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学科主题临床医学
Histone Deacetylase Inhibitor Trichostatin A Promotes the Osteogenic Differentiation of Rat Adipose-Derived Stem Cells by Altering the Epigenetic Modifications on Runx2 Promoter in a BMP Signaling-Dependent Manner
Hu, Xiaoqing1; Zhang, Xin1; Dai, Linghui1; Zhu, Jingxian1; Jia, Zhuqing2; Wang, Weiping2; Zhou, Chunyan2; Ao, Yingfang1
刊名STEM CELLS AND DEVELOPMENT
2013
DOI10.1089/scd.2012.0105
22期:2页:248-255
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
资助者Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Sciences Foundation of China ; 111 Project of China ; China Postdoctoral Science Foundation ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Sciences Foundation of China ; 111 Project of China ; China Postdoctoral Science Foundation
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]BONE-FORMATION ; IN-VITRO ; TISSUE ; EXPRESSION ; RUNX2/CBFA1 ; PHENOTYPE ; MARROW
英文摘要

Adult stem cells reside in many types of tissues and adult stem cell-based regenerative medicine holds great promise for repair of diseased tissues. Recently, adipose-derived stem cells (ADSCs) were found to be an appealing alternative to bone marrow stem cells (BMSCs) for tissue-engineered bone regeneration. Compared with BMSCs, ADSCs can be easily and abundantly available from adipose tissue. However, our previous study has discovered an important phenomenon that BMSCs have greater osteogenic potential than ADSCs in vitro. In this study, we aimed to explore its mechanism and improve the osteogenic potential of ADSCs for bone tissue regeneration. It has been reported that the epigenetic states could contribute to lineage-specific differentiation of adult stem cells. We observed that the epigenetic changes of BMSCs were much greater compared with ADSCs after a 3-day osteogenic induction. Runt-related transcription factor 2 (Runx2) is essential for osteoblast differentiation and bone formation. We found that BMSCs underwent more obvious epigenetic changes on the Runx2 promoter than ADSCs after osteogenic induction. These results suggest the epigenetic regulation involvement in Runx2 expression, and thus osteogenesis. We subsequently used a histone deacetylase inhibitor, trichostatin A (TSA), to promote the osteogenesis capacity of ADSCs. The results showed that TSA promoted rat ADSCs osteogenic differentiation by altering the epigenetic modifications on the Runx2 promoter in a bone morphogenetic protein signaling-dependent manner.

语种英语
所属项目编号20110001130001 ; 90919022 ; 81101350 ; 81070112 ; 81071675 ; B07001 ; 20110490249
资助者Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Sciences Foundation of China ; 111 Project of China ; China Postdoctoral Science Foundation ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Sciences Foundation of China ; 111 Project of China ; China Postdoctoral Science Foundation
WOS记录号WOS:000313577400007
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50391
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Inst Sports Med, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Hu, Xiaoqing,Zhang, Xin,Dai, Linghui,et al. Histone Deacetylase Inhibitor Trichostatin A Promotes the Osteogenic Differentiation of Rat Adipose-Derived Stem Cells by Altering the Epigenetic Modifications on Runx2 Promoter in a BMP Signaling-Dependent Manner[J]. STEM CELLS AND DEVELOPMENT,2013,22(2):248-255.
APA Hu, Xiaoqing.,Zhang, Xin.,Dai, Linghui.,Zhu, Jingxian.,Jia, Zhuqing.,...&Ao, Yingfang.(2013).Histone Deacetylase Inhibitor Trichostatin A Promotes the Osteogenic Differentiation of Rat Adipose-Derived Stem Cells by Altering the Epigenetic Modifications on Runx2 Promoter in a BMP Signaling-Dependent Manner.STEM CELLS AND DEVELOPMENT,22(2),248-255.
MLA Hu, Xiaoqing,et al."Histone Deacetylase Inhibitor Trichostatin A Promotes the Osteogenic Differentiation of Rat Adipose-Derived Stem Cells by Altering the Epigenetic Modifications on Runx2 Promoter in a BMP Signaling-Dependent Manner".STEM CELLS AND DEVELOPMENT 22.2(2013):248-255.
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