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学科主题: 临床医学
题名:
Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes
作者: Lin, Lin1; Shen, Qi2; Zhang, Chenguang3; Chen, Lianxu1; Yu, Changlong1
关键词: chondrocyte ; dedifferentiation ; microRNA ; microarray analysis
刊名: JOURNAL OF ORTHOPAEDIC RESEARCH
发表日期: 2011-10-01
DOI: 10.1002/jor.21423
卷: 29, 期:10, 页:1578-1584
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Orthopedics
研究领域[WOS]: Orthopedics
关键词[WOS]: CARTILAGE DEFECTS ; GENE-EXPRESSION ; PHENOTYPE ; TRANSPLANTATION ; CULTURE ; TISSUE ; KNEE
英文摘要:

MicroRNA has an important role in regulating gene expression during cell differentiation. In this study we identified the expression pattern of microRNA in the differentiated and dedifferentiated chondrocytes. Adult human articular chondrocytes were cultured in monolayer. RNA was isolated from the differentiated chondrocytes (collected after isolation) and the fifth-passage (dedifferentiated) chondrocytes, and subjected to gene expression analysis using microRNA and cDNA microarray analysis. Real-time RT-PCR was also performed to confirm the differentially expressed genes. Furthermore, we integrated microRNA and cDNA microarray data together with computational approaches, such as microRNA gene target prediction algorithms, to reveal the role of microRNAs involved in chondrocyte homeostasis. The results showed a dramatic change in expression of microRNA between the two cell types. Thirteen up-regulated and 12 down-regulated microRNAs were detected in differentiated chondroctes. We also revealed microRNA gene target pairs potentially involved in dedifferentiation process. Our results revealed novel findings of differential expression of microRNA in dedifferentiation, and microRNA could have an important role in the maintenance of chondrocytes homeostasis. MicroRNA may be a target for cartilage tissue engineering and regenerative medicine. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:1578-1584, 2011

语种: 英语
所属项目编号: 30900711 ; 2008B04 ; 200802009
项目资助者: National Natural Scientific Foundation of China ; Beijing NOVA Program ; Public Foundation of Ministry of Health
WOS记录号: WOS:000294592800018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50490
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 1, Inst Urol, Beijing 100034, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Lin, Lin,Shen, Qi,Zhang, Chenguang,et al. Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes[J]. JOURNAL OF ORTHOPAEDIC RESEARCH,2011,29(10):1578-1584.
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