IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Effect of ligand of peroxisome proliferator-activated receptor gamma on the biological characters of hepatic stellate cells
Guo, Yan-Tong; Leng, Xi-Sheng; Li, Tao; Peng, Ji-Run; Song, Sheng-Han; Xiong, Liang-Fa; Qin, Zhi-Zhong
关键词Peroxisome Proliferator-activated Receptor Gamma Hepatic Stellate Cell Rosiglitazone
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2005-08-14
11期:30页:4735-4739
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]TRANSCRIPTIONAL REGULATION ; MOLECULAR REGULATION ; SIGNAL-TRANSDUCTION ; PPAR-GAMMA ; RAT-LIVER ; FIBROGENESIS ; FIBROSIS ; COLLAGEN ; GROWTH ; THIAZOLIDINEDIONES
英文摘要

AIM: To study the effect of rosiglitazone, which is a ligand of peroxisome proliferator-activated receptor gamma (PPAR gamma), on the expression of PPAR gamma in hepatic stellate cells (HSCs) and on the biological characteristics of HSCs.

METHODS: The activated HSCs were divided into three groups: control group, 3 mu mol/L rosiglitazone group, and 10 mu mol/L rosiglitazone group. The expression of PPAR gamma, alpha-smooth muscle actin (alpha-SMA), and type I and III collagen was detected by RT-PCR, Western blot and immunocytochemical staining, respectively. Cell proliferation was determined with methylthiazolyltetrazolium (MTT) colorimetric assay. Cell apoptosis was demonstrated with flow cytometry.

RESULTS: The expression of PPAR gamma at mRNA and protein level markedly increased in HSCs of 10 mu mol/L rosiglitazone group (t value was 10.870 and 4.627 respectively, P<0.01 in both). The proliferation of HSCs in 10 mu mol/L rosiglitazone group decreased significantly (t = 5.542, P<0.01), alpha-SMA expression level and type I collagen synthesis ability were also reduced vs controls (t value = 10.256 and 14.627 respectively, P<0.01 in both). The apoptotic rate of HSCs significantly increased in 10 mu mol/L rosiglitazone group vs control (chi(2) = 16.682, P<0.01).

CONCLUSION: By increasing expression of PPAR gamma in activated HSCs, rosiglitazone, an agonist of PPAR gamma, decreases alpha-SMA expression and type I collagen synthesis, inhibits cell proliferation, and induces cell apoptosis. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000208099500024
项目编号30371387
资助机构National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50583
专题北京大学第二临床医学院
作者单位Peking Univ Peoples Hosp, Dept Gen Surg, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Guo, Yan-Tong,Leng, Xi-Sheng,Li, Tao,et al. Effect of ligand of peroxisome proliferator-activated receptor gamma on the biological characters of hepatic stellate cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(30):4735-4739.
APA Guo, Yan-Tong.,Leng, Xi-Sheng.,Li, Tao.,Peng, Ji-Run.,Song, Sheng-Han.,...&Qin, Zhi-Zhong.(2005).Effect of ligand of peroxisome proliferator-activated receptor gamma on the biological characters of hepatic stellate cells.WORLD JOURNAL OF GASTROENTEROLOGY,11(30),4735-4739.
MLA Guo, Yan-Tong,et al."Effect of ligand of peroxisome proliferator-activated receptor gamma on the biological characters of hepatic stellate cells".WORLD JOURNAL OF GASTROENTEROLOGY 11.30(2005):4735-4739.
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