北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
The experimental study of ovarian carcinoma vaccine modified by human B7-1 and IFN-gamma genes
作者: Qian, HN; Liu, GZ; Cao, SJ; Feng, J; Ye, X
关键词: B7-1(CD80) ; IFN-gamma ; immunotherapy ; ovarian carcinoma vaccine ; retroviral vector
刊名: INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
发表日期: 2002
卷: 12, 期:1, 页:80-85
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: TUMOR-CELLS ; INTERFERON-GAMMA ; POTENT ; MICE
英文摘要:

With the advance of immunology, immunogene therapy is becoming a possible therapeutic alternative to advanced cancer. The aim of tumor immunogene therapy is to enhance the immune response to tumors. Evidence suggests that CD80 (B7-1) and Interferon-gamma (IFN-gamma) play important roles in antitumor immunity induced by T lymphocyte. To study the antitumor immune effects of ovarian carcinoma vaccine modified with human B7-1 and IFN-gamma genes, we constructed the bicistronic retroviral vector pLXSN, encoding human B7-1 and IFN-gamma. In vitro, the primary ovarian carcinoma cells were transduced with the retroviral vector and prepared as a vaccine. Then autologous lymphocytes were irritated with the ovarian carcinoma cells for competition inhibitory cytotoxic testing. The tumor-specific cytotoxic activity was greatly enhanced by the double gene-modified vaccine. In vivo, the tumorigenicity of the double gene-modified ovarian cell line 3AO/B7-1.IFN-gamma was evaluated in a human immune reconstituted SCID mice (hu-PBL-SCID) model. The double-gene modification markedly decreases tumor genecity. Together, the results suggest that combining B7-1 and IFN-gamma could be a useful therapeutic approach in ovarian cancer.

语种: 英语
WOS记录号: WOS:000174059600012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50380
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Qian, HN,Liu, GZ,Cao, SJ,et al. The experimental study of ovarian carcinoma vaccine modified by human B7-1 and IFN-gamma genes[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2002,12(1):80-85.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qian, HN]'s Articles
[Liu, GZ]'s Articles
[Cao, SJ]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qian, HN]‘s Articles
[Liu, GZ]‘s Articles
[Cao, SJ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace