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Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia
Ju RuiJun1; Huang RenJie2; Zhou Jia1; Li RuoJing1; Zhou Peng1; Zhang ZaoHua3; Xiang FeiJun4; Xu DongJin4; Liu WeiXiang4; Ma XingTian4; Zhang Qiang1; Lu WanLiang1
关键词Large-scale Separation Salidroside Marcroporous Resin Rhodiola Kirilowii Injectable Ingredient Hplc
刊名SCIENCE CHINA-CHEMISTRY
2012-07-01
DOI10.1007/s11426-011-4471-z
55期:7页:1435-1444
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PLANT RHODIOLA-SACHALINENSIS ; PURIFICATION ; ADSORPTION ; RATS ; INJURY ; ROSEA
英文摘要

The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient. Crude extract of salidroside was prepared by water-ethanol system, and purified by column chromatography of macroporous resins. Static adsorption and desorption studies were performed on six kinds of macroporous resins, and SP825 resin was chosen, followed by optimizing process parameters. The optimum sample volume, feed concentration, ratio of diameter to height, and feeding flow rate were 1.5 bed volumes (BV), 15 mg/mL, 1:10 and 1 BV/h, respectively. Dynamic desorption was performed consecutively with 8 BV of distilled water, 3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h. After three cycles in separating 3.5 tons of rhodiola kirilowii roots, salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product. This study provides a novel method to separate salidroside for injectable use.

语种英语
WOS记录号WOS:000306070500033
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50605
专题北京大学药学院
北京大学第一临床医学院_检验科
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Fujian Hlth Coll, Dept Pharm, Fuzhou 350101, Peoples R China
3.China Acad Chinese Med Sci, Beijing 100700, Peoples R China
4.Kangmei Pharmaceut Co Ltd, Puning 515300, Peoples R China
推荐引用方式
GB/T 7714
Ju RuiJun,Huang RenJie,Zhou Jia,et al. Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia[J]. SCIENCE CHINA-CHEMISTRY,2012,55(7):1435-1444.
APA Ju RuiJun.,Huang RenJie.,Zhou Jia.,Li RuoJing.,Zhou Peng.,...&Lu WanLiang.(2012).Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia.SCIENCE CHINA-CHEMISTRY,55(7),1435-1444.
MLA Ju RuiJun,et al."Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia".SCIENCE CHINA-CHEMISTRY 55.7(2012):1435-1444.
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