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学科主题: 基础医学
题名:
Screening for novel human genes associated with CRE pathway activation with cell microarray
作者: Tian, Linjie1; Wang, Pingzhang1; Guo, Jinhai1; Wang, Xinyu1; Deng, Weiwei1; Zhang, Chenying1; Fu, Dongxu1; Gao, Xia1; Shi, Taiping1; Ma, Dalong1
关键词: reverse transfection ; cell microarray ; high-throughput screening ; cAMP response element ; dual luciferase reporter system
刊名: GENOMICS
发表日期: 2007-07-01
DOI: 10.1016/j.ygeno.2007.02.004
卷: 90, 期:1, 页:28-34
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]: PROTEIN FUNCTION ; CBP ; COACTIVATOR ; TRANSCRIPTION ; CALCIUM ; DEGRADATION ; EXPRESSION ; APOPTOSIS ; ARRAYS ; DOMAIN
英文摘要:

In this study, cell microarray technology is used to identify novel human genes associated with CRE pathway activation. By reverse transfection, expression plasmids containing full-length cDNAs were cotransfected with the reporter plasmid pCRE-d2EGFP to monitor the activation of the CRE pathway via enhanced green fluorescence protein (EGFP) expression. Of the 575 predominantly novel genes screened, 22 exhibited relatively higher EGFP fluorescence compared with a negative control. After a functional validation with a dual luciferase reporter system that included both cis- and trans-luciferase assays, 4 of the 22 genes (RNF41, C8orf32, C6orf208, and MEIS3P1) were confirmed as CRE-pathway activators. Western blot analysis revealed that RNF41 can promote CREB phosphorylation. These results demonstrate the successful combination of cell microarray technology with this reporting system and the potential of this tool to characterize functions of novel genes in a highly parallel format. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000247826400004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50501
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Human Dis Genom Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Tian, Linjie,Wang, Pingzhang,Guo, Jinhai,et al. Screening for novel human genes associated with CRE pathway activation with cell microarray[J]. GENOMICS,2007,90(1):28-34.
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