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学科主题: 临床医学
题名:
Effects of oocyte vitrification on histone modifications
作者: Yan, Li-Ying; Yan, Jie; Qiao, Jie; Zhao, Pan-Lin; Liu, Ping
关键词: cryopreservation ; mouse
刊名: REPRODUCTION FERTILITY AND DEVELOPMENT
发表日期: 2010
DOI: 10.1071/RD09312
卷: 22, 期:6, 页:920-925
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology ; Zoology
研究领域[WOS]: Developmental Biology ; Reproductive Biology ; Zoology
关键词[WOS]: DNA METHYLATION ; ZONA-PELLUCIDA ; MOUSE OOCYTES ; IN-VITRO ; CRYOPRESERVATION ; FERTILIZATION ; PLURIPOTENCY ; CHROMOSOME ; SPINDLE ; EMBRYO
英文摘要:

Vitrification has been widely used as an assisted reproductive technology in animals and humans, yet the impact of oocyte vitrification and warming on survival and histone modifications has to be evaluated. In the present study, the survival of mouse MII oocytes was assessed after freezing, as were changes in histone 3 lysine 9 (H3K9) dimethylation, histone 4 lysine 5 (H4K5) acetylation and histone 3 lysine 14 (H3K14) acetylation. The results show that, in oocytes subjected to vitrification, H3K9 methylation and H4K5 acetylation were increased. H3K14 acetylation could not be detected in either non-vitrified or vitrified oocytes. Oocytes are very sensitive to changes in H3K9 and H4K5 following vitrification. Both these histone modifications could be useful markers to monitor epigenetic perturbations induced by various experimental vitrification protocols and eventually for optimising the cryopreservation of human oocytes.

语种: 英语
所属项目编号: 2007CB948102 ; BMU20090514-603 ; 2007BA104B00
项目资助者: Major State Basic Research Development Program of the People&prime ; s Republic of China ; Fundamental Research Funds for the Central Universities ; Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period
WOS记录号: WOS:000279384100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50362
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China

Recommended Citation:
Yan, Li-Ying,Yan, Jie,Qiao, Jie,et al. Effects of oocyte vitrification on histone modifications[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2010,22(6):920-925.
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