北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病理学系  > 期刊论文
学科主题: 基础医学
题名:
Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas
作者: Wang, Zhaohui1,2; Li, Lili1,2; Su, Xianwei2; Gao, Zifen3; Srivastava, Gopesh4; Murray, Paul G.5; Ambinder, Richard6,7; Tao, Qian1,2,6,7
关键词: DLEC1 ; CpG ; Methylation ; Tumor suppressor ; Lymphoma
刊名: JOURNAL OF TRANSLATIONAL MEDICINE
发表日期: 2012-10-11
DOI: 10.1186/1479-5876-10-209
卷: 10, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: HUMAN-CHROMOSOME 3P21.3 ; HOMOZYGOUS DELETION REGION ; NASOPHARYNGEAL CARCINOMA ; LUNG-CANCER ; CLINICOPATHOLOGICAL SIGNIFICANCE ; ABERRANT METHYLATION ; DNA METHYLATION ; UTERINE CERVIX ; CELL CARCINOMA ; HIGH-FREQUENCY
英文摘要:

Background: Inactivaion of tumor suppressor genes (TSGs) by promoter CpG methylation frequently occurs in tumorigenesis, even in the early stages, contributing to the initiation and progression of human cancers. Deleted in lung and esophageal cancer 1 (DLEC1), located at the 3p22-21.3 TSG cluster, has been identified frequently silenced by promoter CpG methylation in multiple carcinomas, however, no study has been performed for lymphomas yet.

Methods: We examined the expression of DLEC1 by semi-quantitative reverse transcription (RT)-PCR, and evaluated the promoter methylation of DLEC1 by methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) in common lymphoma cell lines and tumors.

Results: Here we report that DLEC1 is readily expressed in normal lymphoid tissues including lymph nodes and PBMCs, but reduced or silenced in 70% (16/23) of non-Hodgkin and Hodgkin lymphoma cell lines, including 2/6 diffuse large B-cell (DLBCL), 1/2 peripheral T cell lymphomas, 5/5 Burkitt, 6/7 Hodgkin and 2/3 nasal killer (NK)/T-cell lymphoma cell lines. Promoter CpG methylation was frequently detected in 80% (20/25) of lymphoma cell lines and correlated with DLEC1 downregulation/silencing. Pharmacologic demethylation reversed DLEC1 expression in lymphoma cell lines along with concomitant promoter demethylation. DLEC1 methylation was also frequently detected in 32 out of 58 (55%) different types of lymphoma tissues, but not in normal lymph nodes. Furthermore, DLEC1 was specifically methylated in the sera of 3/13 (23%) Hodgkin lymphoma patients.

Conclusions: Thus, methylation-mediated silencing of DLEC1 plays an important role in multiple lymphomagenesis, and may serve as a non-invasive tumor marker for lymphoma diagnosis.

语种: 英语
所属项目编号: 81071634 ; 81172582 ; JC201005270328A
项目资助者: National Natural Science Foundation of China ; Shenzhen Science Fund for Distinguished Young Scholars ; Leukaemia Lymphoma Research of the United Kingdom ; Chinese University of Hong Kong
WOS记录号: WOS:000313638300001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50358
Appears in Collections:基础医学院_病理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas.pdf(1087KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Chinese Acad Sci, CUHK, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
2.Chinese Univ Hong Kong, Sir YK Pao Ctr Canc, State Key Lab Oncol S China, Canc Epigenet Lab,Dept Clin Oncol, Shatin, Hong Kong, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100871, Peoples R China
4.Univ Hong Kong, Dept Pathol, Shatin, Hong Kong, Peoples R China
5.Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
6.Johns Hopkins Singapore, Baltimore, MD USA
7.Johns Hopkins Sch Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA

Recommended Citation:
Wang, Zhaohui,Li, Lili,Su, Xianwei,et al. Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2012,10(1).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Zhaohui]'s Articles
[Li, Lili]'s Articles
[Su, Xianwei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Zhaohui]‘s Articles
[Li, Lili]‘s Articles
[Su, Xianwei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace