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学科主题: 基础医学
题名:
Kissing and nanotunneling mediate intermitochondrial communication in the heart
作者: Huang, Xiaohu1; Sun, Lei3; Ji, Shuangxi2; Zhao, Ting1; Zhang, Wanrui1; Xu, Jiejia1; Zhang, Jue2; Wang, Yanru1; Wang, Xianhua1; Franzini-Armstrong, Clara4; Zheng, Ming1,5; Cheng, Heping1
关键词: mitochondrial dynamics ; nanotubule
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
发表日期: 2013-02-19
DOI: 10.1073/pnas.1300741110
卷: 110, 期:8, 页:2846-2851
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MITOCHONDRIAL FUSION ; EMBRYONIC-DEVELOPMENT ; ELECTRON-MICROSCOPY ; PLASTID MORPHOLOGY ; SKELETAL-MUSCLE ; DYNAMICS ; FISSION ; CARDIOMYOCYTES ; MITOFUSINS ; STROMULES
英文摘要:

Mitochondria in many types of cells are dynamically interconnected through constant fusion and fission, allowing for exchange of mitochondrial contents and repair of damaged mitochondria. However, constrained by the myofibril lattice, the similar to 6,000 mitochondria in the adult mammalian cardiomyocyte display little motility, and it is unclear how, if at all, they communicate with each other. By means of target-expressing photoactivatable green fluorescent protein (PAGFP) in the mitochondrial matrix or on the outer mitochondrial membrane, we demonstrated that the local PAGFP signal propagated over the entire population of mitochondria in cardiomyocytes on a time scale of similar to 10 h. Two elemental steps of intermitochondrial communications were manifested as either a sudden PAGFP transfer between a pair of adjacent mitochondria (i.e., "kissing") or a dynamic nanotubular tunnel (i.e., "nanotunneling") between nonadjacent mitochondria. The average content transfer index (fractional exchange) was around 0.5; the rate of kissing was 1% s(-1) per mitochondrial pair, and that of nanotunneling was about 14 times smaller. Electron microscopy revealed extensive intimate contacts between adjacent mitochondria and elongated nanotubular protrusions, providing a structural basis for the kissing and nanotunneling, respectively. We propose that, through kissing and nanotunneling, the otherwise static mitochondria in a cardiomyocyte form one dynamically continuous network to share content and transfer signals.

语种: 英语
所属项目编号: 2013CB531203 ; 2011CB809102 ; 30971062 ; 31130067 ; 31221002 ; R01 HL 48093
项目资助者: 973 Program ; National Science Foundation of China ; National Institutes of Health
WOS记录号: WOS:000315954400054
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50348
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Inst Mol Med, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Ctr Biol Imaging, Beijing 100101, Peoples R China
4.Univ Penn, Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
5.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Huang, Xiaohu,Sun, Lei,Ji, Shuangxi,et al. Kissing and nanotunneling mediate intermitochondrial communication in the heart[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(8):2846-2851.
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