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学科主题: 基础医学
题名:
Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue
作者: Peng, Linyi1,3; Jia, Zhuqing1; Yin, Xinhua3; Zhang, Xin2; Liu, Yinan1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
刊名: STEM CELLS AND DEVELOPMENT
发表日期: 2008-08-01
DOI: 10.1089/scd.2007.0217
卷: 17, 期:4, 页:761-773
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: IN-VITRO ; STROMAL CELLS ; THERAPEUTIC ANGIOGENESIS ; DIFFERENTIATION ; VIVO ; TRANSPLANTATION ; POPULATIONS ; APOPTOSIS ; CYTOKINES ; GROWTH
英文摘要:

Mesenchymal stem cells (MSCs) isolated from bone marrow (BM), cartilage, and adipose tissue (AT) possess the capacity for self-renewal and the potential for multilineage differentiation, and are therefore perceived as attractive sources of stem cells for cell therapy. However, MSCs from these different sources have different characteristics. We compared MSCs of adult Sprague Dawley rats derived from these three sources in terms of their immunophenotypic characterization, proliferation capacity, differentiation ability, expression of angiogenic cytokines, and anti-apoptotic ability. According to growth curve, cell cycle, and telomerase activity analyses, MSCs derived from adipose tissue (AT-MSCs) possess the highest proliferation potential, followed by MSCs derived from BM and cartilage (BM-MSCs and C-MSCs). In terms of multilineage differentiation, MSCs from all three sources displayed osteogenic, adipogenic, and chondrogenic differentiation potential. The result of real-time RT-PCR indicated that these cells all expressed angiogenic cytokines, with some differences in expression level. Flow cytometry and MTT analysis showed that C-MSCs possess the highest resistance toward hydrogen peroxide-induced apoptosis, while AT-MSCs exhibited high tolerance to serum deprivation-induced apoptosis. Both AT and cartilage are attractive alternatives to BM as sources for isolating MSCs, but these differences must be considered when choosing a stem cell source for clinical application.

语种: 英语
所属项目编号: 2001AA2160313 ; 104-4 ; H020220010490
项目资助者: National High Technology Research and Development Program of China ; Peking University ; Beijing Science and Technology Commission
WOS记录号: WOS:000259295600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50569
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Sport Med, Beijing 100083, Peoples R China
3.Harbin Med Univ, Hosp Affiliated 2, Dept Cardiol, Harbin, Peoples R China

Recommended Citation:
Peng, Linyi,Jia, Zhuqing,Yin, Xinhua,et al. Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue[J]. STEM CELLS AND DEVELOPMENT,2008,17(4):761-773.
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