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学科主题: 临床医学
题名:
Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis
作者: Chu, Liqun; Li, Xiaoxin; Yu, Wenzen; Qian, Tong; Qi, Huijun; Huang, Luzhen; Xu, Yongsheng
关键词: Endotoxin-induced uveitis ; Fractalkine ; Chemokine ; Chemoattractant
刊名: OPHTHALMIC RESEARCH
发表日期: 2009
DOI: 10.1159/000230878
卷: 42, 期:3, 页:160-166
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM ; RETINAL MICROGLIA ; RAT MODEL ; CHEMOKINES ; ACTIVATION ; DISEASE ; CELLS ; PAIN ; CNS ; DEGENERATION
英文摘要:

Background/Aims: Chemokines play a critical role in inflammation and neurodegenerative disease in the central nervous system. In this study, endotoxin-induced uveitis (EIU) was induced to test the expression of fractalkine, a special neuronal chemokine, and its receptor CX3CR1 in acute inflammation of the retina. Methods: EIU was induced by footpad injections of lipopolysaccharide (LPS). Eight rats were sacrificed at each time point (0, 8, 16, 24, 48, and 72 h) after LPS injection. Sections were made for histopathological tests. Immunohistochemistry was performed using antibodies specific to fractalkine and CX3CR1. Retinas were collected, and total protein and mRNA from both the induced and control rats were extracted. mRNA and protein expression of fractalkine and CX3CR1 in the retina were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blots, respectively. Results: The EIU model was successfully induced. In control rats, both fractalkine and its receptor CX3CR1 were detected in the retina. LPS injection induced a transient upregulation of both proteins at 24 h as determined by the increased number of positively stained cells as well as increased levels of mRNA and protein (p < 0.05). Conclusion: A transitory increased expression of fractalkine and its receptor CX3CR1 occurred at the crest time of EIU, and this change in expression may play a role in the turnover of LPS-induced acute retina inflammation. Copyright (C) 2009 S. Karger AG, Basel

语种: 英语
所属项目编号: 30700919 ; 20070410028
项目资助者: China Natural Science Foundation ; China Postdoctoral Science Foundation
WOS记录号: WOS:000271535500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50489
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Chu, Liqun,Li, Xiaoxin,Yu, Wenzen,et al. Expression of Fractalkine (CX3CL1) and Its Receptor in Endotoxin-Induced Uveitis[J]. OPHTHALMIC RESEARCH,2009,42(3):160-166.
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