IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas
Wang, Zhaohui1,2; Li, Lili1,2; Su, Xianwei2; Gao, Zifen3; Srivastava, Gopesh4; Murray, Paul G.5; Ambinder, Richard6,7; Tao, Qian1,2,6,7
关键词DLEC1 CpG Methylation Tumor suppressor Lymphoma
刊名JOURNAL OF TRANSLATIONAL MEDICINE
2012-10-11
DOI10.1186/1479-5876-10-209
10期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]HUMAN-CHROMOSOME 3P21.3 ; HOMOZYGOUS DELETION REGION ; NASOPHARYNGEAL CARCINOMA ; LUNG-CANCER ; CLINICOPATHOLOGICAL SIGNIFICANCE ; ABERRANT METHYLATION ; DNA METHYLATION ; UTERINE CERVIX ; CELL CARCINOMA ; HIGH-FREQUENCY
英文摘要

Background: Inactivaion of tumor suppressor genes (TSGs) by promoter CpG methylation frequently occurs in tumorigenesis, even in the early stages, contributing to the initiation and progression of human cancers. Deleted in lung and esophageal cancer 1 (DLEC1), located at the 3p22-21.3 TSG cluster, has been identified frequently silenced by promoter CpG methylation in multiple carcinomas, however, no study has been performed for lymphomas yet.

Methods: We examined the expression of DLEC1 by semi-quantitative reverse transcription (RT)-PCR, and evaluated the promoter methylation of DLEC1 by methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) in common lymphoma cell lines and tumors.

Results: Here we report that DLEC1 is readily expressed in normal lymphoid tissues including lymph nodes and PBMCs, but reduced or silenced in 70% (16/23) of non-Hodgkin and Hodgkin lymphoma cell lines, including 2/6 diffuse large B-cell (DLBCL), 1/2 peripheral T cell lymphomas, 5/5 Burkitt, 6/7 Hodgkin and 2/3 nasal killer (NK)/T-cell lymphoma cell lines. Promoter CpG methylation was frequently detected in 80% (20/25) of lymphoma cell lines and correlated with DLEC1 downregulation/silencing. Pharmacologic demethylation reversed DLEC1 expression in lymphoma cell lines along with concomitant promoter demethylation. DLEC1 methylation was also frequently detected in 32 out of 58 (55%) different types of lymphoma tissues, but not in normal lymph nodes. Furthermore, DLEC1 was specifically methylated in the sera of 3/13 (23%) Hodgkin lymphoma patients.

Conclusions: Thus, methylation-mediated silencing of DLEC1 plays an important role in multiple lymphomagenesis, and may serve as a non-invasive tumor marker for lymphoma diagnosis.

语种英语
WOS记录号WOS:000313638300001
项目编号81071634 ; 81172582 ; JC201005270328A
资助机构National Natural Science Foundation of China ; Shenzhen Science Fund for Distinguished Young Scholars ; Leukaemia Lymphoma Research of the United Kingdom ; Chinese University of Hong Kong
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50358
专题北京大学基础医学院_病理学系
北京大学基础医学院
作者单位1.Chinese Acad Sci, CUHK, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
2.Chinese Univ Hong Kong, Sir YK Pao Ctr Canc, State Key Lab Oncol S China, Canc Epigenet Lab,Dept Clin Oncol, Shatin, Hong Kong, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100871, Peoples R China
4.Univ Hong Kong, Dept Pathol, Shatin, Hong Kong, Peoples R China
5.Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
6.Johns Hopkins Singapore, Baltimore, MD USA
7.Johns Hopkins Sch Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA
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Wang, Zhaohui,Li, Lili,Su, Xianwei,et al. Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2012,10(1).
APA Wang, Zhaohui.,Li, Lili.,Su, Xianwei.,Gao, Zifen.,Srivastava, Gopesh.,...&Tao, Qian.(2012).Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas.JOURNAL OF TRANSLATIONAL MEDICINE,10(1).
MLA Wang, Zhaohui,et al."Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas".JOURNAL OF TRANSLATIONAL MEDICINE 10.1(2012).
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