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学科主题: 基础医学
题名:
Spontaneous conversion between mutL and 6 bp Delta mutL in Salmonella typhimurium LT7: Association with genome diversification and possible roles in bacterial adaptation
作者: Gong, Jun1; Liu, Wei-Qiao1; Liu, Gui-Rong1; Chen, Fang1; Li, Jun-Qian1; Xu, Guo-Min1; Wang, Ling1; Johnston, Randal N.1; Eisenstark, Abraham1; Liu, Shu-Lin1
关键词: mutL ; Salmonella ; genome diversification ; bacterial adaptation ; mutator
刊名: GENOMICS
发表日期: 2007-10-01
DOI: 10.1016/j.ygeno.2007.06.009
卷: 90, 期:4, 页:542-549
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]: DNA MISMATCH REPAIR ; ESCHERICHIA-COLI MUTS ; MUTATION-RATES ; ENTERICA ; TYPHI ; SEQUENCE ; PROTEINS ; REARRANGEMENTS ; RECOMBINATION ; POPULATIONS
英文摘要:

Previously, we reported the phenomenon of genome diversification in Salmonella typhimurium LT7, i.e., individual strains derived from LT7 kept changing the genome structure by inversions, translocations, duplications, and mutations. To elucidate the genetic basis, we sequenced selected genes of the mismatch repair (MMR) system for correlations between MMR defects and genome diversification. We chose S. typhimurium LT7 mutants 8111F2 and 9052D1 for mut gene sequence analyses and found that both mutants had a deletion of one of three tandem 6-bp repeats, GCTGGC GCTGGC GCTGGC, within mutL, which was designated 6 bp Delta mutL. mutS and mutH genes were unchanged in the mutants analyzed. Some sublines of 8111F2 and 9052D1 spontaneously stopped the genome diversification process at certain stages during single-colony restreaking passages, and in these strains the 6 bp Delta mutL genotype also became wild-type mutL. We conclude that conversion between mutL and 6 bp Delta mutL occurs spontaneously and that transient defects of mutL facilitate genome diversification without leading to the accumulation of multiple detrimental genetic changes. Spontaneous conversion between mutL and 6 bp Delta mutL may be an important mechanism used by bacteria to regulate genetic stability in adaptation to changing environments. (C) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000249990500012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50596
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada
3.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada
4.Univ Missouri, Canc Res Ctr, Columbia, MO 65211 USA

Recommended Citation:
Gong, Jun,Liu, Wei-Qiao,Liu, Gui-Rong,et al. Spontaneous conversion between mutL and 6 bp Delta mutL in Salmonella typhimurium LT7: Association with genome diversification and possible roles in bacterial adaptation[J]. GENOMICS,2007,90(4):542-549.
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