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IR@PKUHSC  > 北京大学口腔医学院  > 口腔预防保健科  > 期刊论文
学科主题: 口腔医学
题名:
Kinetic and site-directed mutagenesis studies of Prevotella intermedia acid phosphatase
作者: Chen, XC1; Ansai, T1; Barik, S1; Takehara, T1
刊名: PROTEIN AND PEPTIDE LETTERS
发表日期: 2003-02-01
卷: 10, 期:1, 页:53-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PHOSPHOTYROSYL PHOSPHATASE ; CURVULARIA-INAEQUALIS ; ACTIVE-SITE ; GENE ; IDENTIFICATION ; SEQUENCE ; CLONING ; GLUCOSE-6-PHOSPHATASE ; CHLOROPEROXIDASE ; EXPRESSION
英文摘要:

Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis.

语种: 英语
WOS记录号: WOS:000181186600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50629
Appears in Collections:北京大学口腔医学院_口腔预防保健科_期刊论文

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作者单位: 1.Kyushu Dent Coll, Dept Prevent Dent, Kitakyushu, Fukuoka 8038580, Japan
2.Peking Univ, Sch Stomatol, Dept Prevent Dent, Beijing 100081, Peoples R China
3.Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, Mobile, AL 36688 USA

Recommended Citation:
Chen, XC,Ansai, T,Barik, S,et al. Kinetic and site-directed mutagenesis studies of Prevotella intermedia acid phosphatase[J]. PROTEIN AND PEPTIDE LETTERS,2003,10(1):53-59.
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