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学科主题: 基础医学
题名:
Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma
作者: Zhang Qi1,2,3; Vuong, Helen4,5,6; Huang Xin1,2,3; Wang YanLing1,2,3; Brecha, Nicholas C.4,5,6; Pu MingLiang1,2,3; Gao Jie1,2,3
关键词: pigmentary glaucoma ; Thy1-CFP-DBA/2J mouse ; retinal ganglion cells ; melanopsin-expressing retinal ganglion cells ; depression
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2013-08-01
DOI: 10.1007/s11427-013-4493-1
卷: 56, 期:8, 页:720-730
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: FLUORESCENT PROTEIN EXPRESSION ; SEASONAL AFFECTIVE-DISORDER ; PUPILLARY LIGHT REFLEX ; DBA/2J MICE ; PERSONALITY-TRAITS ; AMACRINE CELLS ; IRIS ATROPHY ; OLDER-ADULTS ; DEPRESSION ; ANXIETY
英文摘要:

In this study, the role of melanopsin-expressing retinal ganglion cells (mRGCs) in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study. Immunohistochemical labeling, quantitative analysis of mRGC morphology, open field test (OFT), and statistical analysis were used. In comparison with C57 BL/6 mice, the age-matched CFP-D2 mice had significantly elevated intraocular pressure (IOP). We observed parallel morphological changes in the retina, including a reduction in the density of cyan fluorescent protein-(CFP) expressing cells (cells mm(-2) at 2 months of age, 1309 +/- 26; 14 months, 878 +/- 30, P < 0.001), mRGCs (2 months, 48 +/- 3; 14 months, 19 +/- 4, P < 0.001), Brn3b-expressing RGCs (2 months, 1283 +/- 80; 14 months, 950 +/- 31, P < 0.001), Brn-3b expressing mRGCs (5 months, 50.17%+/- 5.5%; 14 months, 12.61%+/- 3.8%, P < 0.001), and reduction in the dendritic field size of mRGCs (mm(2) at 2 months, 0.077 +/- 0.015; 14 months, 0.065 +/- 0.015, P < 0.05). CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled, number of entries into the center, and time spent in the center of the testing apparatus. The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs, most likely Brn-3b-positive mRGCs in CFP-D2 mice.

语种: 英语
所属项目编号: 2009CB320900 ; 2011CB510206 ; 30831160516 ; EY04067
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; NIH ; VA Merit Review ; PKU-UCLA Joint Research Institute
WOS记录号: WOS:000322886500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50550
Appears in Collections:基础医学院_解剖学与组织胚胎学系_期刊论文

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作者单位: 1.Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
2.Peking Univ, Sch Basic Med Sci, Dept Anat, Beijing 100191, Peoples R China
3.Peking Univ, Key Lab Machine Percept, Minist Educ, Beijing 100191, Peoples R China
4.Peking Univ, Key Lab Visual Impairment & Restorat, Minist Educ, Beijing 100191, Peoples R China
5.Univ Calif Los Angeles, Dept Neurobiol & Mol Cellular & Integrat Physiol, Los Angeles, CA 90095 USA
6.Univ Calif Los Angeles, Coll Life Sci, Los Angeles, CA 90095 USA

Recommended Citation:
Zhang Qi,Vuong, Helen,Huang Xin,et al. Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(8):720-730.
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