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学科主题: 药学
题名:
Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma
作者: Feng, Jie1,2; Yang, Xiu-Wei1; Huang, Ren-Bin2; Zhang, Hai-Ying2; He, Min2; Huang, Qi-Chun3
关键词: Nitidine chloride ; LC-ESI-MS/MS ; Pharmacokinetics
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2012-03-01
DOI: 10.1016/j.jchromb.2012.01.006
卷: 887, 页:43-47
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: IN-VITRO
英文摘要:

A new liquid chromatography-electrospray ionization-mass/mass spectrometry (LC-ESI-MS/MS) assay method has been developed and validated for the quantification of nitidine chloride (NC), an anti-cancer bioactive substance of Zanthoxylum nitidum (Roxb.) DC. plants, in rat plasma using carbamazepine as an internal standard (I.S.). The NC and I.S. were extracted from rat plasma by acetonitrile protein procedure. Chromatographic separation was carried out with a C-18 column (2.1 mm x 150 mm, 3 mu m) with a security guard C-18 column (4 mm x 20 mm, 3 mu m). The mobile phase consisted of acetonitrile-10 mM ammonium acetate buffer solution-formic acid (35:65:0.2, v/v/v) and delivered at the flow rate of 0.25 mL/min. LC-ESI-MS/MS was performed on a triple-quadrupole mass spectrometry equipped with electrospray ionization (ESI) and positive multiple reaction monitoring (MRM). Target ions were monitored at [M](+) m/z 348.2 for NC and [M](+) m/z 237.2 for I.S. The method was linear over the concentration range of 5.0-1500.0 ng/mL. The intra- and inter-day relative standard deviations of the assay were less than 5.0%. The lower limit of quantification was 5.0 ng/mL.. The developed method was successfully applied to the estimation of the pharmacokinetic parameters of NC by intravenous administration to rats. (c) 2012 Elsevier By. All rights reserved.

语种: 英语
所属项目编号: 2010GXNSFA013187 ; GZKZ1143
项目资助者: Natural Science Foundation of Guangxi Province of China ; Chinese Medicine Science and Technology Development of Guangxi Administration of TCM ; Doctor foundation of Guangxi Medical University
WOS记录号: WOS:000302049600006
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50419
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Guangxi Med Univ, Sch Pharmaceut Sci, Nanning 530021, Guangxi, Peoples R China
3.Guangxi Med Univ, Tumor Hosp, Guangxi Tumor Inst & Hosp, Nanning 530021, Guangxi, Peoples R China

Recommended Citation:
Feng, Jie,Yang, Xiu-Wei,Huang, Ren-Bin,et al. Development and validation of an LC-ESI-MS/MS method for the determination of nitidine chloride in rat plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,887:43-47.
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