学科主题基础医学
Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma
Zhang Qi1,2,3; Vuong, Helen4,5,6; Huang Xin1,2,3; Wang YanLing1,2,3; Brecha, Nicholas C.4,5,6; Pu MingLiang1,2,3; Gao Jie1,2,3
关键词pigmentary glaucoma Thy1-CFP-DBA/2J mouse retinal ganglion cells melanopsin-expressing retinal ganglion cells depression
刊名SCIENCE CHINA-LIFE SCIENCES
2013-08-01
DOI10.1007/s11427-013-4493-1
56期:8页:720-730
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]FLUORESCENT PROTEIN EXPRESSION ; SEASONAL AFFECTIVE-DISORDER ; PUPILLARY LIGHT REFLEX ; DBA/2J MICE ; PERSONALITY-TRAITS ; AMACRINE CELLS ; IRIS ATROPHY ; OLDER-ADULTS ; DEPRESSION ; ANXIETY
英文摘要

In this study, the role of melanopsin-expressing retinal ganglion cells (mRGCs) in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study. Immunohistochemical labeling, quantitative analysis of mRGC morphology, open field test (OFT), and statistical analysis were used. In comparison with C57 BL/6 mice, the age-matched CFP-D2 mice had significantly elevated intraocular pressure (IOP). We observed parallel morphological changes in the retina, including a reduction in the density of cyan fluorescent protein-(CFP) expressing cells (cells mm(-2) at 2 months of age, 1309 +/- 26; 14 months, 878 +/- 30, P < 0.001), mRGCs (2 months, 48 +/- 3; 14 months, 19 +/- 4, P < 0.001), Brn3b-expressing RGCs (2 months, 1283 +/- 80; 14 months, 950 +/- 31, P < 0.001), Brn-3b expressing mRGCs (5 months, 50.17%+/- 5.5%; 14 months, 12.61%+/- 3.8%, P < 0.001), and reduction in the dendritic field size of mRGCs (mm(2) at 2 months, 0.077 +/- 0.015; 14 months, 0.065 +/- 0.015, P < 0.05). CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled, number of entries into the center, and time spent in the center of the testing apparatus. The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs, most likely Brn-3b-positive mRGCs in CFP-D2 mice.

语种英语
WOS记录号WOS:000322886500009
项目编号2009CB320900 ; 2011CB510206 ; 30831160516 ; EY04067
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; NIH ; VA Merit Review ; PKU-UCLA Joint Research Institute
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50550
专题北京大学基础医学院_解剖学与组织胚胎学系
北京大学基础医学院
北京大学公共卫生学院_公共卫生学院
北京大学第二临床医学院_中心实验室
作者单位1.Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
2.Peking Univ, Sch Basic Med Sci, Dept Anat, Beijing 100191, Peoples R China
3.Peking Univ, Key Lab Machine Percept, Minist Educ, Beijing 100191, Peoples R China
4.Peking Univ, Key Lab Visual Impairment & Restorat, Minist Educ, Beijing 100191, Peoples R China
5.Univ Calif Los Angeles, Dept Neurobiol & Mol Cellular & Integrat Physiol, Los Angeles, CA 90095 USA
6.Univ Calif Los Angeles, Coll Life Sci, Los Angeles, CA 90095 USA
推荐引用方式
GB/T 7714
Zhang Qi,Vuong, Helen,Huang Xin,et al. Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(8):720-730.
APA Zhang Qi.,Vuong, Helen.,Huang Xin.,Wang YanLing.,Brecha, Nicholas C..,...&Gao Jie.(2013).Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma.SCIENCE CHINA-LIFE SCIENCES,56(8),720-730.
MLA Zhang Qi,et al."Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma".SCIENCE CHINA-LIFE SCIENCES 56.8(2013):720-730.
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