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ATM-mediated NuSAP phosphorylation induces mitotic arrest
Xie, Ping1,2; Li, Lu1; Xing, Guichun1; Tian, Chunyan1; Yin, Yuxin3; He, Fuchu1,2; Zhang, Lingqiang1
关键词NuSAP ATM Spindle assembly Phosphorylation G2/M-phase
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2011-01-07
DOI10.1016/j.bbrc.2010.11.135
404期:1页:413-418
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
资助者Chinese National Basic Research Programs ; National Key Technologies R& ; D Program for New Drugs ; Chinese National Basic Research Programs ; National Key Technologies R& ; D Program for New Drugs
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ATAXIA-TELANGIECTASIA ; CELL-CYCLE ; PROTEIN-KINASES ; DNA-DAMAGE ; SPINDLE ; CHECKPOINT ; P53 ; MICROTUBULES ; CENTROSOME ; ACTIVATION
英文摘要

NuSAP is a microtubule-associated protein that plays an important role in spindle assembly. NuSAP deficiency in mice leads to early embryonic lethality. Spindle assembly in NuSAP-deficient cells is highly inefficient and chromosomes remain dispersed in the mitotic cytoplasm. ATM is a key kinase that phosphorylates a series of substrates to mediate G1/S control. However, the role of ATM at the G2/M phase is not well understood. Here we demonstrate that ectopic expression of NuSAP lead to mitotic arrest observably dependent on the kinase activity of ATM. When endogenous ATM was depleted or its kinase activity was inhibited. NuSAP could not cause mitotic arrest. We further show ATM interacts with NuSAP and phosphorylates NuSAP on Ser124. The phosphorylation and interaction occur specifically at G2/M-phase. Collectively, our work has uncovered an ATM-dependent checkpoint pathway that prevents mitotic progression by targeting a microtubule-associated protein, NuSAP. (C) 2010 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2007CB914601 ; 2010CB912202 ; 2009ZX09503-002 ; 2009ZX09301-002
资助者Chinese National Basic Research Programs ; National Key Technologies R& ; D Program for New Drugs ; Chinese National Basic Research Programs ; National Key Technologies R& ; D Program for New Drugs
WOS记录号WOS:000286487700073
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50456
专题基础医学院_病理学系
作者单位1.Beijing Inst Radiat Med, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing 100850, Peoples R China
2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
3.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xie, Ping,Li, Lu,Xing, Guichun,et al. ATM-mediated NuSAP phosphorylation induces mitotic arrest[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,404(1):413-418.
APA Xie, Ping.,Li, Lu.,Xing, Guichun.,Tian, Chunyan.,Yin, Yuxin.,...&Zhang, Lingqiang.(2011).ATM-mediated NuSAP phosphorylation induces mitotic arrest.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,404(1),413-418.
MLA Xie, Ping,et al."ATM-mediated NuSAP phosphorylation induces mitotic arrest".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 404.1(2011):413-418.
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