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学科主题: 临床医学
题名:
Identification of proteins responding to adrenergic receptor subtype-specific hypertrophy in cardiomyocytes by proteomic approaches
作者: Liu, Ning1; Gong, Kai-zheng2,3,4,5; Cai, Yuan-bin2,3,4; Li, Zijian2,3,4
关键词: adrenergic receptors ; cardiac hypertrophy ; proteomics ; reactive oxygen species
刊名: BIOCHEMISTRY-MOSCOW
发表日期: 2011-10-01
DOI: 10.1134/S0006297911100075
卷: 76, 期:10, 页:1140-1146
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NEONATAL-RAT CARDIOMYOCYTES ; REVEALS ; PATHWAY ; HEART
英文摘要:

The individual signaling pathways underlying cardiac hypertrophy, which is induced by either alpha or beta adrenergic receptor (AR), are different. Activation of different AR subtypes couples to different G proteins and induction of specific signaling pathways, which ultimately results in subtype-specific regulation of cardiac function. We present the first proteomics study identifying proteins that are related to AR subtype-specific hypertrophy in cardiomyocytes by comparing the two-dimensional electrophoresis patterns between neonatal rat cardiomyocytes treated by phenylepinephrin (PE) and by isoproterenol (ISO). An improved 2-DE strategy was used in these comparative experiments. Twenty-five differentially expressed proteins in cardiomyocytes treated by PE or treated by ISO were successfully analyzed and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry, especially those that might be responsible to intracellular oxidative stress such as dismutase, peroxiredoxin, and thioredoxin-like protein p46. In addition, induced reactive oxygen species were also found to be AR subtype-specifically relevant to endoplasmic reticulum proteinase ERK1/2 phosphorylation during the development of hypertrophy induced by different AR subtypes. The results will help to better understand the underlying mechanisms of different adrenergic receptor subtype-induced hypertrophy.

语种: 英语
所属项目编号: 2011CB503903 ; 81070078 ; 21175055 ; 20090461 ; 20110739 ; 2011713 ; 450060445316 ; 7102158
项目资助者: Major State Basic Research Development Program of China (973 Program) ; National Science Foundation of China ; Jilin Province Science and Technology Department ; Basic Research Foundation of Jilin University ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000295845100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50672
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Hlth, Key Lab Mol Cardiol, Beijing 100091, Peoples R China
2.Jilin Univ, Cent Lab, Hosp 2, Changchun 130041, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100091, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100091, Peoples R China
5.First Peoples Hosp Yangzhou, Dept Cardiol, Yangzhou 225001, Peoples R China

Recommended Citation:
Liu, Ning,Gong, Kai-zheng,Cai, Yuan-bin,et al. Identification of proteins responding to adrenergic receptor subtype-specific hypertrophy in cardiomyocytes by proteomic approaches[J]. BIOCHEMISTRY-MOSCOW,2011,76(10):1140-1146.
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