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学科主题基础医学
Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages
Zhou, SX1; Liu, YQ1; Bo, H1; Bian, XC1; Xia, XJ1; Lin, CS1; Wong, VWS1; Lu, ZH1
关键词expression profilings dendritic cells macrophages one-way ANOVA class prediction
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2006-05-26
DOI10.1016/j.bbrc.2006.03.125
344期:1页:438-445
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]DIFFERENTIATION ; PATTERNS ; SUBSETS ; NORMALIZATION ; PLASTICITY ; REPOSITORY ; PATHWAYS ; INNATE
英文摘要

Dendritic cells (DCs) and macrophages share some stages in the development and function of antigen presentation. But it is difficult to separate them from their precursors. We used one-way ANOVA (analysis of variances) on murine expression profilings of several hematopoietic cells associated with DCs and macrophages to find the genes with great differences across the cell groups. These groups were the DCs from spleen, cultivated DCs, DC precursors. DC progenitors, DC progenitor cell lines, hematopoietic stern cell (HSC), and bone marrow-derived macrophages. The data of expression profilings were all downloaded from GEO and ArrayExpress database. After the normalization of I I housekeeping genes across 42 arrays, we got 39 genes (44 probesets) by analysis of one-way ANOVA (Bonferroni step-down) with p values cutoff of 0.05. These genes (probesets) could separate the hematopoietic cells well by the methods of unsupervised hierarchical clustering and principal component analysis (PCA). The class prediction also indicated that these genes could separate the precursors of DC and macrophages with 20 arrays composed of 5 cell types with the same normalization. The accuracy rate of class prediction was 90% (18/20). The genes selected by one-way ANOVA included those of MHC (major histocompatibility complex) and defense of immunity, cell adhesion, chemokine or its receptors, and transcription factors. The results indicated that these 39 genes could separate precursors of DC and macrophages very clearly. It was suggested that these genes might represent some important molecules that related with the precursors of DCs and macrophages, and were worthy for further study. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000237408000062
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文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/50513
专题北京大学基础医学院_免疫学系
作者单位1.Southeast Univ, Chein Shiung Wu Lab, Dept Biol Sci & Med Engn, Nanjing 210096, Peoples R China
2.Chinese Acad Med Sci, Dept Pathol, Inst Basic Med Sci, Sch Basic Med,Peking Union Med Coll, Beijing 100005, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China
5.Chinese Univ Hong Kong, Dept Med & Therapeut, Hong Kong, Hong Kong, Peoples R China
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Zhou, SX,Liu, YQ,Bo, H,et al. Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,344(1):438-445.
APA Zhou, SX.,Liu, YQ.,Bo, H.,Bian, XC.,Xia, XJ.,...&Lu, ZH.(2006).Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,344(1),438-445.
MLA Zhou, SX,et al."Expression profilings of 39 genes selected by ANOVA could separate precursors of murine dendritic cells and macrophages".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 344.1(2006):438-445.
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