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Mechanism of ascorbic acid-induced reversion against malignant phenotype in human gastric cancer cells
Sun, Ya-Xuan1; Zheng, Qiu-Sheng1; Li, Gang1; Guo, De-An1; Wang, Zi-Ren1
关键词Aminotriazole Ascorbic Acid Catalase Gastric Cancer Hydrogen Peroxide Redifferentiation Superoxide Dismutase
刊名BIOMEDICAL AND ENVIRONMENTAL SCIENCES
2006-10-01
19期:5页:385-391
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]NEURO-BLASTOMA CELLS ; VITAMIN-C ; LIPID-PEROXIDATION ; HYDROGEN-PEROXIDE ; GROWTH ; PROOXIDANT ; INVITRO ; LIVER ; DIFFERENTIATION ; CATALASE
英文摘要

Objective To find out the mechanisms of redifferentiation and reversion of malignant human gastric cancer cells induced by ascorbic acid. Methods Human gastric cancer cells grown in the laboratory were used. The Trypan blue dye exclusion method was used to determine the cell doubling time. The electrophoresis rate and colonogenic potential were the indices used to measure the rate of redifferentiation. The content of malondialdehyde (MDA) was measured using the thiobarbituric acid (TBA) method. The activities of superoxide dismutase (SOD), catalase (CAT) and the content of H2O2 were evaluated by spectrophotography. Results Six mmol/L ascorbic acid was used as a positive control. Human gastric cancer cells were treated with 75 pm hydrogen peroxide, which alleviated many of the malignant characteristics. For example, the cell surface charge obviously decreased and the electrophoresis rate dropped from 2.21 to 1.10 gm(.)s(-1.)V(.)cm(-1). The colonogenic potential, a measure of cell differentiation, decreased 90.2%. After treatment with ascorbic acid, there was a concentration- and time-dependent increase in hydrogen peroxide (H2O2) and the activity of superoxide dismutase (SOD). However, the activity of catalase (CAT) resulted in a concentration- and time-dependent decrease. SOD and 3-amino-1,2,4-triazole (AT) exhibited some effects, but there were statistically significant differences between the SOD and AT group and the H2O2 group. Conclusions Ascorbic acid induces growth inhibition and redifferentiation of human gastric cancer cells through the production of hydrogen peroxide.

语种英语
WOS记录号WOS:000241586600011
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50400
专题北京大学药学院
作者单位1.Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Xinjiang 832002, Peoples R China
2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
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GB/T 7714
Sun, Ya-Xuan,Zheng, Qiu-Sheng,Li, Gang,et al. Mechanism of ascorbic acid-induced reversion against malignant phenotype in human gastric cancer cells[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2006,19(5):385-391.
APA Sun, Ya-Xuan,Zheng, Qiu-Sheng,Li, Gang,Guo, De-An,&Wang, Zi-Ren.(2006).Mechanism of ascorbic acid-induced reversion against malignant phenotype in human gastric cancer cells.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,19(5),385-391.
MLA Sun, Ya-Xuan,et al."Mechanism of ascorbic acid-induced reversion against malignant phenotype in human gastric cancer cells".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 19.5(2006):385-391.
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