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学科主题: 药学
题名:
Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography
作者: Liu, RX1; Wang, Q1; Guo, HZ1; Li, L1; Bi, KS1; Guo, DA1
关键词: Dalbergia odorifera ; flavonoids ; reversed-phase high performance liquid chromatography
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2005-09-15
DOI: 10.1016/j.jpba.2005.04.007
卷: 39, 期:3-4, 页:469-476
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: RED-CLOVER ; BUTEIN ; CONSTITUENTS ; ISOFLAVONES ; INHIBITOR ; HEARTWOOD ; APOPTOSIS ; CELLS ; HPLC
英文摘要:

A reversed-phase liquid chromatographic method was developed for the simultaneous quantification of 10 major flavonoids, namely butin, (3R)-4′-methoxy-2′,3,7-trihydroxyisoflavanone, liquiritigenin, melanettin, violanone, vistitone, formononetin, dalbergin, sativanone and medicarpin in the heartwood of Dalbergia odorifera, an important traditional Chinese medicine. Samples were extracted with 60% methanol. The optimal conditions of separation and detection were achieved on an AgilentZorbax SB-C-18 column (250mm x 4.6mm, 5 mu m) with a gradient of acetonitrile and 0.3% (v/v) aqueous acetic acid, at a flow rate of 0.8 ml/min, detected at 275 nm. The complete separation was obtained within 55 min for the 10 target compounds. All calibration curves showed good linearity (r(2) > 0.999) within test ranges. The assay was reproducible with overall intra- and inter-day variation of less than 3%. The mean recovery of the method was 100 +/- 1 0%, with R.S.D. less than 5%. The current assay method was considered to be suitable for the quality control of D. odorifera samples and could be readily utilized for the determination of the active principles present in this medicinal herb. (c) 2005 Elsevier B.V. All fights reserved.

语种: 英语
WOS记录号: WOS:000232209700014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50518
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China

Recommended Citation:
Liu, RX,Wang, Q,Guo, HZ,et al. Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,39(3-4):469-476.
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