali Radix and Angelicae Sinensis Radix, could alleviate renal interstitial fibrosis. Astragaloside IV (AS-IV) and ferulic acid (FA) are the two major active constituents in this combination. In this study, we employed rats with unilateral ureteral obstruction to determine whether AS-IV and FA have the same renoprotective effects and investigated the mechanisms of this action.

EXPERIMENTAL APPROACH

Renal pathological changes were evaluated after treatment with AS-IV, FA or AS-IV + FA (AF) for 10 days. Meanwhile, the expression of transforming growth factor beta(1) (TGF-beta(1)), fibronectin, alpha-smooth muscle actin (alpha-SMA), phosphorylation of c-Jun NH2-terminal kinase (p-JNK) and nitric oxide (NO) production in kidney were determined. The expressions of fibronectin, a-SMA, mitogen-activated protein kinases [JNK, extracellular signal-regulated kinases (ERK), P38] in TGF-beta 1-treated NRK-49F cells or interleukin-1-treated HK-2 cells after AS-IV, FA or AF were assessed.

KEY RESULTS

AF alleviated the infiltration of mononuclear cells, tubular atrophy and interstitial fibrosis; reduced the expression of fibronectin, alpha-SMA, TGF-beta(1) and p-JNK; and dramatically increased the production of NO in obstructed kidneys. Neither AS-IV nor FA alone improved renal damage, but both increased NO production. AF inhibited a-SMA and fibronectin expression in NRK-49F or HK-2 cells. Furthermore, AF significantly inhibited IL-1 beta-induced JNK phosphorylation, without affecting ERK or P38 phosphorylation. Neither AS-IV nor FA alone had any effect on the cells.

CONCLUSIONS AND IMPLICATIONS

AS-IV synergizes with FA to alleviate renal tubulointerstitial fibrosis; this was associated with inhibition of tubular epithelial-mesenchymal transdifferentiation (EMT) and fibroblast activation, as well as an increase in NO production in the kidney.

语种英语
WOS记录号WOS:000288704500012
项目编号30330710 ; 20070001761
资助机构National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50340
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Div Renal, Dept Med, Hosp 1, Beijing 100871, Peoples R China
2.Peking Univ, Inst Nephrol, Beijing 100871, Peoples R China
3.Minist Hlth China, Key Lab Renal Dis, Beijing, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Meng, L. Q.,Tang, J. W.,Wang, Y.,et al. Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy[J]. BRITISH JOURNAL OF PHARMACOLOGY,2011,162(8):1805-1818.
APA Meng, L. Q..,Tang, J. W..,Wang, Y..,Zhao, J. R..,Shang, M. Y..,...&Li, X. M..(2011).Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy.BRITISH JOURNAL OF PHARMACOLOGY,162(8),1805-1818.
MLA Meng, L. Q.,et al."Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy".BRITISH JOURNAL OF PHARMACOLOGY 162.8(2011):1805-1818.
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