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学科主题: 临床医学
题名:
Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro
作者: Pan, Xiao-Ben; Ma, Hui; Jin, Qian; Wei, Lai
关键词: hepatitis B virus ; HepG2 ; interferon ; microRNA ; peripheral blood mononuclear cell
刊名: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
发表日期: 2012-04-01
DOI: 10.1111/j.1440-1746.2011.06979.x
卷: 27, 期:4, 页:805-812
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: EPSTEIN-BARR-VIRUS ; X PROTEIN ; INTERFERON-ALPHA ; HEPATOCELLULAR-CARCINOMA ; VIRAL MICRORNAS ; CELLS ; INHIBITION ; INFECTION ; THERAPY ; TRANSCRIPTION
英文摘要:

Background and Aim: Alpha interferon (IFN-alpha) is an approved treatment for chronic hepatitis B (CHB). MicroRNA (miRNA) are currently known as a part of IFN-mediated antiviral defense. We aimed at characterizing the miRNA expression associated with hepatitis B virus (HBV) replication and IFN-mediated HBV clearance.

Methods: We investigated the expression patterns of cellular miRNA induced by HBV replication and/or IFN-alpha treatment in HepG2 cells, and also analyzed the miRNA response in peripheral blood mononuclear cells in CHB patients on IFN-a treatment. The differentially expressed miRNA were verified using quantitative real-time polymerase chain reaction and an miRNA expression pattern was classified based on the final virological response.

Results: A total of 223 miRNA were differentially expressed (> 1.5 folds) between the HepG2.2.15 and HepG2 cells, including 24 highly differentially expressed miRNA (> 5 folds). With 12 h of IFN-alpha, treatment, 23 totally differentially expressed miRNA were identified in HepG2 cells; whereas only five miRNA were identified in HepG2.2.15 cells. Similar amounts of the miRNA were regulated in patients with HBeAg or non-HBeAg seroconversion; whereas levels of eight miRNA were significantly differentially expressed between the two groups.

Conclusions: HBV replication alters miRNA expression profiles and impairs IFN-inducible miRNA response in HepG2 cells. The miRNA expression pattern of peripheral blood mononuclear cells in CHB patients with ITN therapy can be associated with their therapeutic outcome.

语种: 英语
所属项目编号: 2008ZX10002-012 ; 2008ZX10002-013 ; 30972605 ; RDC-2009-13
项目资助者: Major National S&amp ; T Projects for Infectious Diseases (11th Five-Year Project) ; National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
WOS记录号: WOS:000301924800028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50481
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Peking Univ Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Pan, Xiao-Ben,Ma, Hui,Jin, Qian,et al. Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2012,27(4):805-812.
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