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学科主题: 临床医学
题名:
Metabolic profiling analysis of a D-galactosamine/lipopolysaccharide-induced mouse model of fulminant hepatic failure
作者: Feng, Bo1; Wu, Shengming1; Lv, Sa1; Liu, Feng1; Chen, Hongsong1; Yan, Xianzhong1; Li, Yu1; Dong, Fangting1; Wei, Lai1
关键词: metabonomics ; GC/MS ; fulminant hepatic failure
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007
DOI: 10.1021/pr0606326
卷: 6, 期:6, 页:2161-2167
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: ACUTE LIVER-FAILURE ; UNITED-STATES MULTICENTER ; D-GALACTOSAMINE ; AMINO-ACID ; RAT-LIVER ; H-1-NMR-BASED METABONOMICS ; FUNCTIONAL GENOMICS ; SERUM PHOSPHATE ; EARLY PREDICTOR ; BLOOD LACTATE
英文摘要:

The purpose of this study was to characterize the changes in metabolic intermediates and to investigate the metabolic profile of a mouse model of fulminant hepatic failure (FHF), induced by D-galactosamine/ lipopolysaccharide (GaIN/LPS). Plasma metabolite levels were detected using gas chromatography/ time-of-flight mass spectrometry, and the acquired data were transferred into Simca-P and processed using principal components analysis ( PCA). In total, 45 metabolites were identified from the 267 distinct compounds found in the study. Whereas significant differences were noted in the plasma levels of the control and FHF groups, no differences in gluconeogenesis or glycolysis were noted following GaIN/LPS treatment. Our data also suggest that the production of ketone bodies, and the tricarboxylic acid and urea cycles, was inhibited. PCA data suggest that 5-hydroxyindoleacetic acid, glucose, beta-hydroxybutyrate, and phosphate parameters had the highest weights on each of the principal components, and that they were the most important metabolites contributing to the separation of groups. In conclusion, this metabonomic approach can be used as a powerful tool to characterize changes in metabolic intermediates and to search for metabolic markers under certain pathophysiological conditions, such as FHF. Our data also demonstrate that a combination of 5-hydroxyindoleacetic acid, glucose, beta-hydroxybutyrate, and phosphate concentrations in the plasma is a potential marker for FHF, as well as for the early prognosis of FHF.

语种: 英语
WOS记录号: WOS:000246893500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50439
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
2.Natl Ctr Biomed Anal, Beijing 100039, Peoples R China

Recommended Citation:
Feng, Bo,Wu, Shengming,Lv, Sa,et al. Metabolic profiling analysis of a D-galactosamine/lipopolysaccharide-induced mouse model of fulminant hepatic failure[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(6):2161-2167.
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