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Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography
Liu, RX1; Wang, Q1; Guo, HZ1; Li, L1; Bi, KS1; Guo, DA1
关键词Dalbergia Odorifera Flavonoids Reversed-phase High Performance Liquid Chromatography
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2005-09-15
DOI10.1016/j.jpba.2005.04.007
39期:3-4页:469-476
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]RED-CLOVER ; BUTEIN ; CONSTITUENTS ; ISOFLAVONES ; INHIBITOR ; HEARTWOOD ; APOPTOSIS ; CELLS ; HPLC
英文摘要

A reversed-phase liquid chromatographic method was developed for the simultaneous quantification of 10 major flavonoids, namely butin, (3R)-4′-methoxy-2′,3,7-trihydroxyisoflavanone, liquiritigenin, melanettin, violanone, vistitone, formononetin, dalbergin, sativanone and medicarpin in the heartwood of Dalbergia odorifera, an important traditional Chinese medicine. Samples were extracted with 60% methanol. The optimal conditions of separation and detection were achieved on an AgilentZorbax SB-C-18 column (250mm x 4.6mm, 5 mu m) with a gradient of acetonitrile and 0.3% (v/v) aqueous acetic acid, at a flow rate of 0.8 ml/min, detected at 275 nm. The complete separation was obtained within 55 min for the 10 target compounds. All calibration curves showed good linearity (r(2) > 0.999) within test ranges. The assay was reproducible with overall intra- and inter-day variation of less than 3%. The mean recovery of the method was 100 +/- 1 0%, with R.S.D. less than 5%. The current assay method was considered to be suitable for the quality control of D. odorifera samples and could be readily utilized for the determination of the active principles present in this medicinal herb. (c) 2005 Elsevier B.V. All fights reserved.

语种英语
WOS记录号WOS:000232209700014
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50518
专题北京大学药学院
北京大学精神卫生研究所_药房
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
推荐引用方式
GB/T 7714
Liu, RX,Wang, Q,Guo, HZ,et al. Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,39(3-4):469-476.
APA Liu, RX,Wang, Q,Guo, HZ,Li, L,Bi, KS,&Guo, DA.(2005).Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,39(3-4),469-476.
MLA Liu, RX,et al."Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 39.3-4(2005):469-476.
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