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Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis
Yan, Li-Ying1; Qiao, Jie1; Chen, Yuan1; Huang, Jin1; Liu, Ping1; Sun, Qing-Yuan2
关键词Paraformaldehyde Fixation Permeabilized Method 3d-fish Pgd
刊名FERTILITY AND STERILITY
2009-10-01
DOI10.1016/j.fertnstert.2008.08.070
92期:4页:1492-1495
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Major State Basic Research Development Program of the People&prime ; s Republic of China
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]GENOME ORGANIZATION ; NUCLEI ; ARCHITECTURE ; CHROMOSOMES ; CELLS
英文摘要

Objective: To report a simple and efficient fluorescence in situ hybridization (FISH) method for preimplantation genetic diagnosis (PGD).

Design: Technique and method.

Setting: A hospital in vitro fertilization (IVF) laboratory.

Patient(s): Women undergoing IVF or intracytoplasmic sperm injection (ICSI).

Intervention(S): None.

Main Outcome Measure(S): The intensity and clarity of signals, technical difficulty, the percentage of successfully treated blastomeres, and blastomere integrity after FISH.

Result(s): This paraformaldehyde fixation technique simplified the process of fixation of blastomeres for PGD without losing blastomeres during fixation. A total of 35 blastomeres derived from 10 arrested embryos or abnormally fertilized eggs (one pronucleus or three pronuclei) were used for three-dimensional (3D) FISH staining. Signals in all blastomeres were obtained successfully by this method. Approximately 0.1 mu L of DNA probe was enough for the detection of the signals in each blastomere, less than the volume (1 mu L) used in the conventional FISH.

Conclusion(s): The 3D-FISH technique for PGD is easy to learn, less damaging to blastomeres, and loses no blastomeres during fixation. It is efficient, feasible, and economic, which allows more patients to benefit from this technique. (Fertil Steril (R) 2009;92:1492-5. (C) 2009 by American Society for Reproductive Medicine.)

语种英语
所属项目编号2007ZB948102
资助者Major State Basic Research Development Program of the People&prime ; s Republic of China ; Major State Basic Research Development Program of the People&prime ; s Republic of China
WOS记录号WOS:000270616100061
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50475
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Yan, Li-Ying,Qiao, Jie,Chen, Yuan,et al. Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis[J]. FERTILITY AND STERILITY,2009,92(4):1492-1495.
APA Yan, Li-Ying,Qiao, Jie,Chen, Yuan,Huang, Jin,Liu, Ping,&Sun, Qing-Yuan.(2009).Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis.FERTILITY AND STERILITY,92(4),1492-1495.
MLA Yan, Li-Ying,et al."Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis".FERTILITY AND STERILITY 92.4(2009):1492-1495.
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