IR@PKUHSC  > 北京大学口腔医学院  > 牙周科
学科主题口腔医学
Effects of Enamel Matrix Derivative on Proliferation/Viability, Migration, and Expression of Angiogenic Factor and Adhesion Molecules in Endothelial Cells In Vitro
Bertl, Kristina1; An, Na2,4; Bruckmann, Corinna1; Dard, Michel3; Andrukhov, Oleh1; Matejka, Michael1; Rausch-Fan, Xiaohui1
关键词Angiogenesis Enamel Matrix Proteins Endothelial Cells Wound Healing
刊名JOURNAL OF PERIODONTOLOGY
2009-10-01
DOI10.1902/jop.2009.090157
80期:10页:1622-1630
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者Pre-Clinical Research ; Pre-Clinical Research
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]PERIODONTAL-LIGAMENT CELLS ; GROWTH-FACTOR-BETA ; TGF-BETA ; REGENERATION ; MODEL ; AMELOGENIN ; CULTURE ; ALPHA ; INHIBITION ; PROTEINS
英文摘要

Background: The aim of this study was to test in vitro the effect of enamel matrix derivative (EMD) on the proliferation/viability, migration, and expression of angiogenic factor and adhesion molecules in human umbilical vein endothelial cells (HUVECs). To date, discussions on angiogenic effects of EMD are rather controversial.

Methods: The effect of EMD on the proliferation/viability of HUVECs after 24 hours was measured using 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay and direct cell counting. Cell migration was observed in an especially adapted in vitro monolayer wound-healing model. The expression of angiogenic factor angiopoietin-2 (ang-2) and adhesion molecules intercellular adhesion molecule (ICAM)-1 and vascular endothelium-selectin (E-selectin) was quantified with real-time polymerase chain reaction (PCR).

Results: The proliferation/viability of HUVECs measured in MTT assay was stimulated by 0.1 mu g/ml EMD and inhibited by higher doses (50 to 100 mu g/ml), but the total number of cells was not affected. Cell migration in the wound-healing assay was promoted by EMD at doses of 0.1 to 50 mu g/ml and inhibited at 100 mu g/ml. The highest expression level of all three tested genes (ICAM-1, E-selectin, and ang-2) was observed at 50 mu g/ml EMD.

Conclusion: The results of the present in vitro study show the potential influence of EMD on the angiogenic activity of HUVECs, which may play an important role in periodontal tissue regeneration and wound healing. J Periodontol 2009;80:1622-1630.

语种英语
资助者Pre-Clinical Research ; Pre-Clinical Research
WOS记录号WOS:000270999400013
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50568
Collection北京大学口腔医学院_牙周科
作者单位1.Inst Straumann, Basel, Switzerland
2.Bernhard Gottlieb Univ, Dept Periodontol, Clin Dent, A-1090 Vienna, Austria
3.Peking Univ, Dept Periodontol, Sch Stomatol, Beijing 100871, Peoples R China
4.Hosp Stomatol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Bertl, Kristina,An, Na,Bruckmann, Corinna,et al. Effects of Enamel Matrix Derivative on Proliferation/Viability, Migration, and Expression of Angiogenic Factor and Adhesion Molecules in Endothelial Cells In Vitro[J]. JOURNAL OF PERIODONTOLOGY,2009,80(10):1622-1630.
APA Bertl, Kristina.,An, Na.,Bruckmann, Corinna.,Dard, Michel.,Andrukhov, Oleh.,...&Rausch-Fan, Xiaohui.(2009).Effects of Enamel Matrix Derivative on Proliferation/Viability, Migration, and Expression of Angiogenic Factor and Adhesion Molecules in Endothelial Cells In Vitro.JOURNAL OF PERIODONTOLOGY,80(10),1622-1630.
MLA Bertl, Kristina,et al."Effects of Enamel Matrix Derivative on Proliferation/Viability, Migration, and Expression of Angiogenic Factor and Adhesion Molecules in Endothelial Cells In Vitro".JOURNAL OF PERIODONTOLOGY 80.10(2009):1622-1630.
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