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学科主题临床医学
Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis
Chu, Liqun; Li, Xiaoxin; Yu, Wenzen; Qian, Tong; Qi, Huijun; Huang, Luzhen; Xu, Yongsheng
关键词Endotoxin-induced Uveitis Fractalkine Chemokine Chemoattractant
刊名OPHTHALMIC RESEARCH
2009
DOI10.1159/000230878
42期:3页:160-166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]CENTRAL-NERVOUS-SYSTEM ; RETINAL MICROGLIA ; RAT MODEL ; CHEMOKINES ; ACTIVATION ; DISEASE ; CELLS ; PAIN ; CNS ; DEGENERATION
英文摘要

Background/Aims: Chemokines play a critical role in inflammation and neurodegenerative disease in the central nervous system. In this study, endotoxin-induced uveitis (EIU) was induced to test the expression of fractalkine, a special neuronal chemokine, and its receptor CX3CR1 in acute inflammation of the retina. Methods: EIU was induced by footpad injections of lipopolysaccharide (LPS). Eight rats were sacrificed at each time point (0, 8, 16, 24, 48, and 72 h) after LPS injection. Sections were made for histopathological tests. Immunohistochemistry was performed using antibodies specific to fractalkine and CX3CR1. Retinas were collected, and total protein and mRNA from both the induced and control rats were extracted. mRNA and protein expression of fractalkine and CX3CR1 in the retina were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blots, respectively. Results: The EIU model was successfully induced. In control rats, both fractalkine and its receptor CX3CR1 were detected in the retina. LPS injection induced a transient upregulation of both proteins at 24 h as determined by the increased number of positively stained cells as well as increased levels of mRNA and protein (p < 0.05). Conclusion: A transitory increased expression of fractalkine and its receptor CX3CR1 occurred at the crest time of EIU, and this change in expression may play a role in the turnover of LPS-induced acute retina inflammation. Copyright (C) 2009 S. Karger AG, Basel

语种英语
WOS记录号WOS:000271535500009
项目编号30700919 ; 20070410028
资助机构China Natural Science Foundation ; China Postdoctoral Science Foundation
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50489
专题北京大学第二临床医学院_眼科
作者单位Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100871, Peoples R China
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GB/T 7714
Chu, Liqun,Li, Xiaoxin,Yu, Wenzen,et al. Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis[J]. OPHTHALMIC RESEARCH,2009,42(3):160-166.
APA Chu, Liqun.,Li, Xiaoxin.,Yu, Wenzen.,Qian, Tong.,Qi, Huijun.,...&Xu, Yongsheng.(2009).Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis.OPHTHALMIC RESEARCH,42(3),160-166.
MLA Chu, Liqun,et al."Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis".OPHTHALMIC RESEARCH 42.3(2009):160-166.
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