IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Screening for novel human genes associated with CRE pathway activation with cell microarray
Tian, Linjie1; Wang, Pingzhang1; Guo, Jinhai1; Wang, Xinyu1; Deng, Weiwei1; Zhang, Chenying1; Fu, Dongxu1; Gao, Xia1; Shi, Taiping1; Ma, Dalong1
关键词reverse transfection cell microarray high-throughput screening cAMP response element dual luciferase reporter system
刊名GENOMICS
2007-07-01
DOI10.1016/j.ygeno.2007.02.004
90期:1页:28-34
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]PROTEIN FUNCTION ; CBP ; COACTIVATOR ; TRANSCRIPTION ; CALCIUM ; DEGRADATION ; EXPRESSION ; APOPTOSIS ; ARRAYS ; DOMAIN
英文摘要

In this study, cell microarray technology is used to identify novel human genes associated with CRE pathway activation. By reverse transfection, expression plasmids containing full-length cDNAs were cotransfected with the reporter plasmid pCRE-d2EGFP to monitor the activation of the CRE pathway via enhanced green fluorescence protein (EGFP) expression. Of the 575 predominantly novel genes screened, 22 exhibited relatively higher EGFP fluorescence compared with a negative control. After a functional validation with a dual luciferase reporter system that included both cis- and trans-luciferase assays, 4 of the 22 genes (RNF41, C8orf32, C6orf208, and MEIS3P1) were confirmed as CRE-pathway activators. Western blot analysis revealed that RNF41 can promote CREB phosphorylation. These results demonstrate the successful combination of cell microarray technology with this reporting system and the potential of this tool to characterize functions of novel genes in a highly parallel format. (c) 2007 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000247826400004
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50501
专题北京大学基础医学院_免疫学系
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Human Dis Genom Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Tian, Linjie,Wang, Pingzhang,Guo, Jinhai,et al. Screening for novel human genes associated with CRE pathway activation with cell microarray[J]. GENOMICS,2007,90(1):28-34.
APA Tian, Linjie.,Wang, Pingzhang.,Guo, Jinhai.,Wang, Xinyu.,Deng, Weiwei.,...&Ma, Dalong.(2007).Screening for novel human genes associated with CRE pathway activation with cell microarray.GENOMICS,90(1),28-34.
MLA Tian, Linjie,et al."Screening for novel human genes associated with CRE pathway activation with cell microarray".GENOMICS 90.1(2007):28-34.
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