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学科主题: 基础医学
题名:
Upregulated expression of intermedin and its receptor in the myocardium and aorta in spontaneously hypertensive rats
作者: Zeng, Qiang1; Yuan, Ying1; Wang, Xi1; Wu, Hong Mei1; Fan, Li1; Qi, Yong Fen2; Tang, Chao Shu2; Cai, Yan2; Pan, Chun Shui2
关键词: Spontaneously hypertensive rats ; Intermedin/adrenomedullin 2 ; Calcitonin receptor-like receptor ; Receptor activity-modifying proteins ; cAMP
刊名: PEPTIDES
发表日期: 2009-02-01
DOI: 10.1016/j.peptides.2008.10.021
卷: 30, 期:2, 页:391-399
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: ACTIVITY-MODIFYING PROTEINS ; GENE-RELATED PEPTIDE ; ADRENOMEDULLIN RECEPTORS ; ISCHEMIA/REPERFUSION INJURY ; HEART-FAILURE ; INTERMEDIN/ADRENOMEDULLIN-2 ; CALCITONIN ; CELLS ; PROADRENOMEDULLIN ; SECRETION
英文摘要:

Intermedin (IMD), also called adrenomedullin 2 (ADM2), is a 47-amino acid peptide belonging to the calcitonin/calcitonin gene-related peptide (CGRP) family. IMD has similar or more potent vasodilatory and hypotensive actions compared with adrenomedullin (ADM) and CGRP. This study was designed to explore the role of IMD and its receptor in the pathogenesis of spontaneous hypertension and cardiac hypertrophy. Radioimmunoassay was employed to determine plasma immunoreactive IMD concentration and tissue immunoreactive IMD levels in the myocardium and aorta as well as cAMP concentration in the cardiovascular tissues in 13-week-old Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). The mRNA expression of IMD, its receptor, calcitonin receptor-like receptor (CRLR) and receptor activity-modifying proteins (RAMP)) were determined by semi-quantitative RT-PCR. Protein levels of CRLR and RAMPs were assayed by Western blotting. Our results showed that immunoreactive IMID concentration was enhanced in the SHR myocardium, aortas and plasma. Both the mRNA and protein levels of IMD, as well as those of CRLR and RAMP 1-3 were upregulated in SHRs. IMD affected cAMP generation in the myocardium and aorta, which were not attenuated by prior addition of either CGRP8-37 or ADM22-52 alone. These results indicate that the elevation of IMD and its receptor in the cardiovascular tissue may play an important role in the pathogenesis of spontaneous hypertension. (C) 2008 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30570738 ; 7052041
项目资助者: National Natural Sciences Foundation of China ; Beijing Natural Science Foundation of the People&prime ; s Republic of China
WOS记录号: WOS:000263447700025
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50666
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Chinese Peoples Liberat Army Gen Hosp, Dept Geriatr Cardiol 2, Beijing 100853, Peoples R China
2.Peking Univ, Dept Physiol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Zeng, Qiang,Yuan, Ying,Wang, Xi,et al. Upregulated expression of intermedin and its receptor in the myocardium and aorta in spontaneously hypertensive rats[J]. PEPTIDES,2009,30(2):391-399.
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