|The role of muscarinic acetylcholine receptor type 3 polypeptide (M3RP(205-220)) antibody in the saliva of patients with primary Sjogren′s syndrome|
|He, J.1; Qiang, L.2,3; Ding, Y.1; Wei, P.2,3; Li, Y. -N.1; Hua, H.2,3; Li, Z. -G.1|
|关键词||Primary Sjogren&Prime s Syndrome Saliva Type 3 Muscarinic Acetylcholine Receptor|
|刊名||CLINICAL AND EXPERIMENTAL RHEUMATOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||REVISED CRITERIA ; AUTOANTIBODIES ; CLASSIFICATION|
Sjogren′s syndrome (SS) is a chronic autoimmune disorder of unknown cause. Recent studies have shown that anti-muscarinic acetylcholine type 3 receptor (M3R) antibodies can be detected in patients with Sjogren′s syndrome (SS), but little is known about the diagnostic value of this antibody.
To assess the clinical correlations of anti-M3R (muscarinic acetylcholine receptor type 3) polypeptide (M3RP(205-220)) antibodies in saliva from patients of primary Sjogren′s syndrome (pSS).
Serum samples and unstimulated mixed saliva from 100 patients with SS were collected and examined. Their mean (SD) age was 54.2 (13.4) years, and the mean (SD) disease duration was 6.2 (3.8) years. Serum samples from 40 patients with systemic lupus erythematosus (SLE), 40 with rheumatoid arthritis (RA), and 60 healthy subjects were analysed as controls. All the patients with SS were carefully evaluated according to European and American criteria. A circular M3RP(205-220) peptide sequence was synthesized using solid-phase techniques on an applied biosytems peptide synthesizer. The correlation between anti-M3RP(205-220) antibodies and clinical manifestations of pSS was analysed.
The IgG of anti-M3RP(205-220) antibodies was present in 69% of patients with pSS, 27.5% with SLE, 22.5% with RA, and 23.3% of normal saliva donors. The prevalence of anti-M3RP(205-220) antibodies in pSS was significantly higher than in SLE, RA, and normal controls. The specificity of anti-M3RP(205-220) antibodies in pSS was 75%. The salivary flow rate in the group positive for anti-M3RP(205-220) was 436 mu l/10 min, compared to a rate of 658 mu l/10 min for the negative group (p<0.05).
The anti-M3RP(205-220) antibody was detected in most patients with pSS. The presence of the antibody was closely associated with the salivary flow rate. This indicated that it may act as an autoantigen, with a role in the pathogenesis of pSS.
|项目编号||30973318 ; 31070788 ; 7102155|
|资助机构||National Natural Science Foundation of China ; Beijing Natural Science Foundation|
|作者单位||1.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China|
2.Peking Univ, Dept Oral Med, Sch Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Dept Oral Med, Hosp Stomatol, Beijing 100081, Peoples R China
|He, J.,Qiang, L.,Ding, Y.,et al. The role of muscarinic acetylcholine receptor type 3 polypeptide (M3RP(205-220)) antibody in the saliva of patients with primary Sjogren′s syndrome[J]. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY,2012,30(3):322-326.|
|APA||He, J..,Qiang, L..,Ding, Y..,Wei, P..,Li, Y. -N..,...&Li, Z. -G..(2012).The role of muscarinic acetylcholine receptor type 3 polypeptide (M3RP(205-220)) antibody in the saliva of patients with primary Sjogren′s syndrome.CLINICAL AND EXPERIMENTAL RHEUMATOLOGY,30(3),322-326.|
|MLA||He, J.,et al."The role of muscarinic acetylcholine receptor type 3 polypeptide (M3RP(205-220)) antibody in the saliva of patients with primary Sjogren′s syndrome".CLINICAL AND EXPERIMENTAL RHEUMATOLOGY 30.3(2012):322-326.|