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学科主题: 临床医学
题名:
Inducing Cell Proliferation Inhibition, Apoptosis, and Motility Reduction by Silencing Long Noncoding Ribonucleic Acid Metastasis-associated Lung Adenocarcinoma Transcript 1 in Urothelial Carcinoma of the Bladder
作者: Han, Yonghua1; Liu, Yuchen1; Nie, Liping1; Gui, Yaoting1; Cai, Zhiming1
刊名: UROLOGY
发表日期: 2013
DOI: 10.1016/j.urology.2012.08.044
卷: 81, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: GENE-EXPRESSION ; CANCER ; RNAS ; INVASION ; MALAT-1
英文摘要:

OBJECTIVE To study the expression patterns of long noncoding ribonucleic acid (RNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and the cell proliferation inhibition, apoptosis, and motility changes induced by silencing MALAT1 in urothelial carcinoma of the bladder.

MATERIALS AND METHODS The expression levels of MALAT1 were determined using real-time quantitative polymerase chain reaction in cancerous tissues and paired normal tissues in a total of 36 patients with urothelial carcinoma of the bladder. Expression differences were analyzed according to the grade and stage. Bladder urothelial carcinoma T24 and 5637 cells were transfected with MALAT1 small interfering RNA or negative control small interfering RNA. The cell proliferation changes of the transfected bladder urothelial carcinoma cells were determined using the MTT assay. Apoptosis caused by silencing MALAT1 was evaluated using the flow cytometry assay and enzyme-linked immunosorbent assay. The motility changes induced by silencing MALAT1 were measured using the wound healing assay.

RESULTS MALAT1 was upregulated in bladder urothelial carcinoma compared with matched normal urothelium (P = .008). The MALAT1 expression levels were greater in high-grade carcinomas than in low-grade carcinoma (P = .001). The MALAT1 expression levels were greater in invasive carcinoma than in noninvasive carcinoma (P = .018). Cell proliferation inhibition, increased apoptosis, and decreased motility were observed in MALAT1 small interfering RNA-transfected bladder urothelial carcinoma T24 and 5637 cells.

CONCLUSION MALAT1 plays an oncogenic role in urothelial carcinoma of the bladder. Silencing MALAT1 is a potential novel therapeutic approach for this cancer. UROLOGY 81:209.e1-209.e7, 2013. (C) 2013 Elsevier Inc.

语种: 英语
所属项目编号: 2009AA022707 ; CXB200903090055A ; CXB201005250016A ; CXC201005260001A
项目资助者: National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen
WOS记录号: WOS:000312960300064
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50487
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shenzhen PKU HKUST Med Ctr, Inst Urol, Shenzhen, Peoples R China
2.Peking Univ, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen Hosp, Shenzhen 518036, Peoples R China
3.Shenzhen Second Peoples Hosp, Cent Lab, Shenzhen, Peoples R China
4.Anhui Med Univ, Hefei, Peoples R China
5.Peking Univ, Dept Clin Lab, Shenzhen Hosp, Shenzhen 518036, Peoples R China

Recommended Citation:
Han, Yonghua,Liu, Yuchen,Nie, Liping,et al. Inducing Cell Proliferation Inhibition, Apoptosis, and Motility Reduction by Silencing Long Noncoding Ribonucleic Acid Metastasis-associated Lung Adenocarcinoma Transcript 1 in Urothelial Carcinoma of the Bladder[J]. UROLOGY,2013,81(1).
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