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学科主题: 药学
题名:
Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia
作者: Ju RuiJun1; Huang RenJie2; Zhou Jia1; Li RuoJing1; Zhou Peng1; Zhang ZaoHua3; Xiang FeiJun4; Xu DongJin4; Liu WeiXiang4; Ma XingTian4; Zhang Qiang1; Lu WanLiang1
关键词: large-scale separation ; salidroside ; marcroporous resin ; rhodiola kirilowii ; injectable ingredient ; HPLC
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2012-07-01
DOI: 10.1007/s11426-011-4471-z
卷: 55, 期:7, 页:1435-1444
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: PLANT RHODIOLA-SACHALINENSIS ; PURIFICATION ; ADSORPTION ; RATS ; INJURY ; ROSEA
英文摘要:

The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient. Crude extract of salidroside was prepared by water-ethanol system, and purified by column chromatography of macroporous resins. Static adsorption and desorption studies were performed on six kinds of macroporous resins, and SP825 resin was chosen, followed by optimizing process parameters. The optimum sample volume, feed concentration, ratio of diameter to height, and feeding flow rate were 1.5 bed volumes (BV), 15 mg/mL, 1:10 and 1 BV/h, respectively. Dynamic desorption was performed consecutively with 8 BV of distilled water, 3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h. After three cycles in separating 3.5 tons of rhodiola kirilowii roots, salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product. This study provides a novel method to separate salidroside for injectable use.

语种: 英语
项目资助者: Puning Kangmei Pharmaceutical Co., Ltd. ; Comprehensive Center for Drug Discovery and Development of Peking University
WOS记录号: WOS:000306070500033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50605
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Fujian Hlth Coll, Dept Pharm, Fuzhou 350101, Peoples R China
3.China Acad Chinese Med Sci, Beijing 100700, Peoples R China
4.Kangmei Pharmaceut Co Ltd, Puning 515300, Peoples R China

Recommended Citation:
Ju RuiJun,Huang RenJie,Zhou Jia,et al. Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia[J]. SCIENCE CHINA-CHEMISTRY,2012,55(7):1435-1444.
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