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学科主题临床医学
Adverse factors increase preeclampsia-like changes in pregnant mice with abnormal lipid metabolism
Ding Xiaoyan; Yang Zi; Han Yiwei; Yu Huan
关键词Preeclampsia Lipid Metabolism Ng-nitroarginine Methyl Ester Apolipoprotein C-iii
刊名CHINESE MEDICAL JOURNAL
2014-08-05
DOI10.3760/cma.j.issn.0366-6999.20140450
127期:15页:2814-2818
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]EARLY-ONSET PREECLAMPSIA ; EARLY GESTATIONAL STAGE ; NITRIC-OXIDE SYNTHESIS ; MOUSE MODEL ; L-NAME ; WOMEN ; RISK ; HYPERTRIGLYCERIDEMIA ; RATS ; INHIBITION
英文摘要

Background Preeclampsia (PE) is a multifactorial pregnancy complication. Maternal underlying condition and adverse factors both influence the pathogenesis of PE. Abnormal lipid metabolism as a maternal underlying disease may participate in the occurrence and development of PE. This study aimed to observe the effects of adverse factors on PE-like symptoms of pregnant mice with genetic abnormal lipid metabolism.

Methods Apolipoprotein C-III (ApoC3) transgenic mice with abnormal lipid metabolism were subcutaneously injected with L-arginine methyl ester (L-NAME) or normal saline (NS) daily starting at Day 7 or 16 of pregnancy (ApoC3+L-NA and ApoC3+NS groups), and wild-type (WT) mice served as a control (WT+L-NA and WT+NS groups). All mice were subdivided into early and late subgroups by injection time. The mean arterial pressure (MAP) and urinary protein were measured. Pregnancy outcomes, including fetal weight, placental weight, live birth rate, and fetal absorption rate, were analyzed. Pathologic changes in the placenta were observed by hematoxylin-eosin staining. One-way analysis of variance, t-test, and chi(2) test were used for statistical analysis.

Results MAP significantly increased for ApoC3+NS groups compared with WT+NS groups (P<0.05), without significant difference in urine protein. Following L-NAME injection, MAP and urinary protein significantly increased for ApoC3+L-NA and WT+L-NA compared with the corresponding NS groups (P<0.05), and the increase for ApoC3+L-NA was more obvious. Urinary protein levels in early ApoC3+L-NA and WT+L-NA significantly increased compared with the corresponding late groups (P<0.05). Fetal absorption rate significantly increased and fetal and placental weights significantly decreased in early ApoC3+L-NA and WT+L-NA compared with the corresponding NS groups (P<0.05), without significant difference in late ApoC3+L-NA and WT+L-NA groups. Fetal weight in early ApoC3+L-NA was significantly lower than in early WT+L-NA group (P<0.05). Morphologic examination of placentas from early ApoC3+L-NA and WT+L-NA groups showed varying degrees of fibrinoid necrosis.

Conclusions ApoC3 transgenic mice with abnormal lipid metabolism showed gestational hypertension. Adverse factors and early effect time could aggravate the PE-like symptoms for ApoC3 transgenic mice.

语种英语
WOS记录号WOS:000340691000018
项目编号81370723 ; 20130001110111
资助机构National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education from the Ministry of Education of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50561
专题北京大学第三临床医学院
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ding Xiaoyan,Yang Zi,Han Yiwei,et al. Adverse factors increase preeclampsia-like changes in pregnant mice with abnormal lipid metabolism[J]. CHINESE MEDICAL JOURNAL,2014,127(15):2814-2818.
APA Ding Xiaoyan,Yang Zi,Han Yiwei,&Yu Huan.(2014).Adverse factors increase preeclampsia-like changes in pregnant mice with abnormal lipid metabolism.CHINESE MEDICAL JOURNAL,127(15),2814-2818.
MLA Ding Xiaoyan,et al."Adverse factors increase preeclampsia-like changes in pregnant mice with abnormal lipid metabolism".CHINESE MEDICAL JOURNAL 127.15(2014):2814-2818.
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