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学科主题: 基础医学
题名:
Expression and distribution of immunoglobulin G in the normal liver, hepatocarcinoma and postpartial hepatectomy liver
作者: Lei, Yu1; Huang, Tao1,2; Su, Meng1; Luo, Jin1; Korteweg, Christine1; Li, Jing1; Chen, Zhengshan1; Qiu, Yamei1; Liu, Xingmu1; Yan, Meiling1; Wang, Yun1; Gu, Jiang1,2
刊名: LABORATORY INVESTIGATION
发表日期: 2014-11-01
DOI: 10.1038/labinvest.2014.114
卷: 94, 期:11, 页:1283-1295
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pathology
研究领域[WOS]: Research & Experimental Medicine ; Pathology
关键词[WOS]: CANCER-CELL LINES ; ESCHERICHIA-COLI ; HUMAN BILE ; IGA ; TRANSPLANTATION ; SERUM ; PROLIFERATION ; REGENERATION ; HEPATOCYTES ; SPECIFICITY
英文摘要:

The liver has the extraordinary properties of regeneration and immune tolerance; however, the mechanisms governing these abilities are poorly understood. To address these questions, we examined the possible expression of immunoglobulins in the human and rat liver and the relationship of IgG expression to hepatocyte proliferation, metastasis, apoptosis and immune tolerance. Immunohistochemistry, in situ hybridization, laser-guided microdissection and reverse transcription-PCR were performed to examine the expression of IgG in normal human and rat liver, severe combined immunodeficient mouse (SCID) liver and human liver cancers and corresponding cell lines. Small interfering RNA (siRNA) was transfected into cultured hepatocarcinoma cells to downregulate the expression of IgG heavy chain genes. Cell proliferation and apoptosis were assayed with flow cytometry. Cell metastasis was assayed with a Transwell cell assay. Partial hepatectomy (70%) was performed in rats to examine the relationship between hepatocyte IgG and hepatocyte proliferation. IgG, together with essential enzymes for its synthesis, were expressed in the cytoplasm of hepatocytes of normal adult human and hepatoma patients and rat livers, SCID mouse liver and BRL-3A, L-02 and HepG-2 cell lines. Downregulation of IgG inhibited cell proliferation and metastasis and promoted apoptosis. Postsurgery livers expressed significantly more IgG than the livers before surgery and decreased to the original levels when hepatocytes stopped regeneration. IgA and IgM but not IgD and IgE were also positive in hepatocytes. Our findings demonstrate that normal and malignant hepatocytes are capable of synthesizing immunoglobulin, which has important roles in hepatocyte proliferation, apoptosis and cancer growth with profound clinical implications.

语种: 英语
所属项目编号: 81030033 ; 30971150 ; 81001199 ; 30950110335
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000344134500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50655
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Shantou Univ, Coll Med, Dept Pathol, Prov Key Lab Infect Dis & Immunopathol, Shantou 515041, Guangdong, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Lei, Yu,Huang, Tao,Su, Meng,et al. Expression and distribution of immunoglobulin G in the normal liver, hepatocarcinoma and postpartial hepatectomy liver[J]. LABORATORY INVESTIGATION,2014,94(11):1283-1295.
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