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学科主题: 临床医学
题名:
Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro
作者: Liu, JJ1; Huang, RW1; Lin, DJ1; Wu, XY1; Lin, Q1; Peng, J1; Pan, XL1; Song, YQ1; Zhang, MH1; Hou, M1; Chen, F1
关键词: ponicidin ; caspase-3 ; PARP ; Bcl-2 ; Bax
刊名: ONCOLOGY REPORTS
发表日期: 2005-04-01
卷: 13, 期:4, 页:653-657
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: ENT-KAURANE DITERPENOIDS ; CANCER-THERAPY ; APOPTOSIS ; ACTIVATION ; CASPASES ; INDUCTION ; RUBESCENS ; PROTEIN ; BAX
英文摘要:

Ponicidin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. Ponicidin has been reported to have anti-tumor effects on a large variety of malignant diseases. In this study, we investigated the antiproliferation effects of ponicidin on human myeloid K562 and HL-60 cells. Cell viability was measured by MTT assay; cell apoptosis was assessed by flow cytometry, DNA fragmentation analysis and Hoechst 33258 staining. Caspase-3 and poly(ADP-ribose) polymerase (PARP) activation and Bax and Bcl-2 expression were detected by Western blot analysis. The results revealed that ponicidin could significantly inhibit the growth of K562 and HL-60 cells by induction of apoptosis. The suppression was both time- and dose-dependent. Cell apoptosis was observed clearly after the cells were treated with ponicidin for 48-72 h. Western blotting showed cleavage of the caspase-3 zymogen protein (32 kDa) with the appearance of its 17 kDa subunit, together with a cleaved 89-kDa fragment of 116 kDa PARP when apoptosis occurred. Bcl-2 expression was down-regulated while Bax expression up-regulated concurrently when the cells were treated with ponicidin for 24-48 h. Therefore, we conclude that ponicidin has significant anti-proliferation effects by induction of apoptosis on myeloid leukemia cells in vitro, down-regulation of Bcl-2, and up-regulation of Bax, and that activation of caspase-3 and PARP may be an important apoptosis-inducing mechanism. The results suggest that ponicidin may serve as a potential therapeutic agent for leukemia.

语种: 英语
WOS记录号: WOS:000227794100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50444
Appears in Collections:北京大学第三临床医学院_肿瘤放疗科_期刊论文

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作者单位: 1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Haematol & Oncol, Guangzhou 510630, Guangdong, Peoples R China
2.Shandong Univ, QiLU Hosp, Dept Hematol & Oncol, Shandong 250012, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Oncol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, JJ,Huang, RW,Lin, DJ,et al. Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro[J]. ONCOLOGY REPORTS,2005,13(4):653-657.
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