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Direct effects of lead (Pb2+) on the relaxation of in vitro cultured rat aorta to acetylcholine
Zhang Le-Feng1; Peng Shuang-Qing1; Sheng, Wang1; Li Bian-Lan1; Gang, Han1; Dong Yan-Sheng1
关键词Pb2++ Toxicity Aorta Vascular Reactivity No Enos Inos
刊名TOXICOLOGY LETTERS
2007-04-25
DOI10.1016/j.toxlet.2007.02.004
170期:2页:104-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]SOLUBLE GUANYLATE-CYCLASE ; NITRIC-OXIDE ; INDUCED HYPERTENSION ; SUPEROXIDE-DISMUTASE ; BLOOD-PRESSURE ; VASCULAR WALL ; EXPOSURE ; SYSTEM ; EXPRESSION ; RELEASE
英文摘要

Lead (Pb2+) exposure is related to increased blood pressure or hypertension of human or animals. Abnormal vascular relaxant responses of low level Ph2+ exposed animals were reported by several studies. However, it is difficult to tell whether these effects were induced directly by Pb2+ or not. In this study we hypothesized that Pb2+ can directly affect the relaxation of vessels. Male Wistar rat aortae were removed and cultured in PMRI 1640 with 1 ppm Pb2+ (4.8 mu M lead acetate) for 0.5, 6, 12 and 24 h, and then their responses to acetylcholine (ACh) and sodium nitroprusside (SNP) were examined. After incubated for 24 h, the relaxation induced by ACh was significantly decreased in Pb2+ exposed aortic rings. However, there was not significant difference in relaxation induced by SNP between Pb2+ exposed and control group. The nitrite in the culture media of aortic rings cultured for 24 ht, measured with Griess method, was significantly decreased in the Ph2+ exposed group. The expression of endothelial NOS (eNOS) and isoform NOS (iNOS) in the homogenate of aortic rings cultured for 24 h was measured by Western blot. The expression of eNOS of the Pb2+ exposed group was significantly upregulated compared with that of the control group. However, there was no significant difference in the expression of iNOS in control and Ph2+ exposed group. In conclusion, Pb2+ was able to directly affect the relaxation of rat aorta. This effect may have some relation with the lower level of NO in the media, though the expression of eNOS was upregulated. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000246875200002
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50410
Collection北京大学公共卫生学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Occupat & Environm Med, Beijing 100083, Peoples R China
2.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
3.Capital Med Univ, Sch Publ Hlth & Family Med, Dept Occupat & Environm Med, Beijing 100069, Peoples R China
Recommended Citation
GB/T 7714
Zhang Le-Feng,Peng Shuang-Qing,Sheng, Wang,et al. Direct effects of lead (Pb2+) on the relaxation of in vitro cultured rat aorta to acetylcholine[J]. TOXICOLOGY LETTERS,2007,170(2):104-110.
APA Zhang Le-Feng,Peng Shuang-Qing,Sheng, Wang,Li Bian-Lan,Gang, Han,&Dong Yan-Sheng.(2007).Direct effects of lead (Pb2+) on the relaxation of in vitro cultured rat aorta to acetylcholine.TOXICOLOGY LETTERS,170(2),104-110.
MLA Zhang Le-Feng,et al."Direct effects of lead (Pb2+) on the relaxation of in vitro cultured rat aorta to acetylcholine".TOXICOLOGY LETTERS 170.2(2007):104-110.
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