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Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma
Feng, Jie1,2; Yang, Xiu-Wei1; Huang, Ren-Bin2; Zhang, Hai-Ying2; He, Min2; Huang, Qi-Chun3
关键词Nitidine Chloride Lc-esi-ms/ms Pharmacokinetics
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2012-03-01
DOI10.1016/j.jchromb.2012.01.006
887页:43-47
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者Natural Science Foundation of Guangxi Province of China ; Chinese Medicine Science and Technology Development of Guangxi Administration of TCM ; Doctor foundation of Guangxi Medical University ; Natural Science Foundation of Guangxi Province of China ; Chinese Medicine Science and Technology Development of Guangxi Administration of TCM ; Doctor foundation of Guangxi Medical University
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]IN-VITRO
英文摘要

A new liquid chromatography-electrospray ionization-mass/mass spectrometry (LC-ESI-MS/MS) assay method has been developed and validated for the quantification of nitidine chloride (NC), an anti-cancer bioactive substance of Zanthoxylum nitidum (Roxb.) DC. plants, in rat plasma using carbamazepine as an internal standard (I.S.). The NC and I.S. were extracted from rat plasma by acetonitrile protein procedure. Chromatographic separation was carried out with a C-18 column (2.1 mm x 150 mm, 3 mu m) with a security guard C-18 column (4 mm x 20 mm, 3 mu m). The mobile phase consisted of acetonitrile-10 mM ammonium acetate buffer solution-formic acid (35:65:0.2, v/v/v) and delivered at the flow rate of 0.25 mL/min. LC-ESI-MS/MS was performed on a triple-quadrupole mass spectrometry equipped with electrospray ionization (ESI) and positive multiple reaction monitoring (MRM). Target ions were monitored at [M](+) m/z 348.2 for NC and [M](+) m/z 237.2 for I.S. The method was linear over the concentration range of 5.0-1500.0 ng/mL. The intra- and inter-day relative standard deviations of the assay were less than 5.0%. The lower limit of quantification was 5.0 ng/mL.. The developed method was successfully applied to the estimation of the pharmacokinetic parameters of NC by intravenous administration to rats. (c) 2012 Elsevier By. All rights reserved.

语种英语
所属项目编号2010GXNSFA013187 ; GZKZ1143
资助者Natural Science Foundation of Guangxi Province of China ; Chinese Medicine Science and Technology Development of Guangxi Administration of TCM ; Doctor foundation of Guangxi Medical University ; Natural Science Foundation of Guangxi Province of China ; Chinese Medicine Science and Technology Development of Guangxi Administration of TCM ; Doctor foundation of Guangxi Medical University
WOS记录号WOS:000302049600006
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50419
Collection北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Guangxi Med Univ, Sch Pharmaceut Sci, Nanning 530021, Guangxi, Peoples R China
3.Guangxi Med Univ, Tumor Hosp, Guangxi Tumor Inst & Hosp, Nanning 530021, Guangxi, Peoples R China
Recommended Citation
GB/T 7714
Feng, Jie,Yang, Xiu-Wei,Huang, Ren-Bin,et al. Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,887:43-47.
APA Feng, Jie,Yang, Xiu-Wei,Huang, Ren-Bin,Zhang, Hai-Ying,He, Min,&Huang, Qi-Chun.(2012).Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,887,43-47.
MLA Feng, Jie,et al."Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 887(2012):43-47.
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