IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells
Yang, HX; Zhang, JZ; Wu, J; Shou, CC
刊名HYBRIDOMA
2005-02-01
24期:1页:36-41
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
英文摘要

Nucleostemin (NS) is a novel protein that promotes the proliferation of stem cells and cancer cells. The aim of this study is to prepare specific monoclonal antibodies against nucleostemin as a tool in nucleostemin research. To generate the antibodies, the cDNA coding nucleostemin was cloned from human gastric cancer cell line NCI-N87 with reverse transcriptase-polymerase chain reaction (RT-PCR). The partial cDNA fragment was inserted into prokaryotic expression vector pGEX-4T1 to make glutathione S-transferase (GST) fusion protein in Escherichia coli, and the whole-length cDNA fragment was cloned into vector pcDNA3 for expression in mammalian cells. The spleen cells, from the BALB/c mouse immunized with the GST-nucleostemin fusion protein, were used to prepare the monoclonal antibody with hybridoma technique. After screening with enzyme-linked immunosorbent assay (ELISA) and subcloning, six specific monoclonal antibodies against nucleostemin were obtained. Moreover, they also worked well in Western blotting, immunoprecipitation, and immunohistochemistry. These antibodies will be helpful in elucidating biological functions and the molecular mechanism of nucleostemin.

语种英语
WOS记录号WOS:000228474200005
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50381
Collection北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Yang, HX,Zhang, JZ,Wu, J,et al. Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells[J]. HYBRIDOMA,2005,24(1):36-41.
APA Yang, HX,Zhang, JZ,Wu, J,&Shou, CC.(2005).Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells.HYBRIDOMA,24(1),36-41.
MLA Yang, HX,et al."Preparation and characterization of monoclonal antibodies against nucleostemin, a protein that controls cell proliferation in stem cells and cancer cells".HYBRIDOMA 24.1(2005):36-41.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yang, HX]'s Articles
[Zhang, JZ]'s Articles
[Wu, J]'s Articles
百度学术
百度学术Similar articles in
[Yang, HX]'s Articles
[Zhang, JZ]'s Articles
[Wu, J]'s Articles
必应学术
必应学术Similar articles in
[Yang, HX]'s Articles
[Zhang, JZ]'s Articles
[Wu, J]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.