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学科主题临床医学
Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes
Lin, Lin1; Shen, Qi2; Zhang, Chenguang3; Chen, Lianxu1; Yu, Changlong1
关键词Chondrocyte Dedifferentiation Microrna Microarray Analysis
刊名JOURNAL OF ORTHOPAEDIC RESEARCH
2011-10-01
DOI10.1002/jor.21423
29期:10页:1578-1584
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics
研究领域[WOS]Orthopedics
关键词[WOS]CARTILAGE DEFECTS ; GENE-EXPRESSION ; PHENOTYPE ; TRANSPLANTATION ; CULTURE ; TISSUE ; KNEE
英文摘要

MicroRNA has an important role in regulating gene expression during cell differentiation. In this study we identified the expression pattern of microRNA in the differentiated and dedifferentiated chondrocytes. Adult human articular chondrocytes were cultured in monolayer. RNA was isolated from the differentiated chondrocytes (collected after isolation) and the fifth-passage (dedifferentiated) chondrocytes, and subjected to gene expression analysis using microRNA and cDNA microarray analysis. Real-time RT-PCR was also performed to confirm the differentially expressed genes. Furthermore, we integrated microRNA and cDNA microarray data together with computational approaches, such as microRNA gene target prediction algorithms, to reveal the role of microRNAs involved in chondrocyte homeostasis. The results showed a dramatic change in expression of microRNA between the two cell types. Thirteen up-regulated and 12 down-regulated microRNAs were detected in differentiated chondroctes. We also revealed microRNA gene target pairs potentially involved in dedifferentiation process. Our results revealed novel findings of differential expression of microRNA in dedifferentiation, and microRNA could have an important role in the maintenance of chondrocytes homeostasis. MicroRNA may be a target for cartilage tissue engineering and regenerative medicine. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:1578-1584, 2011

语种英语
WOS记录号WOS:000294592800018
项目编号30900711 ; 2008B04 ; 200802009
资助机构National Natural Scientific Foundation of China ; Beijing NOVA Program ; Public Foundation of Ministry of Health
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50490
专题北京大学第三临床医学院_运动医学研究所
北京大学精神卫生研究所_护理部
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 1, Inst Urol, Beijing 100034, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Lin, Lin,Shen, Qi,Zhang, Chenguang,et al. Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes[J]. JOURNAL OF ORTHOPAEDIC RESEARCH,2011,29(10):1578-1584.
APA Lin, Lin,Shen, Qi,Zhang, Chenguang,Chen, Lianxu,&Yu, Changlong.(2011).Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes.JOURNAL OF ORTHOPAEDIC RESEARCH,29(10),1578-1584.
MLA Lin, Lin,et al."Assessment of the Profiling MicroRNA Expression of Differentiated and Dedifferentiated Human Adult Articular Chondrocytes".JOURNAL OF ORTHOPAEDIC RESEARCH 29.10(2011):1578-1584.
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