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Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay
Zhao, Zuotao1; Kong, Fanrong1; Martinez, Gabriela1; Zeng, Xianyu1; Gottschalk, Marcelo1; Gilbert, Gwendolyn L.1
关键词Streptococcus Agalactiae Bovine Conventional Serotyping Molecular Serotyping
刊名FEMS MICROBIOLOGY LETTERS
2006-10-01
DOI10.1111/j.1574-6968.2006.00428.x
263期:2页:236-239
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]GROUP-B STREPTOCOCCI ; GENOTYPING SYSTEM ; CLASSIFICATION ; MILK
英文摘要

We used a multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay and sequencing of a variable region of the cps cluster to identify serotypes of 140 Streptococcus agalactiae (group B Streptococcus; GBS) isolates from cattle. Only 71 (51%) isolates were typeable using antisera, but molecular serotypes (MS) were assigned to 133 (95%) and 139 (99%) isolates by partial cpsE-cpsF-cpsG sequencing and mPCR/RLB, respectively. Ninety-four isolates (67%) belonged to MS III and most belonged to a molecular serosubtype (msst) III-3, which is uncommon among GBS isolates from humans. Our results demonstrate that cps clusters of bovine GBS differ significantly from those of GBS isolates from humans.

语种英语
WOS记录号WOS:000241383100017
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50506
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Westmead Hosp, ICPMR, CIDM, Westmead, NSW 2145, Australia
2.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China
3.Univ Montreal, Fac Vet Med, Grp Rech Malad Infect Porc, St Hyacinthe, PQ J2S 7C6, Canada
4.Wuhan First Hosp, Dept Dermatol, Wuhan, Hubei, Peoples R China
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GB/T 7714
Zhao, Zuotao,Kong, Fanrong,Martinez, Gabriela,et al. Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay[J]. FEMS MICROBIOLOGY LETTERS,2006,263(2):236-239.
APA Zhao, Zuotao,Kong, Fanrong,Martinez, Gabriela,Zeng, Xianyu,Gottschalk, Marcelo,&Gilbert, Gwendolyn L..(2006).Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay.FEMS MICROBIOLOGY LETTERS,263(2),236-239.
MLA Zhao, Zuotao,et al."Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay".FEMS MICROBIOLOGY LETTERS 263.2(2006):236-239.
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