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学科主题临床医学
Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro
Liu, JJ()1; Huang, RW()1; Lin, DJ()1; Wu, XY()1; Lin, Q()1; Peng, J()1; Pan, XL()1; Song, YQ()1; Zhang, MH()1; Hou, M()1; Chen, F()1
关键词Ponicidin Caspase-3 Parp Bcl-2 Bax
刊名ONCOLOGY REPORTS
2005-04-01
13期:4页:653-657
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]ENT-KAURANE DITERPENOIDS ; CANCER-THERAPY ; APOPTOSIS ; ACTIVATION ; CASPASES ; INDUCTION ; RUBESCENS ; PROTEIN ; BAX
英文摘要

Ponicidin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. Ponicidin has been reported to have anti-tumor effects on a large variety of malignant diseases. In this study, we investigated the antiproliferation effects of ponicidin on human myeloid K562 and HL-60 cells. Cell viability was measured by MTT assay; cell apoptosis was assessed by flow cytometry, DNA fragmentation analysis and Hoechst 33258 staining. Caspase-3 and poly(ADP-ribose) polymerase (PARP) activation and Bax and Bcl-2 expression were detected by Western blot analysis. The results revealed that ponicidin could significantly inhibit the growth of K562 and HL-60 cells by induction of apoptosis. The suppression was both time- and dose-dependent. Cell apoptosis was observed clearly after the cells were treated with ponicidin for 48-72 h. Western blotting showed cleavage of the caspase-3 zymogen protein (32 kDa) with the appearance of its 17 kDa subunit, together with a cleaved 89-kDa fragment of 116 kDa PARP when apoptosis occurred. Bcl-2 expression was down-regulated while Bax expression up-regulated concurrently when the cells were treated with ponicidin for 24-48 h. Therefore, we conclude that ponicidin has significant anti-proliferation effects by induction of apoptosis on myeloid leukemia cells in vitro, down-regulation of Bcl-2, and up-regulation of Bax, and that activation of caspase-3 and PARP may be an important apoptosis-inducing mechanism. The results suggest that ponicidin may serve as a potential therapeutic agent for leukemia.

语种英语
WOS记录号WOS:000227794100013
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50444
专题北京大学第三临床医学院_肿瘤放疗科
作者单位1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Haematol & Oncol, Guangzhou 510630, Guangdong, Peoples R China
2.Shandong Univ, QiLU Hosp, Dept Hematol & Oncol, Shandong 250012, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Oncol, Beijing 100083, Peoples R China
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GB/T 7714
Liu, JJ,Huang, RW,Lin, DJ,et al. Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro[J]. ONCOLOGY REPORTS,2005,13(4):653-657.
APA Liu, JJ.,Huang, RW.,Lin, DJ.,Wu, XY.,Lin, Q.,...&Chen, F.(2005).Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro.ONCOLOGY REPORTS,13(4),653-657.
MLA Liu, JJ,et al."Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro".ONCOLOGY REPORTS 13.4(2005):653-657.
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