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学科主题: 临床医学
题名:
The experimental study of ovarian carcinoma vaccine modified by human B7-1 and IFN-gamma genes
作者: Qian, HN; Liu, GZ; Cao, SJ; Feng, J; Ye, X
关键词: B7-1(CD80) ; IFN-gamma ; immunotherapy ; ovarian carcinoma vaccine ; retroviral vector
刊名: INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
发表日期: 2002
卷: 12, 期:1, 页:80-85
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: TUMOR-CELLS ; INTERFERON-GAMMA ; POTENT ; MICE
英文摘要:

With the advance of immunology, immunogene therapy is becoming a possible therapeutic alternative to advanced cancer. The aim of tumor immunogene therapy is to enhance the immune response to tumors. Evidence suggests that CD80 (B7-1) and Interferon-gamma (IFN-gamma) play important roles in antitumor immunity induced by T lymphocyte. To study the antitumor immune effects of ovarian carcinoma vaccine modified with human B7-1 and IFN-gamma genes, we constructed the bicistronic retroviral vector pLXSN, encoding human B7-1 and IFN-gamma. In vitro, the primary ovarian carcinoma cells were transduced with the retroviral vector and prepared as a vaccine. Then autologous lymphocytes were irritated with the ovarian carcinoma cells for competition inhibitory cytotoxic testing. The tumor-specific cytotoxic activity was greatly enhanced by the double gene-modified vaccine. In vivo, the tumorigenicity of the double gene-modified ovarian cell line 3AO/B7-1.IFN-gamma was evaluated in a human immune reconstituted SCID mice (hu-PBL-SCID) model. The double-gene modification markedly decreases tumor genecity. Together, the results suggest that combining B7-1 and IFN-gamma could be a useful therapeutic approach in ovarian cancer.

语种: 英语
WOS记录号: WOS:000174059600012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50380
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Qian, HN,Liu, GZ,Cao, SJ,et al. The experimental study of ovarian carcinoma vaccine modified by human B7-1 and IFN-gamma genes[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2002,12(1):80-85.
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