北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages
作者: Zhou, SX1; Liu, YQ1; Bo, H1; Bian, XC1; Xia, XJ1; Lin, CS1; Wong, VWS1; Lu, ZH1
关键词: expression profilings ; dendritic cells ; macrophages ; one-way ANOVA ; class prediction
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2006-05-26
DOI: 10.1016/j.bbrc.2006.03.125
卷: 344, 期:1, 页:438-445
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: DIFFERENTIATION ; PATTERNS ; SUBSETS ; NORMALIZATION ; PLASTICITY ; REPOSITORY ; PATHWAYS ; INNATE
英文摘要:

Dendritic cells (DCs) and macrophages share some stages in the development and function of antigen presentation. But it is difficult to separate them from their precursors. We used one-way ANOVA (analysis of variances) on murine expression profilings of several hematopoietic cells associated with DCs and macrophages to find the genes with great differences across the cell groups. These groups were the DCs from spleen, cultivated DCs, DC precursors. DC progenitors, DC progenitor cell lines, hematopoietic stern cell (HSC), and bone marrow-derived macrophages. The data of expression profilings were all downloaded from GEO and ArrayExpress database. After the normalization of I I housekeeping genes across 42 arrays, we got 39 genes (44 probesets) by analysis of one-way ANOVA (Bonferroni step-down) with p values cutoff of 0.05. These genes (probesets) could separate the hematopoietic cells well by the methods of unsupervised hierarchical clustering and principal component analysis (PCA). The class prediction also indicated that these genes could separate the precursors of DC and macrophages with 20 arrays composed of 5 cell types with the same normalization. The accuracy rate of class prediction was 90% (18/20). The genes selected by one-way ANOVA included those of MHC (major histocompatibility complex) and defense of immunity, cell adhesion, chemokine or its receptors, and transcription factors. The results indicated that these 39 genes could separate precursors of DC and macrophages very clearly. It was suggested that these genes might represent some important molecules that related with the precursors of DCs and macrophages, and were worthy for further study. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000237408000062
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50513
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S0006291X06005432-main.pdf(691KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Southeast Univ, Chein Shiung Wu Lab, Dept Biol Sci & Med Engn, Nanjing 210096, Peoples R China
2.Chinese Acad Med Sci, Dept Pathol, Inst Basic Med Sci, Sch Basic Med,Peking Union Med Coll, Beijing 100005, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China
5.Chinese Univ Hong Kong, Dept Med & Therapeut, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Zhou, SX,Liu, YQ,Bo, H,et al. Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,344(1):438-445.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, SX]'s Articles
[Liu, YQ]'s Articles
[Bo, H]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, SX]‘s Articles
[Liu, YQ]‘s Articles
[Bo, H]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace