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学科主题: 临床医学
题名:
MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism
作者: Wang, Bo1; Shen, Zhan-long1; Jiang, Ke-wei1; Zhao, Gang2; Wang, Chun-you2; Yan, Yi-chao1; Yang, Yang1; Zhang, Ji-zhun1; Shen, Chao1; Gao, Zhi-dong1; Ye, Ying-jiang1; Wang, Shan1
关键词: miR-217 ; AEG-1 ; colorectal cancer ; proliferation ; invasion
刊名: BMC CANCER
发表日期: 2015-05-28
DOI: 10.1186/s12885-015-1438-z
卷: 15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: ASTROCYTE ELEVATED GENE-1 ; PANCREATIC-CANCER ; TUMOR-SUPPRESSOR ; BREAST-CANCER ; MOUSE MODEL ; EXPRESSION ; MIR-217 ; CARCINOMA ; METASTASIS ; GROWTH
英文摘要:

Background: Recent studies have indicated the possible function of miR-217 in tumorigenesis. However, the roles of miR-217 in colorectal cancer (CRC) are still largely unknown.

Methods: We examined the expression of miR-217 and AEG-1 in 50 CRC tissues and the corresponding noncancerous tissues by qRT-PCR. The clinical significance of miR-217 was analyzed. CRC cell lines with miR-217 upregulation and AEG-1 silencing were established and the effects on tumor growth in vitro and in vivo were assessed. Dual-luciferase reporter gene assays were also performed to investigate the interaction between miR-217 and AEG-1.

Results: Our data demonstrated that miR-217 was significantly downregulated in 50 pairs of colorectal cancer tissues. MiR-217 expression levels were closely correlated with tumor differentiation. Moreover, decreased miR-217 expression was also associated with shorter overall survival of CRC patients. MiR-217 overexpression significantly inhibited proliferation, colony formation and invasiveness of CRC cells by promoting apoptosis and G0/G1 phase arrest. Interestingly, ectopic miR-217 expression decreased AEG-1 expression and repressed luciferase reporter activity associated with the AEG-1 3′-untranslated region (UTR). AEG-1 silencing resulted in similar biological behavior changes to those associated with miR-217 overexpression. Finally, in a nude mouse xenografted tumor model, miR-217 overexpression significantly suppressed CRC cell growth.

Conclusions: Our findings suggest that miR-217 has considerable value as a prognostic marker and potential therapeutic target in CRC.

语种: 英语
所属项目编号: 81372290 ; 81372291 ; RDB 2013-15
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Fund
WOS记录号: WOS:000355169600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50613
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Pancreat Dis Inst, Wuhan 430074, Peoples R China

Recommended Citation:
Wang, Bo,Shen, Zhan-long,Jiang, Ke-wei,et al. MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism[J]. BMC CANCER,2015,15.
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