IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application
Yang, SM; Zhang, B; Wang, JM; Liao, SL; Han, JH; Wei, JP; Hou, L
刊名HYBRIDOMA
2001-08-01
20期:4页:249-255
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]CANCER
英文摘要

The catalytic subunit of human telomerase reverse transcriptase (hTERT), is the necessary and rate-limiting component to telomerase activation in cancer cells. To develop monoclonal antibodies (MAbs) against hTERT, a peptide-hTERT(9)-derived from specific motif T of hTERT was synthesized. Through fusion of splenocytes of BALB/c mice immunized with hTERT(9) with mouse myeloma cells, hybridomas were generated and clones secreting anti-hTERT(9) antibody were screened. After three rounds of limited dilution of candidate clones, three of which present stable and constant antibody production. The MAbs were hTERT(9)-specific and reactive with native hTERT of human cancer cells or tissues in Western blot and immunohistochemistry. The heavy chain variable regions from three hybridomas were cloned and sequenced confirming their mouse Ig derivation. The described investigation suggested that the generated MAbs to hTERT(9) could recognize native hTERT and be useful to cancer research.

语种英语
WOS记录号WOS:000171628100006
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50524
专题北京大学基础医学院_病理学系
北京大学基础医学院
北京大学公共卫生学院_北京大学儿童青少年卫生研究所
北京大学第一临床医学院_儿科
作者单位Beijing Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yang, SM,Zhang, B,Wang, JM,et al. Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application[J]. HYBRIDOMA,2001,20(4):249-255.
APA Yang, SM.,Zhang, B.,Wang, JM.,Liao, SL.,Han, JH.,...&Hou, L.(2001).Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application.HYBRIDOMA,20(4),249-255.
MLA Yang, SM,et al."Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): Preparation, characterization, and application".HYBRIDOMA 20.4(2001):249-255.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Monoclonal antibodie(198KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, SM]的文章
[Zhang, B]的文章
[Wang, JM]的文章
百度学术
百度学术中相似的文章
[Yang, SM]的文章
[Zhang, B]的文章
[Wang, JM]的文章
必应学术
必应学术中相似的文章
[Yang, SM]的文章
[Zhang, B]的文章
[Wang, JM]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Monoclonal antibodies against human telomerase reverse transcriptase (hTERT) Preparation, characterization, and application.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。