学科主题 | 临床医学 |
Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis | |
Chu, Liqun; Li, Xiaoxin; Yu, Wenzen; Qian, Tong; Qi, Huijun; Huang, Luzhen; Xu, Yongsheng | |
关键词 | Endotoxin-induced Uveitis Fractalkine Chemokine Chemoattractant |
刊名 | OPHTHALMIC RESEARCH
![]() |
2009 | |
DOI | 10.1159/000230878 |
卷 | 42期:3页:160-166 |
收录类别 | SCI |
文章类型 | Article |
WOS标题词 | Science & Technology |
类目[WOS] | Ophthalmology |
资助者 | China Natural Science Foundation ; China Postdoctoral Science Foundation ; China Natural Science Foundation ; China Postdoctoral Science Foundation |
研究领域[WOS] | Ophthalmology |
关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; RETINAL MICROGLIA ; RAT MODEL ; CHEMOKINES ; ACTIVATION ; DISEASE ; CELLS ; PAIN ; CNS ; DEGENERATION |
英文摘要 | Background/Aims: Chemokines play a critical role in inflammation and neurodegenerative disease in the central nervous system. In this study, endotoxin-induced uveitis (EIU) was induced to test the expression of fractalkine, a special neuronal chemokine, and its receptor CX3CR1 in acute inflammation of the retina. Methods: EIU was induced by footpad injections of lipopolysaccharide (LPS). Eight rats were sacrificed at each time point (0, 8, 16, 24, 48, and 72 h) after LPS injection. Sections were made for histopathological tests. Immunohistochemistry was performed using antibodies specific to fractalkine and CX3CR1. Retinas were collected, and total protein and mRNA from both the induced and control rats were extracted. mRNA and protein expression of fractalkine and CX3CR1 in the retina were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blots, respectively. Results: The EIU model was successfully induced. In control rats, both fractalkine and its receptor CX3CR1 were detected in the retina. LPS injection induced a transient upregulation of both proteins at 24 h as determined by the increased number of positively stained cells as well as increased levels of mRNA and protein (p < 0.05). Conclusion: A transitory increased expression of fractalkine and its receptor CX3CR1 occurred at the crest time of EIU, and this change in expression may play a role in the turnover of LPS-induced acute retina inflammation. Copyright (C) 2009 S. Karger AG, Basel |
语种 | 英语 |
所属项目编号 | 30700919 ; 20070410028 |
资助者 | China Natural Science Foundation ; China Postdoctoral Science Foundation ; China Natural Science Foundation ; China Postdoctoral Science Foundation |
WOS记录号 | WOS:000271535500009 |
Citation statistics | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.bjmu.edu.cn/handle/400002259/50489 |
Collection | 北京大学第二临床医学院_眼科 |
作者单位 | Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100871, Peoples R China |
Recommended Citation GB/T 7714 | Chu, Liqun,Li, Xiaoxin,Yu, Wenzen,et al. Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis[J]. OPHTHALMIC RESEARCH,2009,42(3):160-166. |
APA | Chu, Liqun.,Li, Xiaoxin.,Yu, Wenzen.,Qian, Tong.,Qi, Huijun.,...&Xu, Yongsheng.(2009).Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis.OPHTHALMIC RESEARCH,42(3),160-166. |
MLA | Chu, Liqun,et al."Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis".OPHTHALMIC RESEARCH 42.3(2009):160-166. |
Files in This Item: | There are no files associated with this item. |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment