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学科主题: 基础医学
题名:
Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application
作者: Yang, SM; Zhang, B; Wang, JM; Liao, SL; Han, JH; Wei, JP; Hou, L
刊名: HYBRIDOMA
发表日期: 2001-08-01
卷: 20, 期:4, 页:249-255
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: CANCER
英文摘要:

The catalytic subunit of human telomerase reverse transcriptase (hTERT), is the necessary and rate-limiting component to telomerase activation in cancer cells. To develop monoclonal antibodies (MAbs) against hTERT, a peptide-hTERT(9)-derived from specific motif T of hTERT was synthesized. Through fusion of splenocytes of BALB/c mice immunized with hTERT(9) with mouse myeloma cells, hybridomas were generated and clones secreting anti-hTERT(9) antibody were screened. After three rounds of limited dilution of candidate clones, three of which present stable and constant antibody production. The MAbs were hTERT(9)-specific and reactive with native hTERT of human cancer cells or tissues in Western blot and immunohistochemistry. The heavy chain variable regions from three hybridomas were cloned and sequenced confirming their mouse Ig derivation. The described investigation suggested that the generated MAbs to hTERT(9) could recognize native hTERT and be useful to cancer research.

语种: 英语
WOS记录号: WOS:000171628100006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50524
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: Beijing Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Yang, SM,Zhang, B,Wang, JM,et al. Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application[J]. HYBRIDOMA,2001,20(4):249-255.
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