北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学肝病研究所  > 期刊论文
学科主题: 临床医学
题名:
Transfusion of multi-factors activated immune cells as a novel treatment for patients with chronic hepatitis B
作者: Sun, J; Gao, Y; Chen, HS; Wang, SX; Li, RB; Jiang, D; Wei, L; Wang, Y
关键词: hepatitis B ; multi-factors activated immune cell ; ex vivo
刊名: JOURNAL OF CLINICAL VIROLOGY
发表日期: 2006
DOI: 10.1016/j.jcv.2005.04.006
卷: 35, 期:1, 页:26-32
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: CYTOTOXIC T-LYMPHOCYTES ; INDUCED KILLER-CELLS ; LYMPH-NODE CELLS ; ADOPTIVE IMMUNOTHERAPY ; INTERFERON-ALFA ; VIRUS-INFECTION ; INTERLEUKIN-2 ; ANTI-CD3 ; THERAPY ; CD4(+)
英文摘要:

Background and objectives: Host-virus interactions play a central role in determining the prognosis of hepatitis B virus infection. Multi-factors activated immune cells (MAICs) are autologous peripheral blood mononuclear cells activated with anti-CD3 monoclonal antibody, interleukin-2 and interferon-gamma. The present pilot clinical trial was designed to determine whether adoptively transferred MAICs inhibit the replication of hepatitis B virus and is safe for patients.

Study design: Fourteen patients with chronic hepatitis B were enrolled in the study. A total of (1.5-4.0) x 10(9) peripheral blood mononuclear cells were isolated from each patient and activated by anti-CD3 monoclonal antibody, interleukin-2 and interferon-gamma in vitro for 10 days to produce MAICs. Cell phenotypes and levels of cytokine secretion were determined during cell culture. Patients were followed up for I year after the transfusion of MAICs.

Results: After 10 days of culture, peripheral blood mononuclear cells were expanded to a mean fold of 4.68 +/- 1.78 and activated effectively, as demonstrated by CD25 expression and cytokine secretion. Cell activation peaked on day 4. All patients accepted the MAICs transfusion with some slight adverse events, and fulminant hepatitis and bilirubinemia were not observed. Significant HBV inhibition was observed in 8 out of 14 patients, in which 5 patients achieved complete response (defined as that serum HBV DNA levels below the detection limit, occurrence of HBeAg seroconversion and normalization of ALT were observed together after MAICs transfusion and kept for at least 6 months) and 3 patients achieved partial response (defined as that serum HBV DNA levels below the detection limit, occurrence of HBeAg disappearance and normalization of ALT were observed together in 6 months after MAICs transfusion, but kept for less than 6 months).

Conclusion: These findings strongly suggest that MAICs transfusion, which effectively inhibits the replication of hepatitis B virus, is safe for patients. (C) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000235182800004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50551
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hlth Sci Ctr, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Sun, J,Gao, Y,Chen, HS,et al. Transfusion of multi-factors activated immune cells as a novel treatment for patients with chronic hepatitis B[J]. JOURNAL OF CLINICAL VIROLOGY,2006,35(1):26-32.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, J]'s Articles
[Gao, Y]'s Articles
[Chen, HS]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, J]‘s Articles
[Gao, Y]‘s Articles
[Chen, HS]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace