IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue
Peng, Linyi1,3; Jia, Zhuqing1; Yin, Xinhua3; Zhang, Xin2; Liu, Yinan1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
刊名STEM CELLS AND DEVELOPMENT
2008-08-01
DOI10.1089/scd.2007.0217
17期:4页:761-773
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]IN-VITRO ; STROMAL CELLS ; THERAPEUTIC ANGIOGENESIS ; DIFFERENTIATION ; VIVO ; TRANSPLANTATION ; POPULATIONS ; APOPTOSIS ; CYTOKINES ; GROWTH
英文摘要

Mesenchymal stem cells (MSCs) isolated from bone marrow (BM), cartilage, and adipose tissue (AT) possess the capacity for self-renewal and the potential for multilineage differentiation, and are therefore perceived as attractive sources of stem cells for cell therapy. However, MSCs from these different sources have different characteristics. We compared MSCs of adult Sprague Dawley rats derived from these three sources in terms of their immunophenotypic characterization, proliferation capacity, differentiation ability, expression of angiogenic cytokines, and anti-apoptotic ability. According to growth curve, cell cycle, and telomerase activity analyses, MSCs derived from adipose tissue (AT-MSCs) possess the highest proliferation potential, followed by MSCs derived from BM and cartilage (BM-MSCs and C-MSCs). In terms of multilineage differentiation, MSCs from all three sources displayed osteogenic, adipogenic, and chondrogenic differentiation potential. The result of real-time RT-PCR indicated that these cells all expressed angiogenic cytokines, with some differences in expression level. Flow cytometry and MTT analysis showed that C-MSCs possess the highest resistance toward hydrogen peroxide-induced apoptosis, while AT-MSCs exhibited high tolerance to serum deprivation-induced apoptosis. Both AT and cartilage are attractive alternatives to BM as sources for isolating MSCs, but these differences must be considered when choosing a stem cell source for clinical application.

语种英语
WOS记录号WOS:000259295600014
引用统计
被引频次:158[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50569
专题北京大学基础医学院
北京大学药学院_药学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Sport Med, Beijing 100083, Peoples R China
3.Harbin Med Univ, Hosp Affiliated 2, Dept Cardiol, Harbin, Peoples R China
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GB/T 7714
Peng, Linyi,Jia, Zhuqing,Yin, Xinhua,et al. Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue[J]. STEM CELLS AND DEVELOPMENT,2008,17(4):761-773.
APA Peng, Linyi.,Jia, Zhuqing.,Yin, Xinhua.,Zhang, Xin.,Liu, Yinan.,...&Zhou, Chunyan.(2008).Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue.STEM CELLS AND DEVELOPMENT,17(4),761-773.
MLA Peng, Linyi,et al."Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue".STEM CELLS AND DEVELOPMENT 17.4(2008):761-773.
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