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学科主题: 临床医学
题名:
Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis
作者: Yan, Li-Ying1; Qiao, Jie1; Chen, Yuan1; Huang, Jin1; Liu, Ping1; Sun, Qing-Yuan2
关键词: Paraformaldehyde fixation ; permeabilized method ; 3D-FISH ; PGD
刊名: FERTILITY AND STERILITY
发表日期: 2009-10-01
DOI: 10.1016/j.fertnstert.2008.08.070
卷: 92, 期:4, 页:1492-1495
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: GENOME ORGANIZATION ; NUCLEI ; ARCHITECTURE ; CHROMOSOMES ; CELLS
英文摘要:

Objective: To report a simple and efficient fluorescence in situ hybridization (FISH) method for preimplantation genetic diagnosis (PGD).

Design: Technique and method.

Setting: A hospital in vitro fertilization (IVF) laboratory.

Patient(s): Women undergoing IVF or intracytoplasmic sperm injection (ICSI).

Intervention(S): None.

Main Outcome Measure(S): The intensity and clarity of signals, technical difficulty, the percentage of successfully treated blastomeres, and blastomere integrity after FISH.

Result(s): This paraformaldehyde fixation technique simplified the process of fixation of blastomeres for PGD without losing blastomeres during fixation. A total of 35 blastomeres derived from 10 arrested embryos or abnormally fertilized eggs (one pronucleus or three pronuclei) were used for three-dimensional (3D) FISH staining. Signals in all blastomeres were obtained successfully by this method. Approximately 0.1 mu L of DNA probe was enough for the detection of the signals in each blastomere, less than the volume (1 mu L) used in the conventional FISH.

Conclusion(s): The 3D-FISH technique for PGD is easy to learn, less damaging to blastomeres, and loses no blastomeres during fixation. It is efficient, feasible, and economic, which allows more patients to benefit from this technique. (Fertil Steril (R) 2009;92:1492-5. (C) 2009 by American Society for Reproductive Medicine.)

语种: 英语
所属项目编号: 2007ZB948102
项目资助者: Major State Basic Research Development Program of the People&prime ; s Republic of China
WOS记录号: WOS:000270616100061
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50475
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China

Recommended Citation:
Yan, Li-Ying,Qiao, Jie,Chen, Yuan,et al. Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis[J]. FERTILITY AND STERILITY,2009,92(4):1492-1495.
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