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学科主题临床医学
Effects of oocyte vitrification on histone modifications
Yan, Li-Ying; Yan, Jie; Qiao, Jie; Zhao, Pan-Lin; Liu, Ping
关键词Cryopreservation Mouse
刊名REPRODUCTION FERTILITY AND DEVELOPMENT
2010
DOI10.1071/RD09312
22期:6页:920-925
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Reproductive Biology ; Zoology
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Fundamental Research Funds for the Central Universities ; Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period ; Major State Basic Research Development Program of the People&prime ; s Republic of China ; Fundamental Research Funds for the Central Universities ; Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period
研究领域[WOS]Developmental Biology ; Reproductive Biology ; Zoology
关键词[WOS]DNA METHYLATION ; ZONA-PELLUCIDA ; MOUSE OOCYTES ; IN-VITRO ; CRYOPRESERVATION ; FERTILIZATION ; PLURIPOTENCY ; CHROMOSOME ; SPINDLE ; EMBRYO
英文摘要

Vitrification has been widely used as an assisted reproductive technology in animals and humans, yet the impact of oocyte vitrification and warming on survival and histone modifications has to be evaluated. In the present study, the survival of mouse MII oocytes was assessed after freezing, as were changes in histone 3 lysine 9 (H3K9) dimethylation, histone 4 lysine 5 (H4K5) acetylation and histone 3 lysine 14 (H3K14) acetylation. The results show that, in oocytes subjected to vitrification, H3K9 methylation and H4K5 acetylation were increased. H3K14 acetylation could not be detected in either non-vitrified or vitrified oocytes. Oocytes are very sensitive to changes in H3K9 and H4K5 following vitrification. Both these histone modifications could be useful markers to monitor epigenetic perturbations induced by various experimental vitrification protocols and eventually for optimising the cryopreservation of human oocytes.

语种英语
所属项目编号2007CB948102 ; BMU20090514-603 ; 2007BA104B00
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Fundamental Research Funds for the Central Universities ; Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period ; Major State Basic Research Development Program of the People&prime ; s Republic of China ; Fundamental Research Funds for the Central Universities ; Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period
WOS记录号WOS:000279384100004
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50362
专题北京大学第三临床医学院_生殖医学中心
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yan, Li-Ying,Yan, Jie,Qiao, Jie,et al. Effects of oocyte vitrification on histone modifications[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2010,22(6):920-925.
APA Yan, Li-Ying,Yan, Jie,Qiao, Jie,Zhao, Pan-Lin,&Liu, Ping.(2010).Effects of oocyte vitrification on histone modifications.REPRODUCTION FERTILITY AND DEVELOPMENT,22(6),920-925.
MLA Yan, Li-Ying,et al."Effects of oocyte vitrification on histone modifications".REPRODUCTION FERTILITY AND DEVELOPMENT 22.6(2010):920-925.
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