|MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism|
|Wang, Bo1; Shen, Zhan-long1; Jiang, Ke-wei1; Zhao, Gang2; Wang, Chun-you2; Yan, Yi-chao1; Yang, Yang1; Zhang, Ji-zhun1; Shen, Chao1; Gao, Zhi-dong1; Ye, Ying-jiang1; Wang, Shan1|
|关键词||Mir-217 Aeg-1 Colorectal Cancer Proliferation Invasion|
|WOS标题词||Science & Technology|
|关键词[WOS]||ASTROCYTE ELEVATED GENE-1 ; PANCREATIC-CANCER ; TUMOR-SUPPRESSOR ; BREAST-CANCER ; MOUSE MODEL ; EXPRESSION ; MIR-217 ; CARCINOMA ; METASTASIS ; GROWTH|
Background: Recent studies have indicated the possible function of miR-217 in tumorigenesis. However, the roles of miR-217 in colorectal cancer (CRC) are still largely unknown.
Methods: We examined the expression of miR-217 and AEG-1 in 50 CRC tissues and the corresponding noncancerous tissues by qRT-PCR. The clinical significance of miR-217 was analyzed. CRC cell lines with miR-217 upregulation and AEG-1 silencing were established and the effects on tumor growth in vitro and in vivo were assessed. Dual-luciferase reporter gene assays were also performed to investigate the interaction between miR-217 and AEG-1.
Results: Our data demonstrated that miR-217 was significantly downregulated in 50 pairs of colorectal cancer tissues. MiR-217 expression levels were closely correlated with tumor differentiation. Moreover, decreased miR-217 expression was also associated with shorter overall survival of CRC patients. MiR-217 overexpression significantly inhibited proliferation, colony formation and invasiveness of CRC cells by promoting apoptosis and G0/G1 phase arrest. Interestingly, ectopic miR-217 expression decreased AEG-1 expression and repressed luciferase reporter activity associated with the AEG-1 3′-untranslated region (UTR). AEG-1 silencing resulted in similar biological behavior changes to those associated with miR-217 overexpression. Finally, in a nude mouse xenografted tumor model, miR-217 overexpression significantly suppressed CRC cell growth.
Conclusions: Our findings suggest that miR-217 has considerable value as a prognostic marker and potential therapeutic target in CRC.
|项目编号||81372290 ; 81372291 ; RDB 2013-15|
|资助机构||National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Fund|
|作者单位||1.Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China|
2.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Pancreat Dis Inst, Wuhan 430074, Peoples R China
|Wang, Bo,Shen, Zhan-long,Jiang, Ke-wei,et al. MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism[J]. BMC CANCER,2015,15.|
|APA||Wang, Bo.,Shen, Zhan-long.,Jiang, Ke-wei.,Zhao, Gang.,Wang, Chun-you.,...&Wang, Shan.(2015).MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism.BMC CANCER,15.|
|MLA||Wang, Bo,et al."MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism".BMC CANCER 15(2015).|