IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells
Yang, HX; Zhang, JZ; Wu, J; Shou, CC
刊名HYBRIDOMA
2005-02-01
24期:1页:36-41
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
英文摘要

Nucleostemin (NS) is a novel protein that promotes the proliferation of stem cells and cancer cells. The aim of this study is to prepare specific monoclonal antibodies against nucleostemin as a tool in nucleostemin research. To generate the antibodies, the cDNA coding nucleostemin was cloned from human gastric cancer cell line NCI-N87 with reverse transcriptase-polymerase chain reaction (RT-PCR). The partial cDNA fragment was inserted into prokaryotic expression vector pGEX-4T1 to make glutathione S-transferase (GST) fusion protein in Escherichia coli, and the whole-length cDNA fragment was cloned into vector pcDNA3 for expression in mammalian cells. The spleen cells, from the BALB/c mouse immunized with the GST-nucleostemin fusion protein, were used to prepare the monoclonal antibody with hybridoma technique. After screening with enzyme-linked immunosorbent assay (ELISA) and subcloning, six specific monoclonal antibodies against nucleostemin were obtained. Moreover, they also worked well in Western blotting, immunoprecipitation, and immunohistochemistry. These antibodies will be helpful in elucidating biological functions and the molecular mechanism of nucleostemin.

语种英语
WOS记录号WOS:000228474200005
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50381
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_胸部肿瘤外二科
北京大学临床肿瘤学院_生化与分子生物学研究室
作者单位Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Yang, HX,Zhang, JZ,Wu, J,et al. Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells[J]. HYBRIDOMA,2005,24(1):36-41.
APA Yang, HX,Zhang, JZ,Wu, J,&Shou, CC.(2005).Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells.HYBRIDOMA,24(1),36-41.
MLA Yang, HX,et al."Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells".HYBRIDOMA 24.1(2005):36-41.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, HX]的文章
[Zhang, JZ]的文章
[Wu, J]的文章
百度学术
百度学术中相似的文章
[Yang, HX]的文章
[Zhang, JZ]的文章
[Wu, J]的文章
必应学术
必应学术中相似的文章
[Yang, HX]的文章
[Zhang, JZ]的文章
[Wu, J]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。