IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
Effects of Low-Energy Shockwave Therapy on the Erectile Function and Tissue of a Diabetic Rat Model
Qiu, Xuefeng1; Lin, Guiting1; Xin, Zhongcheng2; Ferretti, Ludovic1; Zhang, Haiyang1; Lue, Tom F.1; Lin, Ching-Shwun1
关键词Low-energy Shockwave Diabetes Mellitus Erectile Dysfunction
刊名JOURNAL OF SEXUAL MEDICINE
2013-03-01
DOI10.1111/jsm.12024
10期:3页:738-746
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]NITRIC-OXIDE SYNTHASE ; ENDOTHELIAL GROWTH-FACTOR ; MESENCHYMAL STEM-CELLS ; WAVE THERAPY ; STROMAL CELLS ; DYSFUNCTION ; IDENTIFICATION ; RECRUITMENT ; ISCHEMIA
英文摘要

Introduction. Low-energy shockwave therapy (LESWT) has been shown to improve erectile function in patients suffering from diabetes mellitus (DM)-associated erectile dysfunction (ED). However, the underlying mechanism remains unknown. Aim. The aim of this study is to investigate whether LESWT can ameliorate DM-associated ED in a rat model and examine the associated changes in the erectile tissues. Methods. Newborn male rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU; 50mg/kg) for the purpose of tracking endogenous mesenchymal stem cells (MSCs). Eight weeks later, eight of these rats were randomly chosen to serve as normal control (N group). The remaining rats were injected intraperitoneally with 60mg/kg of streptozotocin (STZ) to induce DM. Eight of these rats were randomly chosen to serve as DM control (DM group), whereas another eight rats were subject to shockwave (SW) treatment (DM+SW group). Each rat in the DM+SW group received 300 shocks at energy level of 0.1mJ/mm2 and frequency of 120/minute. This procedure was repeated three times a week for 2 weeks. Another 2 weeks later, all 24 rats were evaluated for erectile function by intracavernous pressure (ICP) measurement. Afterward, their penile tissues were examined by histology. Main Outcome Measures. Erectile function was measured by ICP. Neuronal nitric oxide synthase (nNOS)-positive nerves and the endothelium were examined by immunofluorescence staining. Smooth muscle and MSCs were examined by phalloidin and EdU staining, respectively. Results. STZ treatment caused a significant decrease in erectile function and in the number of nNOS-positive nerves and in endothelial and smooth muscle contents. These DM-associated deficits were all partially but significantly reversed by LESWT. MSCs (EdU-positive cells) were significantly more numerous in DM+SW than in DM rats. Conclusion. LESWT can partially ameliorate DM-associated ED by promoting regeneration of nNOS-positive nerves, endothelium, and smooth muscle in the penis. These beneficial effects appear to be mediated by recruitment of endogenous MSCs. Qiu X, Lin G, Xin Z, Ferretti L, Zhang H, Lue TF, and Lin C-S. Effects of low-energy shockwave therapy on the erectile function and tissue of a diabetic rat model. J Sex Med 2013;10:738746.

语种英语
WOS记录号WOS:000315732900012
Citation statistics
Cited Times:60[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50586
Collection北京大学第一临床医学院_男科中心
作者单位1.Univ Calif San Francisco, Dept Urol, Knuppe Mol Urol Lab, Sch Med, San Francisco, CA 94143 USA
2.Peking Univ, Androl Ctr, Hosp 1, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Qiu, Xuefeng,Lin, Guiting,Xin, Zhongcheng,et al. Effects of Low-Energy Shockwave Therapy on the Erectile Function and Tissue of a Diabetic Rat Model[J]. JOURNAL OF SEXUAL MEDICINE,2013,10(3):738-746.
APA Qiu, Xuefeng.,Lin, Guiting.,Xin, Zhongcheng.,Ferretti, Ludovic.,Zhang, Haiyang.,...&Lin, Ching-Shwun.(2013).Effects of Low-Energy Shockwave Therapy on the Erectile Function and Tissue of a Diabetic Rat Model.JOURNAL OF SEXUAL MEDICINE,10(3),738-746.
MLA Qiu, Xuefeng,et al."Effects of Low-Energy Shockwave Therapy on the Erectile Function and Tissue of a Diabetic Rat Model".JOURNAL OF SEXUAL MEDICINE 10.3(2013):738-746.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Qiu, Xuefeng]'s Articles
[Lin, Guiting]'s Articles
[Xin, Zhongcheng]'s Articles
百度学术
百度学术Similar articles in
[Qiu, Xuefeng]'s Articles
[Lin, Guiting]'s Articles
[Xin, Zhongcheng]'s Articles
必应学术
必应学术Similar articles in
[Qiu, Xuefeng]'s Articles
[Lin, Guiting]'s Articles
[Xin, Zhongcheng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.