IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers
Najafzadeh, M. J.1,2,3; Dolatabadi, S.3; Keisari, M. Saradeghi3,4; Naseri, A.5; Feng, P.3,6; de Hoog, G. S.3,7,8,9,10,11,12
关键词Exophiala Black Yeasts Identification Rolling Circle Amplification
刊名JOURNAL OF MICROBIOLOGICAL METHODS
2013-09-01
DOI10.1016/j.mimet.2013.06.026
94期:3页:338-342
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]IN-VITRO ACTIVITIES ; RAPID IDENTIFICATION ; ANTIFUNGAL DRUGS ; HUMAN CHROMOBLASTOMYCOSIS ; FUNGAL PATHOGENS ; UNITED-STATES ; DERMATITIDIS ; FONSECAEA ; AGENT ; CYPHELLOPHORA
英文摘要

Deep infections by melanized fungi deserve special attention because of a potentially fatal, cerebral or disseminated course of disease in otherwise healthy patients. Timely diagnostics are a major problem with these infections. Rolling circle amplification (RCA) is a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of microorganisms. RCA-based diagnostics are characterized by good reproducibility, with few amplification errors compared to PCR. The method is applied here to species of Exophiala known to cause systemic infections in humans. The ITS rDNA region of five Exophiala species (E. dermatitidis, E. oligosperma, E. spinifera, E. xenobiotica, and E. jeanselmei) was sequenced and aligned in view of designing specific padlock probes to be used for the detection of single nucleotide polymorphisms (SNPs) of the Exophiala species concerned. The assay proved to successfully amplify DNA of the target fungi at the level of species; while no cross-reactivity was observed. Amplification products were visualized on 1% agarose gels to verify the specificity of probe-template binding. Amounts of reagents were minimized to avoid the generation of false positive results. The sensitivity of RCA may help to improve early diagnostics of these difficult to diagnose infections. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000324664500028
项目编号920110
资助机构Mashhad University of Medical Sciences, Mashhad, Iran
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50365
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
2.Mashhad Univ Med Sci, Sch Med, Ghaem Hosp, Dept Parasitol & Mycol, Mashhad, Iran
3.Mashhad Univ Med Sci, Sch Med, Ghaem Hosp, Canc Mol Pathol Res Ctr, Mashhad, Iran
4.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
5.Mashhad Univ Med Sci, Food & Drug Adm, Mashhad, Iran
6.Mashhad Univ Med Sci, Sch Med, Imam Reza Hosp, Dept Parasitol & Mycol, Mashhad, Iran
7.Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou 510275, Guangdong, Peoples R China
8.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
9.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
10.Second Mil Med Univ, Changzheng Hosp, Shanghai Inst Med Mycol, Shanghai, Peoples R China
11.Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil
12.King Abdulassiz Univ, Jeddah, Saudi Arabia
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Najafzadeh, M. J.,Dolatabadi, S.,Keisari, M. Saradeghi,et al. Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2013,94(3):338-342.
APA Najafzadeh, M. J.,Dolatabadi, S.,Keisari, M. Saradeghi,Naseri, A.,Feng, P.,&de Hoog, G. S..(2013).Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers.JOURNAL OF MICROBIOLOGICAL METHODS,94(3),338-342.
MLA Najafzadeh, M. J.,et al."Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers".JOURNAL OF MICROBIOLOGICAL METHODS 94.3(2013):338-342.
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