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IR@PKUHSC  > 北京大学第三临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Comparative study on circulating endothelial progenitor cells in systemic lupus erythematosus patients at active stage
作者: Deng, Xiao Li1; Li, Xiao Xia2; Liu, Xiang Yuan1; Sun, Lin1; Liu, Rui1
关键词: Endothelial progenitor cell ; Lupus ; Function
刊名: RHEUMATOLOGY INTERNATIONAL
发表日期: 2010-09-01
DOI: 10.1007/s00296-009-1156-4
卷: 30, 期:11, 页:1429-1436
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: RISK-FACTORS ; DISEASE ; MECHANISMS ; ATHEROSCLEROSIS ; ANGIOGENESIS ; INFLAMMATION ; INDUCTION ; ADHESION
英文摘要:

Circulating endothelial progenitor cells (CEPCs) play an important role in the process of atherosclerosis. Most previous studies on CEPC in systemic lupus erythematosus (SLE) patients were on their number and some functions and the results were not consistent. No studies on their anti-inflammatory function and integrated status were reported. The purpose of this study was to determine the number, function (including anti-inflammatory function), and the integrated status of CEPCs in active SLE patients. The study was performed in 35 active SLE patients (28 females, 7 males) and 35 age-and gender-matched healthy controls. CEPC number was determined by Fluorescence-Activated Cell Sorting. Proliferation capacity of CEPC was assessed by PCNA staining. Adhesion capacity of CEPC to fibronectin and adhesion capacity of THP1 cell to CEPC were determined by cell adhesion assay. Migratory capacity of CEPC was measured by transwell chamber assay and the potential to form tubes on Matrigel of CEPC was determined by in vivo tube formation on Matrigel test. The expression of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) assessed by quantitative PCR as well as the expression of intercellular adhesion molecule-1 (ICAM-1) and phosphorylated-Akt (p-Akt) assessed by western-blotting were used to evaluate the anti-inflammatory function and cell status of CEPCs. The number of CEPC in SLE patients was not different from that in control (p > 0.05). Proliferation capacity of CEPC was decreased in active SLE patients (p = 0.027). Adhesion capacity of CEPC to fibronectin was decreased (p = 0.04) in SLE patients and adhesion capacity of THP1 cell to CEPC was increased in SLE patients (p < 0.001). Migratory activity was reduced in patient CEPCs (p < 0.001). Capacity of CEPCs to form tube on Matrigel was decreased in SLE patients (p < 0.001). Expression of iNOS and IL-6 (p < 0.001, p = 0.006, respectively) and ICAM-1 were increased in CEPC of SLE patients and expression of p-Akt was decreased in CEPC of SLE patients. Our data show that CEPC number in active SLE patients was not significantly different from healthy controls, but their functions were partly impaired, including proliferation, adhesion, migration, and tube formation. Bad cell status and increased susceptibility to inflammatory process of CEPCs in active SLE were also observed in our study.

语种: 英语
所属项目编号: 30600209
项目资助者: Seed Foundation of Peking University Third Hospital ; Key Program Foundation of Peking University Third Hospital ; National Natural Science Foundation of China
WOS记录号: WOS:000281254400003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50628
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China

Recommended Citation:
Deng, Xiao Li,Li, Xiao Xia,Liu, Xiang Yuan,et al. Comparative study on circulating endothelial progenitor cells in systemic lupus erythematosus patients at active stage[J]. RHEUMATOLOGY INTERNATIONAL,2010,30(11):1429-1436.
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