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学科主题: 临床医学
题名:
Use of a calcium tracer to detect stone increments in a rat calcium oxalate xenoplantation model
作者: Wang, Shuo1; Xu, Qingquan1; Huang, Xiaobo1; Lin, Jingxing2; Wang, Jinxing2; Wang, Xiaofeng1
关键词: fluorochromes ; calcium oxalation ; rat ; xenoplantation
刊名: EXPERIMENTAL AND THERAPEUTIC MEDICINE
发表日期: 2013-10-01
DOI: 10.3892/etm.2013.1233
卷: 6, 期:4, 页:957-960
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: KIDNEY-STONES ; NEPHROLITHIASIS ; BONE ; FLUORESCENCE ; PATHOGENESIS ; DISEASE
英文摘要:

The majority of urinary stones have been observed to grow by circular increments in the clinic and in animal studies. However, the mechanism of stone formation has not yet been elucidated. Marking the stone at specific time-points during the growth of the stone is likely to enable the clarification of the mechanisms behind lithogenesis. The objective of this study was to evaluate the role and efficacy of calcium-tracing fluorescence in the labeling of stone lamination in a rat calcium oxalate xenoplantation model. In the rat calcium oxalate xenoplantation model, human renal stone particles, extracted by percutaneous nephrolithotomy, were xenoplanted into the bladders of Wistar rats in a sterile manner. The rats received 1% ethylene glycol in their drinking water, starting from the day following the stone xenoplantation. Two weeks subsequent to this, three calcium-tracing fluorochromes, alizarin complexone, calcein and xylenol orange were administered by intraperitoneal injection. The newly-formed bladder stones were cut into slices and examined using light and fluorescence microscopy. The newly-formed bladder stones had a large variance in size, and circular increments were observed in the sections of the stones. The stones were successfully labeled with calcein and alizarin complexone, although calcein labeling provided superior results. However, the use of xylenol orange did not result in clear labeling. The calcium-tracing fluorochromes, calcein and alizarin complexone may be effectively used to label stone lamination in rat models.

语种: 英语
WOS记录号: WOS:000323724100019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50670
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Chinese Acad Geol Sci, Inst Geol, Beijing 100037, Peoples R China

Recommended Citation:
Wang, Shuo,Xu, Qingquan,Huang, Xiaobo,et al. Use of a calcium tracer to detect stone increments in a rat calcium oxalate xenoplantation model[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2013,6(4):957-960.
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